Effect of salicylic acid on the transcript levels of SOD, POD and CAT in Nicotiana tabacum grown under Pb stress

Phytoremediation is a cost effective method which uses green plants to remediate Pb contaminants. Pb affects plant growth and development, and causes oxidative damage. Since plants are sessile organisms, compounds that can increase plant tolerance to Pb could enhance the efficiency of phytoremediat...

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Main Authors:	Abdul Halim, Nurul Izzah, Phang, Ing Chia
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/45682/ http://irep.iium.edu.my/45682/9/45682-new.pdf

id	iium-45682
recordtype	eprints
spelling	iium-456822016-09-27T08:02:35Z http://irep.iium.edu.my/45682/ Effect of salicylic acid on the transcript levels of SOD, POD and CAT in Nicotiana tabacum grown under Pb stress Abdul Halim, Nurul Izzah Phang, Ing Chia Q Science (General) Phytoremediation is a cost effective method which uses green plants to remediate Pb contaminants. Pb affects plant growth and development, and causes oxidative damage. Since plants are sessile organisms, compounds that can increase plant tolerance to Pb could enhance the efficiency of phytoremediation. In previous studies, SA was found to induce the level of antioxidant in plants, thus alleviating the harmful effect of excessive reactive oxygen species (ROS) level. It is likely that SA would reduce Pb-induced oxidative stress and increase the antioxidant capacity in Nicotiana tabacum. Superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) are antioxidative enzymes, which are commonly used as a marker for oxidative stress in plants. The present study aims to assess the effect of SA on the transcript levels of SOD, CAT and POD in N. tabacum grown under Pb treatments using a real time PCR. Firstly, N. tabacum was propagated and subjected to SA and Pb treatments. Then, RNA was isolated, cDNA was synthesized, and transcript levels of SOD, CAT and POD in N. tabacum were evaluated. The transcript levels of SOD, CAT and POD in SA pre-treated N. tabacum grown under Pb stress were compared to the controls and will be presented. The finding of this study improves our understanding on the effect of SA on the transcript level of SOD, CAT and POD in N. tabacum grown under Pb stress. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/45682/9/45682-new.pdf Abdul Halim, Nurul Izzah and Phang, Ing Chia (2014) Effect of salicylic acid on the transcript levels of SOD, POD and CAT in Nicotiana tabacum grown under Pb stress. In: International Conference on Advances in Plant Biochemistry and Biotechnology 2014 (APBB2014), 9-10 December 2014, Serdang, Selangor. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Abdul Halim, Nurul Izzah Phang, Ing Chia Effect of salicylic acid on the transcript levels of SOD, POD and CAT in Nicotiana tabacum grown under Pb stress
description	Phytoremediation is a cost effective method which uses green plants to remediate Pb contaminants. Pb affects plant growth and development, and causes oxidative damage. Since plants are sessile organisms, compounds that can increase plant tolerance to Pb could enhance the efficiency of phytoremediation. In previous studies, SA was found to induce the level of antioxidant in plants, thus alleviating the harmful effect of excessive reactive oxygen species (ROS) level. It is likely that SA would reduce Pb-induced oxidative stress and increase the antioxidant capacity in Nicotiana tabacum. Superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) are antioxidative enzymes, which are commonly used as a marker for oxidative stress in plants. The present study aims to assess the effect of SA on the transcript levels of SOD, CAT and POD in N. tabacum grown under Pb treatments using a real time PCR. Firstly, N. tabacum was propagated and subjected to SA and Pb treatments. Then, RNA was isolated, cDNA was synthesized, and transcript levels of SOD, CAT and POD in N. tabacum were evaluated. The transcript levels of SOD, CAT and POD in SA pre-treated N. tabacum grown under Pb stress were compared to the controls and will be presented. The finding of this study improves our understanding on the effect of SA on the transcript level of SOD, CAT and POD in N. tabacum grown under Pb stress.
format	Conference or Workshop Item
author	Abdul Halim, Nurul Izzah Phang, Ing Chia
author_facet	Abdul Halim, Nurul Izzah Phang, Ing Chia
author_sort	Abdul Halim, Nurul Izzah
title	Effect of salicylic acid on the transcript levels of SOD, POD and CAT in Nicotiana tabacum grown under Pb stress
title_short	Effect of salicylic acid on the transcript levels of SOD, POD and CAT in Nicotiana tabacum grown under Pb stress
title_full	Effect of salicylic acid on the transcript levels of SOD, POD and CAT in Nicotiana tabacum grown under Pb stress
title_fullStr	Effect of salicylic acid on the transcript levels of SOD, POD and CAT in Nicotiana tabacum grown under Pb stress
title_full_unstemmed	Effect of salicylic acid on the transcript levels of SOD, POD and CAT in Nicotiana tabacum grown under Pb stress
title_sort	effect of salicylic acid on the transcript levels of sod, pod and cat in nicotiana tabacum grown under pb stress
publishDate	2014
url	http://irep.iium.edu.my/45682/ http://irep.iium.edu.my/45682/9/45682-new.pdf
first_indexed	2023-09-18T21:05:00Z
last_indexed	2023-09-18T21:05:00Z
_version_	1777410874542129152

Effect of salicylic acid on the transcript levels of SOD, POD and CAT in Nicotiana tabacum grown under Pb stress

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