Phenolic content screening and in vitro antioxidant evaluation for radical scavenging activity of methanolic cladodes extract of Opuntia cochenillifera(L.) Mill

Phenolic content screening and in vitro antioxidant evaluation for radical scavenging activity of methanolic cladodes extract of Opuntia cochenillifera(L.) Mill

Opuntia cochenilifera is a tropical cactus from the family Cactaceae. It is commonly planted as an ornamental plant in Malaysia.The objectives of this study were to screen for phenolic content, optimize chromatographic system for thin layer chromatography (TLC) analysis and evaluate the antioxida...

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Main Authors: Osman, Muhammad Faris, Mat So'ad, Siti Zaiton
Format: Article
Language:English
Published: Malaysian Pharmaceutical Society 2015
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/45838/
http://irep.iium.edu.my/45838/
http://irep.iium.edu.my/45838/9/45838.pdf
id iium-45838
recordtype eprints
spelling iium-458382016-10-21T01:56:40Z http://irep.iium.edu.my/45838/ Phenolic content screening and in vitro antioxidant evaluation for radical scavenging activity of methanolic cladodes extract of Opuntia cochenillifera(L.) Mill Osman, Muhammad Faris Mat So'ad, Siti Zaiton QD Chemistry Opuntia cochenilifera is a tropical cactus from the family Cactaceae. It is commonly planted as an ornamental plant in Malaysia.The objectives of this study were to screen for phenolic content, optimize chromatographic system for thin layer chromatography (TLC) analysis and evaluate the antioxidant activity of methanolic cladodes extract of Opuntia cochenillifera (L.) Mill. by using DPPH radical scavenging method. Observation of the chromatograms under visible light showed that the best compounds separation was achieved with the chromatographic system of chloroform-methanol with ratio 90:10. The presence of phenolic compounds were detected by staining the chromatogram with 10% methanolic ferric chloride reagent. At the highest concentration tested (1 mg/mL), methanolic cladodes extract of Opuntia cochenillifera showed 41.5% DPPH radical inhibition in comparison with gallic acid, which showed 95.2% DPPH radical inhibition. The results clearly indicated that the free radical scavenging activity of methanolic cladodes extract of Opuntia cochenillifera was lower than the standard gallic acid. The current study provides important baseline information to explore the potential use of Opuntia cochenillifera as an ingredient in local nutraceutical and cosmeceutical products. Malaysian Pharmaceutical Society 2015-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/45838/9/45838.pdf Osman, Muhammad Faris and Mat So'ad, Siti Zaiton (2015) Phenolic content screening and in vitro antioxidant evaluation for radical scavenging activity of methanolic cladodes extract of Opuntia cochenillifera(L.) Mill. Malaysian Journal of Pharmacy, 2 (1 (Supplement)). p. 145. ISSN 1675-3666 http://www.mps.org.my/index.cfm?&menuid=146
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
Osman, Muhammad Faris
Mat So'ad, Siti Zaiton
Phenolic content screening and in vitro antioxidant evaluation for radical scavenging activity of methanolic cladodes extract of Opuntia cochenillifera(L.) Mill
description Opuntia cochenilifera is a tropical cactus from the family Cactaceae. It is commonly planted as an ornamental plant in Malaysia.The objectives of this study were to screen for phenolic content, optimize chromatographic system for thin layer chromatography (TLC) analysis and evaluate the antioxidant activity of methanolic cladodes extract of Opuntia cochenillifera (L.) Mill. by using DPPH radical scavenging method. Observation of the chromatograms under visible light showed that the best compounds separation was achieved with the chromatographic system of chloroform-methanol with ratio 90:10. The presence of phenolic compounds were detected by staining the chromatogram with 10% methanolic ferric chloride reagent. At the highest concentration tested (1 mg/mL), methanolic cladodes extract of Opuntia cochenillifera showed 41.5% DPPH radical inhibition in comparison with gallic acid, which showed 95.2% DPPH radical inhibition. The results clearly indicated that the free radical scavenging activity of methanolic cladodes extract of Opuntia cochenillifera was lower than the standard gallic acid. The current study provides important baseline information to explore the potential use of Opuntia cochenillifera as an ingredient in local nutraceutical and cosmeceutical products.
format Article
author Osman, Muhammad Faris
Mat So'ad, Siti Zaiton
author_facet Osman, Muhammad Faris
Mat So'ad, Siti Zaiton
author_sort Osman, Muhammad Faris
title Phenolic content screening and in vitro antioxidant evaluation for radical scavenging activity of methanolic cladodes extract of Opuntia cochenillifera(L.) Mill
title_short Phenolic content screening and in vitro antioxidant evaluation for radical scavenging activity of methanolic cladodes extract of Opuntia cochenillifera(L.) Mill
title_full Phenolic content screening and in vitro antioxidant evaluation for radical scavenging activity of methanolic cladodes extract of Opuntia cochenillifera(L.) Mill
title_fullStr Phenolic content screening and in vitro antioxidant evaluation for radical scavenging activity of methanolic cladodes extract of Opuntia cochenillifera(L.) Mill
title_full_unstemmed Phenolic content screening and in vitro antioxidant evaluation for radical scavenging activity of methanolic cladodes extract of Opuntia cochenillifera(L.) Mill
title_sort phenolic content screening and in vitro antioxidant evaluation for radical scavenging activity of methanolic cladodes extract of opuntia cochenillifera(l.) mill
publisher Malaysian Pharmaceutical Society
publishDate 2015
url http://irep.iium.edu.my/45838/
http://irep.iium.edu.my/45838/
http://irep.iium.edu.my/45838/9/45838.pdf
first_indexed 2023-09-18T21:05:14Z
last_indexed 2023-09-18T21:05:14Z
_version_ 1777410889374236672

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