Isolation and identification of rhizospheric bacteria associated with lemongrass for bioremediation of dibenzofuran contaminated soil

Contamination of soil and groundwater pollution is a severe problem, has been attracting considerable public attention over the last decades. The negative effects of pollutants on the environment and on the human health are diverse depend on the nature of the pollution. Thus, bioremediation offer a...

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Main Authors:	Sanusi, Najwa Husna, Phang, Ing Chia, Nordin, Noor Faizul Hadry
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/45709/ http://irep.iium.edu.my/45709/ http://irep.iium.edu.my/45709/5/45709.pdf

id	iium-45709
recordtype	eprints
spelling	iium-457092016-09-23T08:02:42Z http://irep.iium.edu.my/45709/ Isolation and identification of rhizospheric bacteria associated with lemongrass for bioremediation of dibenzofuran contaminated soil Sanusi, Najwa Husna Phang, Ing Chia Nordin, Noor Faizul Hadry Q Science (General) Contamination of soil and groundwater pollution is a severe problem, has been attracting considerable public attention over the last decades. The negative effects of pollutants on the environment and on the human health are diverse depend on the nature of the pollution. Thus, bioremediation offer a sustainable solution to the problem of contaminated environmental soil. With the demand for green and cleaner technology for remediation process, there is an increased interest in moving away from conventional technologies towards bioremediation technologies. Rhizospheric zone is a suitable place for harboring bacteria that are capable to utilize these chemical compounds which will be used either to facilitate growth of bacteria or the host plants. Identification of the specific microbial members should allow for better strategies to enhance biodegradation. This study aimed to isolate and identify the rhizospheric associated microbes of lemongrass (Cymbopogon citratus), a plant that commonly available in South East Asia. This probably is due to their ability to harbor large number of bacteria on their highly branched root systems. Total of 68 bacteria were isolated from the rhizospheric soil of lemongrass from 2 different unpolluted sites. Genomic DNA of the bacteria was successfully isolated and Polymerase Chain Reaction (PCR) amplification of 16S rRNA gene was achieved using 27F and 1492R primers. Purified PCR products from chosen samples were sent for sequencing. The identification of the bacteria was confirmed by sequence homology 16S rRNA gene studies. Several identified microbial strains isolated from rhizospheric zone, both Gram-negative (mainly the genera Enterobacter sp., Klebsiella sp., Aeromonas hydrophila, Chromobacterium sp., Pseudomonas fulva, Burkholderia sp., Citrobacter werkmanii, Stenotrophomas maltophilla, and Pantoea agglomerans) and Gram-positive bacteria (predominantly belonging to the genera Staphylococcus succinus and Staphylococcus saprophyticus). In degradation studies, the isolates showed the ability to utilize dibenzofuran up to 100 ppm with higher growth rate in utilizing the dibenzofuran. According to the result obtained, indigenous bacteria from rhizospheric soil of lemongrass were found to be able to degrade dibenzofuran hence can be utilized in bioremediation. 2015-08 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/45709/5/45709.pdf Sanusi, Najwa Husna and Phang, Ing Chia and Nordin, Noor Faizul Hadry (2015) Isolation and identification of rhizospheric bacteria associated with lemongrass for bioremediation of dibenzofuran contaminated soil. In: International Conference on Advancement in Science and Technology (iCAST 2015), 10-12 August 2015, Kuantan, Pahang. (Unpublished) http://www.iium.edu.my/icast/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Sanusi, Najwa Husna Phang, Ing Chia Nordin, Noor Faizul Hadry Isolation and identification of rhizospheric bacteria associated with lemongrass for bioremediation of dibenzofuran contaminated soil
description	Contamination of soil and groundwater pollution is a severe problem, has been attracting considerable public attention over the last decades. The negative effects of pollutants on the environment and on the human health are diverse depend on the nature of the pollution. Thus, bioremediation offer a sustainable solution to the problem of contaminated environmental soil. With the demand for green and cleaner technology for remediation process, there is an increased interest in moving away from conventional technologies towards bioremediation technologies. Rhizospheric zone is a suitable place for harboring bacteria that are capable to utilize these chemical compounds which will be used either to facilitate growth of bacteria or the host plants. Identification of the specific microbial members should allow for better strategies to enhance biodegradation. This study aimed to isolate and identify the rhizospheric associated microbes of lemongrass (Cymbopogon citratus), a plant that commonly available in South East Asia. This probably is due to their ability to harbor large number of bacteria on their highly branched root systems. Total of 68 bacteria were isolated from the rhizospheric soil of lemongrass from 2 different unpolluted sites. Genomic DNA of the bacteria was successfully isolated and Polymerase Chain Reaction (PCR) amplification of 16S rRNA gene was achieved using 27F and 1492R primers. Purified PCR products from chosen samples were sent for sequencing. The identification of the bacteria was confirmed by sequence homology 16S rRNA gene studies. Several identified microbial strains isolated from rhizospheric zone, both Gram-negative (mainly the genera Enterobacter sp., Klebsiella sp., Aeromonas hydrophila, Chromobacterium sp., Pseudomonas fulva, Burkholderia sp., Citrobacter werkmanii, Stenotrophomas maltophilla, and Pantoea agglomerans) and Gram-positive bacteria (predominantly belonging to the genera Staphylococcus succinus and Staphylococcus saprophyticus). In degradation studies, the isolates showed the ability to utilize dibenzofuran up to 100 ppm with higher growth rate in utilizing the dibenzofuran. According to the result obtained, indigenous bacteria from rhizospheric soil of lemongrass were found to be able to degrade dibenzofuran hence can be utilized in bioremediation.
format	Conference or Workshop Item
author	Sanusi, Najwa Husna Phang, Ing Chia Nordin, Noor Faizul Hadry
author_facet	Sanusi, Najwa Husna Phang, Ing Chia Nordin, Noor Faizul Hadry
author_sort	Sanusi, Najwa Husna
title	Isolation and identification of rhizospheric bacteria associated with lemongrass for bioremediation of dibenzofuran contaminated soil
title_short	Isolation and identification of rhizospheric bacteria associated with lemongrass for bioremediation of dibenzofuran contaminated soil
title_full	Isolation and identification of rhizospheric bacteria associated with lemongrass for bioremediation of dibenzofuran contaminated soil
title_fullStr	Isolation and identification of rhizospheric bacteria associated with lemongrass for bioremediation of dibenzofuran contaminated soil
title_full_unstemmed	Isolation and identification of rhizospheric bacteria associated with lemongrass for bioremediation of dibenzofuran contaminated soil
title_sort	isolation and identification of rhizospheric bacteria associated with lemongrass for bioremediation of dibenzofuran contaminated soil
publishDate	2015
url	http://irep.iium.edu.my/45709/ http://irep.iium.edu.my/45709/ http://irep.iium.edu.my/45709/5/45709.pdf
first_indexed	2023-09-18T21:05:03Z
last_indexed	2023-09-18T21:05:03Z
_version_	1777410877154131968

Isolation and identification of rhizospheric bacteria associated with lemongrass for bioremediation of dibenzofuran contaminated soil

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