Phenolic content screening and in vitro antioxidant evaluation for radical scavenging activity of methanolic cladodes extract of Opuntia cochenillifera(L.) Mill
Opuntia cochenilifera is a tropical cactus from the family Cactaceae. It is commonly planted as an ornamental plant in Malaysia.The objectives of this study were to screen for phenolic content, optimize chromatographic system for thin layer chromatography (TLC) analysis and evaluate the antioxida...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Malaysian Pharmaceutical Society
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/45838/ http://irep.iium.edu.my/45838/ http://irep.iium.edu.my/45838/9/45838.pdf |
| Summary: | Opuntia cochenilifera is a tropical cactus from the family Cactaceae. It is commonly planted as
an ornamental plant in Malaysia.The objectives of this study were to screen for phenolic content,
optimize chromatographic system for thin layer chromatography (TLC) analysis and evaluate the
antioxidant activity of methanolic cladodes extract of Opuntia cochenillifera (L.) Mill. by using
DPPH radical scavenging method. Observation of the chromatograms under visible light showed
that the best compounds separation was achieved with the chromatographic system of
chloroform-methanol with ratio 90:10. The presence of phenolic compounds were detected by
staining the chromatogram with 10% methanolic ferric chloride reagent. At the highest
concentration tested (1 mg/mL), methanolic cladodes extract of Opuntia cochenillifera showed
41.5% DPPH radical inhibition in comparison with gallic acid, which showed 95.2% DPPH
radical inhibition. The results clearly indicated that the free radical scavenging activity of
methanolic cladodes extract of Opuntia cochenillifera was lower than the standard gallic acid.
The current study provides important baseline information to explore the potential use of
Opuntia cochenillifera as an ingredient in local nutraceutical and cosmeceutical products. |
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