Transcript level of an andrographolide-biosynthesis gene (hmgr) of Andrographis paniculata subjected to salinity treatment

High demand of medicinal plants for traditional treatment and development of pharmaceutical products have attracted Malaysia to become a key herbal medicine producer for global and local usage. One of the Malaysian herbs which have a strong potential to be commercialized is Andrographis paniculata (...

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Main Authors:	Ismail, Nor Adibah, Phang, Ing Chia, Darnis, Deny Susanti
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	Q Science (General) QK Botany
Online Access:	http://irep.iium.edu.my/45680/ http://irep.iium.edu.my/45680/11/45680.pdf

id	iium-45680
recordtype	eprints
spelling	iium-456802016-09-27T08:41:08Z http://irep.iium.edu.my/45680/ Transcript level of an andrographolide-biosynthesis gene (hmgr) of Andrographis paniculata subjected to salinity treatment Ismail, Nor Adibah Phang, Ing Chia Darnis, Deny Susanti Q Science (General) QK Botany High demand of medicinal plants for traditional treatment and development of pharmaceutical products have attracted Malaysia to become a key herbal medicine producer for global and local usage. One of the Malaysian herbs which have a strong potential to be commercialized is Andrographis paniculata (King of bitters/Hempedu Bumi). This medicinal plant is well known for its therapeutical compound, diterpene lactones (andrographolide). Soil salinity is one of the major problems affecting agricultural sector worldwide because as salt accumulates in soil, plant productivity decreases. Generally, the higher the NaCl concentration, the greater the symptoms of salinity stress. These include browning of leaves and dehydration. One of the genes involved in biosynthesis of andrographolide in A. paniculata is 3-hydroxy-3-methylglutaryl-coenzyme A reductase (hmgr). Thus, the present study was conducted to investigate the transcript level of hmgr of A. paniculata grown under salinity condition. Primer pairs for hmgr have been designed, and the expression level of hmgr was quantified using a quantitative real time polymerase chain reaction (qPCR). First, RNA from A. paniculata leaves treated in different salinity concentrations including control was isolated and then converted to cDNA. From qPCR study, it is found that hmgr gene was up- regulated by 3.165-, 3.302-, and 3.473-fold in A. paniculata treated with 20.55, 41.1, and 66.75 mM NaCl, respectively, in comparison to the control. Salt may trigger the signalling network by up-regulating the hmgr expression level in plants. Andrographolide content is expected to correlate with the hmgr transcript level under saline condition. Determination of andrographolide content using HPLC is currently underway. Further details on the effect of salinity on the gene expression level of hmgr will be elucidated in the conference. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/45680/11/45680.pdf Ismail, Nor Adibah and Phang, Ing Chia and Darnis, Deny Susanti (2014) Transcript level of an andrographolide-biosynthesis gene (hmgr) of Andrographis paniculata subjected to salinity treatment. In: International Conference on Advances in Plant Biochemistry and Biotechnology 2014 (APBB2014), 9-10 December 2014, Serdang, Selangor. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) QK Botany
spellingShingle	Q Science (General) QK Botany Ismail, Nor Adibah Phang, Ing Chia Darnis, Deny Susanti Transcript level of an andrographolide-biosynthesis gene (hmgr) of Andrographis paniculata subjected to salinity treatment
description	High demand of medicinal plants for traditional treatment and development of pharmaceutical products have attracted Malaysia to become a key herbal medicine producer for global and local usage. One of the Malaysian herbs which have a strong potential to be commercialized is Andrographis paniculata (King of bitters/Hempedu Bumi). This medicinal plant is well known for its therapeutical compound, diterpene lactones (andrographolide). Soil salinity is one of the major problems affecting agricultural sector worldwide because as salt accumulates in soil, plant productivity decreases. Generally, the higher the NaCl concentration, the greater the symptoms of salinity stress. These include browning of leaves and dehydration. One of the genes involved in biosynthesis of andrographolide in A. paniculata is 3-hydroxy-3-methylglutaryl-coenzyme A reductase (hmgr). Thus, the present study was conducted to investigate the transcript level of hmgr of A. paniculata grown under salinity condition. Primer pairs for hmgr have been designed, and the expression level of hmgr was quantified using a quantitative real time polymerase chain reaction (qPCR). First, RNA from A. paniculata leaves treated in different salinity concentrations including control was isolated and then converted to cDNA. From qPCR study, it is found that hmgr gene was up- regulated by 3.165-, 3.302-, and 3.473-fold in A. paniculata treated with 20.55, 41.1, and 66.75 mM NaCl, respectively, in comparison to the control. Salt may trigger the signalling network by up-regulating the hmgr expression level in plants. Andrographolide content is expected to correlate with the hmgr transcript level under saline condition. Determination of andrographolide content using HPLC is currently underway. Further details on the effect of salinity on the gene expression level of hmgr will be elucidated in the conference.
format	Conference or Workshop Item
author	Ismail, Nor Adibah Phang, Ing Chia Darnis, Deny Susanti
author_facet	Ismail, Nor Adibah Phang, Ing Chia Darnis, Deny Susanti
author_sort	Ismail, Nor Adibah
title	Transcript level of an andrographolide-biosynthesis gene (hmgr) of Andrographis paniculata subjected to salinity treatment
title_short	Transcript level of an andrographolide-biosynthesis gene (hmgr) of Andrographis paniculata subjected to salinity treatment
title_full	Transcript level of an andrographolide-biosynthesis gene (hmgr) of Andrographis paniculata subjected to salinity treatment
title_fullStr	Transcript level of an andrographolide-biosynthesis gene (hmgr) of Andrographis paniculata subjected to salinity treatment
title_full_unstemmed	Transcript level of an andrographolide-biosynthesis gene (hmgr) of Andrographis paniculata subjected to salinity treatment
title_sort	transcript level of an andrographolide-biosynthesis gene (hmgr) of andrographis paniculata subjected to salinity treatment
publishDate	2014
url	http://irep.iium.edu.my/45680/ http://irep.iium.edu.my/45680/11/45680.pdf
first_indexed	2023-09-18T21:05:00Z
last_indexed	2023-09-18T21:05:00Z
_version_	1777410874262159360

Transcript level of an andrographolide-biosynthesis gene (hmgr) of Andrographis paniculata subjected to salinity treatment

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