An investigation into the antiobesity effects of morinda citrifolia l. leaf extract in high fat diet induced obese rats using a 1H NMR metabolomics approach
Theprevalence of obesity is increasingworldwide,with high fat diet (HFD) as one of themain contributing factors.Obesity increases the predisposition to other diseases such as diabetes through various metabolic pathways. Limited availability of antiobesity drugs and the popularity of complementary...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Hindawi Publishing Corporation
2016
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/45851/ http://irep.iium.edu.my/45851/ http://irep.iium.edu.my/45851/ http://irep.iium.edu.my/45851/1/45851.pdf http://irep.iium.edu.my/45851/4/45851_An%20investigation%20into%20the%20antiobesity_Scopus.pdf |
| Summary: | Theprevalence of obesity is increasingworldwide,with high fat diet (HFD) as one of themain contributing factors.Obesity increases
the predisposition to other diseases such as diabetes through various metabolic pathways. Limited availability of antiobesity drugs
and the popularity of complementary medicine have encouraged research in finding phytochemical strategies to this multifaceted
disease. HFD induced obese Sprague-Dawley rats were treated with an extract of Morinda citrifolia L. leaves (MLE 60). After
9 weeks of treatment, positive effects were observed on adiposity, fecal fat content, plasma lipids, and insulin and leptin levels.
The inducement of obesity and treatment with MLE 60 on metabolic alterations were then further elucidated using a 1H NMR
based metabolomics approach. Discriminating metabolites involved were products of various metabolic pathways, including
glucose metabolism and TCA cycle (lactate, 2-oxoglutarate, citrate, succinate, pyruvate, and acetate), amino acid metabolism
(alanine, 2-hydroxybutyrate), choline metabolism (betaine), creatinine metabolism (creatinine), and gut microbiome metabolism
(hippurate, phenylacetylglycine, dimethylamine, and trigonelline). Treatment with MLE 60 resulted in significant improvement
in the metabolic perturbations caused obesity as demonstrated by the proximity of the treated group to the normal group in the
OPLS-DA score plot and the change in trajectory movement of the diseased group towards the healthy group upon treatment. |
|---|