The effect of dose on the intensity of dependence in morphine-dependent mice

Introduction: Animal behaviour research is an important tool to study the addiction properties of addictive substances. The Conditioned Place Preference (CPP)paradigm is among the model used to achieve this purpose. It can be used to investigate a wide range of addiction related behaviour including...

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Main Authors: Jusoh, Ahmad Jamaluddin Al-Qasami, Syd Mohmad Faudzi, Syed Mohd Syahmi, Suhaimi, Maryam Saadah, Hashim, Ridzwan, Ridzwan, Irna Elina
Format: Conference or Workshop Item
Language:English
English
Published: 2015
Subjects:
Online Access:http://irep.iium.edu.my/45949/
http://irep.iium.edu.my/45949/7/PPoint_UCSI_%28S%29.pdf
http://irep.iium.edu.my/45949/10/45949.pdf
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spelling iium-459492015-12-18T00:37:57Z http://irep.iium.edu.my/45949/ The effect of dose on the intensity of dependence in morphine-dependent mice Jusoh, Ahmad Jamaluddin Al-Qasami Syd Mohmad Faudzi, Syed Mohd Syahmi Suhaimi, Maryam Saadah Hashim, Ridzwan Ridzwan, Irna Elina RS Pharmacy and materia medica Introduction: Animal behaviour research is an important tool to study the addiction properties of addictive substances. The Conditioned Place Preference (CPP)paradigm is among the model used to achieve this purpose. It can be used to investigate a wide range of addiction related behaviour including intoxication and relapse potential. Since it involves a long cycle experiment, it is important to optimise the experimental conditions, but minimising any unnecessity. Therefore, in this study we aim to investigate the effects of different morphine dose (3.75 - 10 mg/kg) towards intensity of morphine dependence in adult Swiss albino mice. Methods: The mice were divided into four group (n=10-12 for each group), where each group received different doses of morphine and saline on alternate day for 6 days during conditioning phase. The intensity of dependence was measured during post-conditioning phase where the preference at the drug-paired compartment was calculated during a 15 minutes observation. Results: The result has shown that three out of four groups significantly developed dependence (P < 0.05). However, the group of mice that received highest morphine dose (10 mg/kg) did not develop dependence. When baseline was compared, it was found that the mice that received the lowest morphine treatment (3.75 mg/kg) shows the highest increment from their baseline (164.7±33.3 seconds), while the group that received 10 mg/kg morphine shows the lowest increment (119.0±31.6 seconds). The trend of increment at the drug-paired compartment shows a declining trend when the dose of morphine is increased. Conclusion: Therefore, it is concluded that the optimum dose of morphine that should be administered to the adult Swiss albino mice is around 3.75 mg/kg, provided that all other experimental conditions and protocols are maintained. 2015-10-10 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/45949/7/PPoint_UCSI_%28S%29.pdf application/pdf en http://irep.iium.edu.my/45949/10/45949.pdf Jusoh, Ahmad Jamaluddin Al-Qasami and Syd Mohmad Faudzi, Syed Mohd Syahmi and Suhaimi, Maryam Saadah and Hashim, Ridzwan and Ridzwan, Irna Elina (2015) The effect of dose on the intensity of dependence in morphine-dependent mice. In: Health and Pharmaceutical Sciences Undergraduate Research Symposium, 10th October, 2015, Le Quadri Ball Room, KL campus, North Wing, UCSI University, KL, Malaysia. (Unpublished)
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Jusoh, Ahmad Jamaluddin Al-Qasami
Syd Mohmad Faudzi, Syed Mohd Syahmi
Suhaimi, Maryam Saadah
Hashim, Ridzwan
Ridzwan, Irna Elina
The effect of dose on the intensity of dependence in morphine-dependent mice
description Introduction: Animal behaviour research is an important tool to study the addiction properties of addictive substances. The Conditioned Place Preference (CPP)paradigm is among the model used to achieve this purpose. It can be used to investigate a wide range of addiction related behaviour including intoxication and relapse potential. Since it involves a long cycle experiment, it is important to optimise the experimental conditions, but minimising any unnecessity. Therefore, in this study we aim to investigate the effects of different morphine dose (3.75 - 10 mg/kg) towards intensity of morphine dependence in adult Swiss albino mice. Methods: The mice were divided into four group (n=10-12 for each group), where each group received different doses of morphine and saline on alternate day for 6 days during conditioning phase. The intensity of dependence was measured during post-conditioning phase where the preference at the drug-paired compartment was calculated during a 15 minutes observation. Results: The result has shown that three out of four groups significantly developed dependence (P < 0.05). However, the group of mice that received highest morphine dose (10 mg/kg) did not develop dependence. When baseline was compared, it was found that the mice that received the lowest morphine treatment (3.75 mg/kg) shows the highest increment from their baseline (164.7±33.3 seconds), while the group that received 10 mg/kg morphine shows the lowest increment (119.0±31.6 seconds). The trend of increment at the drug-paired compartment shows a declining trend when the dose of morphine is increased. Conclusion: Therefore, it is concluded that the optimum dose of morphine that should be administered to the adult Swiss albino mice is around 3.75 mg/kg, provided that all other experimental conditions and protocols are maintained.
format Conference or Workshop Item
author Jusoh, Ahmad Jamaluddin Al-Qasami
Syd Mohmad Faudzi, Syed Mohd Syahmi
Suhaimi, Maryam Saadah
Hashim, Ridzwan
Ridzwan, Irna Elina
author_facet Jusoh, Ahmad Jamaluddin Al-Qasami
Syd Mohmad Faudzi, Syed Mohd Syahmi
Suhaimi, Maryam Saadah
Hashim, Ridzwan
Ridzwan, Irna Elina
author_sort Jusoh, Ahmad Jamaluddin Al-Qasami
title The effect of dose on the intensity of dependence in morphine-dependent mice
title_short The effect of dose on the intensity of dependence in morphine-dependent mice
title_full The effect of dose on the intensity of dependence in morphine-dependent mice
title_fullStr The effect of dose on the intensity of dependence in morphine-dependent mice
title_full_unstemmed The effect of dose on the intensity of dependence in morphine-dependent mice
title_sort effect of dose on the intensity of dependence in morphine-dependent mice
publishDate 2015
url http://irep.iium.edu.my/45949/
http://irep.iium.edu.my/45949/7/PPoint_UCSI_%28S%29.pdf
http://irep.iium.edu.my/45949/10/45949.pdf
first_indexed 2023-09-18T21:05:23Z
last_indexed 2023-09-18T21:05:23Z
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