Transdermal permeation mechanism of sodium deoxycholate aided nano-transfersomes by Differentail Sacnning Calorimetry (DSC)

Transfersomes are lipid based nano-vesicles made of phospholipid and surfactant. Their drug permeation mechanism through the skin has been attributed to the moisture seeking tendency (xerophobia) of the lipid vesicles followed by destabilization of lipid bi-layer in the stratum corneum (SC) by surfa...

Full description

Bibliographic Details
Main Authors:	Mahmood, Syed, Bakhtiar, M. Taher, Mandal, Uttam Kumar
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/45814/ http://irep.iium.edu.my/45814/ http://irep.iium.edu.my/45814/1/45814.pdf

id	iium-45814
recordtype	eprints
spelling	iium-458142018-06-21T03:11:58Z http://irep.iium.edu.my/45814/ Transdermal permeation mechanism of sodium deoxycholate aided nano-transfersomes by Differentail Sacnning Calorimetry (DSC) Mahmood, Syed Bakhtiar, M. Taher Mandal, Uttam Kumar RS Pharmacy and materia medica Transfersomes are lipid based nano-vesicles made of phospholipid and surfactant. Their drug permeation mechanism through the skin has been attributed to the moisture seeking tendency (xerophobia) of the lipid vesicles followed by destabilization of lipid bi-layer in the stratum corneum (SC) by surfactant1. However, structural changes of SC due to surfactant need further elucidation to know the specific role of that surfactant in doing so. The objective of this study was to evaluate drug permeation mechanism of raloxifene loaded nano-transfersomes containing sodium deoxycholate used as a surfactant. Phospholipon® 90G was used as a lipid composition in the nano-formulation2. Three different types of skin i.e. mice, guineapig, and rabbit were used for this study. The SC was detached from the rest of the skin layers with chemical treatment, thoroughly washed, and kept for drying in a vacuum desiccator. The SC samples were subjected to an ex-vivo permeation study of the transfersomal formulation for 8 hours. A control sample was prepared in a similar way, without any formulation treatment. A sample of the SC section was cut, sealed in aluminum hermetic pans and scanned using DSC at a scanning rate of 5°C per minute over the range of 25°C–125°C. The characteristic bi-layer lipid and keratin transition peaks found in the control SC samples were: 75°C, 78°C, and 95°C for mice; 820C, 900C, and 990C for guineapig; and 840C, 920C, 990C for rabbit3. However, upon treatment with sodium deoxycholate aided raloxifene nano-transfersomes, most of the peaks were shifted towards lower melting points and some of them were disappeared. This finding confirmed disruption of lipid bi-layer and denaturation of keratin in the SC layer of the investigated skin samples by nano-transfersomes with sodium deoxycholate. It also established the role of sodium deoxycholate in transdermal permeation of drug loaded nano-transfersomes formulation. 2015-11-12 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/45814/1/45814.pdf Mahmood, Syed and Bakhtiar, M. Taher and Mandal, Uttam Kumar (2015) Transdermal permeation mechanism of sodium deoxycholate aided nano-transfersomes by Differentail Sacnning Calorimetry (DSC). In: 4th International Conference on Pharmaceuticals, Nutraceuticals and Cosmetic Science (IPNaCs), 12th-13th Nov. 2015, Ramada Plaza, Malaka, Malaysia. (Unpublished) htpp://www.ipnacs.uitm.edu.my
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Mahmood, Syed Bakhtiar, M. Taher Mandal, Uttam Kumar Transdermal permeation mechanism of sodium deoxycholate aided nano-transfersomes by Differentail Sacnning Calorimetry (DSC)
description	Transfersomes are lipid based nano-vesicles made of phospholipid and surfactant. Their drug permeation mechanism through the skin has been attributed to the moisture seeking tendency (xerophobia) of the lipid vesicles followed by destabilization of lipid bi-layer in the stratum corneum (SC) by surfactant1. However, structural changes of SC due to surfactant need further elucidation to know the specific role of that surfactant in doing so. The objective of this study was to evaluate drug permeation mechanism of raloxifene loaded nano-transfersomes containing sodium deoxycholate used as a surfactant. Phospholipon® 90G was used as a lipid composition in the nano-formulation2. Three different types of skin i.e. mice, guineapig, and rabbit were used for this study. The SC was detached from the rest of the skin layers with chemical treatment, thoroughly washed, and kept for drying in a vacuum desiccator. The SC samples were subjected to an ex-vivo permeation study of the transfersomal formulation for 8 hours. A control sample was prepared in a similar way, without any formulation treatment. A sample of the SC section was cut, sealed in aluminum hermetic pans and scanned using DSC at a scanning rate of 5°C per minute over the range of 25°C–125°C. The characteristic bi-layer lipid and keratin transition peaks found in the control SC samples were: 75°C, 78°C, and 95°C for mice; 820C, 900C, and 990C for guineapig; and 840C, 920C, 990C for rabbit3. However, upon treatment with sodium deoxycholate aided raloxifene nano-transfersomes, most of the peaks were shifted towards lower melting points and some of them were disappeared. This finding confirmed disruption of lipid bi-layer and denaturation of keratin in the SC layer of the investigated skin samples by nano-transfersomes with sodium deoxycholate. It also established the role of sodium deoxycholate in transdermal permeation of drug loaded nano-transfersomes formulation.
format	Conference or Workshop Item
author	Mahmood, Syed Bakhtiar, M. Taher Mandal, Uttam Kumar
author_facet	Mahmood, Syed Bakhtiar, M. Taher Mandal, Uttam Kumar
author_sort	Mahmood, Syed
title	Transdermal permeation mechanism of sodium deoxycholate aided nano-transfersomes by Differentail Sacnning Calorimetry (DSC)
title_short	Transdermal permeation mechanism of sodium deoxycholate aided nano-transfersomes by Differentail Sacnning Calorimetry (DSC)
title_full	Transdermal permeation mechanism of sodium deoxycholate aided nano-transfersomes by Differentail Sacnning Calorimetry (DSC)
title_fullStr	Transdermal permeation mechanism of sodium deoxycholate aided nano-transfersomes by Differentail Sacnning Calorimetry (DSC)
title_full_unstemmed	Transdermal permeation mechanism of sodium deoxycholate aided nano-transfersomes by Differentail Sacnning Calorimetry (DSC)
title_sort	transdermal permeation mechanism of sodium deoxycholate aided nano-transfersomes by differentail sacnning calorimetry (dsc)
publishDate	2015
url	http://irep.iium.edu.my/45814/ http://irep.iium.edu.my/45814/ http://irep.iium.edu.my/45814/1/45814.pdf
first_indexed	2023-09-18T21:05:13Z
last_indexed	2023-09-18T21:05:13Z
_version_	1777410888240726016

Transdermal permeation mechanism of sodium deoxycholate aided nano-transfersomes by Differentail Sacnning Calorimetry (DSC)

Similar Items