Protein profile of Andrographis paniculata to salinity treatment

Protein profile of Andrographis paniculata to salinity treatment

Salinity is one of the environmental factors that limit growth, development and agricultural productivity in plants. Andrographis paniculata, a medicinal plant commonly used to treat diabetes and hypertension, is a salt-sensitive plant. In order to better understand salinity stress responses in A. p...

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Main Authors: Hossain, Md Sanower, Muhamad Bunnori, Noraslinda, Phang, Ing Chia
Format: Conference or Workshop Item
Language:English
Published: 2014
Subjects:
Q Science (General)
QK Botany
QP Physiology
Online Access:http://irep.iium.edu.my/45679/
http://irep.iium.edu.my/45679/12/45679.pdf
id iium-45679
recordtype eprints
spelling iium-456792016-09-27T08:27:51Z http://irep.iium.edu.my/45679/ Protein profile of Andrographis paniculata to salinity treatment Hossain, Md Sanower Muhamad Bunnori, Noraslinda Phang, Ing Chia Q Science (General) QK Botany QP Physiology Salinity is one of the environmental factors that limit growth, development and agricultural productivity in plants. Andrographis paniculata, a medicinal plant commonly used to treat diabetes and hypertension, is a salt-sensitive plant. In order to better understand salinity stress responses in A. paniculata, protein profiling using a two-dimensional polyacrylamide gel electrophoresis (2DGE) was performed. A. paniculata was grown and subjected to 0, 20.55, 41.1, 66.75 and 92.4 mM NaCl in a hydroponic culture. Total protein was extracted from the leaves of A. paniculata using chloroform-trichloroacetic acid-acetone method with 4-(2- hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer. From Bradford assay and SDS-PAGE, the extracted protein was found to be a good quantity and quality by comparing with standard protein (Bovine serum albumin) and protein marker, respectively. Protein extracts from A. paniculata treated with 41.1 mM NaCl and control were separated using 2DGE. A total of 153 and 166 protein spots were detected in 41.1 mM NaCl treatment and control, respectively. From PDQuest analysis, 91 protein spots were found to be differentially expressed. Among these protein spots, 41 were up-regulated, 37 were down-regulated and 13 became absent. Database search (i.e. SWISS-PROT) using predicted Mr and pI revealed that proteins that were up-regulated by more than 10-fold are Agamous-like MADS-box protein AGL97, NAP1-related protein 2, Putative two-component response regulator-like APRR8, 2 unknown proteins, and proteins that were down-regulated by more than 10-fold are Probable 26S proteasome complex subunit sem1-2, Peroxidase 22, Methylsterol monooxygenase 1-3, Ent-kaurene oxidase, chloroplastic, La-related protein 1C, 2 unknown proteins. These regulated proteins belong to several functional categories including stress and defense, energy, metabolism, protein synthesis and degradation, cell structure, and signal transduction. Identification of protein using MALDI-TOF is currently underway. The present work provides a new insight into salt stress responses in A. paniculata. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/45679/12/45679.pdf Hossain, Md Sanower and Muhamad Bunnori, Noraslinda and Phang, Ing Chia (2014) Protein profile of Andrographis paniculata to salinity treatment. In: International Conference on Advances in Plant Biochemistry and Biotechnology 2014 (APBB2014), 9-10 December 2014, Serdang, Selangor. (Unpublished)
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic Q Science (General)
QK Botany
QP Physiology
spellingShingle Q Science (General)
QK Botany
QP Physiology
Hossain, Md Sanower
Muhamad Bunnori, Noraslinda
Phang, Ing Chia
Protein profile of Andrographis paniculata to salinity treatment
description Salinity is one of the environmental factors that limit growth, development and agricultural productivity in plants. Andrographis paniculata, a medicinal plant commonly used to treat diabetes and hypertension, is a salt-sensitive plant. In order to better understand salinity stress responses in A. paniculata, protein profiling using a two-dimensional polyacrylamide gel electrophoresis (2DGE) was performed. A. paniculata was grown and subjected to 0, 20.55, 41.1, 66.75 and 92.4 mM NaCl in a hydroponic culture. Total protein was extracted from the leaves of A. paniculata using chloroform-trichloroacetic acid-acetone method with 4-(2- hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer. From Bradford assay and SDS-PAGE, the extracted protein was found to be a good quantity and quality by comparing with standard protein (Bovine serum albumin) and protein marker, respectively. Protein extracts from A. paniculata treated with 41.1 mM NaCl and control were separated using 2DGE. A total of 153 and 166 protein spots were detected in 41.1 mM NaCl treatment and control, respectively. From PDQuest analysis, 91 protein spots were found to be differentially expressed. Among these protein spots, 41 were up-regulated, 37 were down-regulated and 13 became absent. Database search (i.e. SWISS-PROT) using predicted Mr and pI revealed that proteins that were up-regulated by more than 10-fold are Agamous-like MADS-box protein AGL97, NAP1-related protein 2, Putative two-component response regulator-like APRR8, 2 unknown proteins, and proteins that were down-regulated by more than 10-fold are Probable 26S proteasome complex subunit sem1-2, Peroxidase 22, Methylsterol monooxygenase 1-3, Ent-kaurene oxidase, chloroplastic, La-related protein 1C, 2 unknown proteins. These regulated proteins belong to several functional categories including stress and defense, energy, metabolism, protein synthesis and degradation, cell structure, and signal transduction. Identification of protein using MALDI-TOF is currently underway. The present work provides a new insight into salt stress responses in A. paniculata.
format Conference or Workshop Item
author Hossain, Md Sanower
Muhamad Bunnori, Noraslinda
Phang, Ing Chia
author_facet Hossain, Md Sanower
Muhamad Bunnori, Noraslinda
Phang, Ing Chia
author_sort Hossain, Md Sanower
title Protein profile of Andrographis paniculata to salinity treatment
title_short Protein profile of Andrographis paniculata to salinity treatment
title_full Protein profile of Andrographis paniculata to salinity treatment
title_fullStr Protein profile of Andrographis paniculata to salinity treatment
title_full_unstemmed Protein profile of Andrographis paniculata to salinity treatment
title_sort protein profile of andrographis paniculata to salinity treatment
publishDate 2014
url http://irep.iium.edu.my/45679/
http://irep.iium.edu.my/45679/12/45679.pdf
first_indexed 2023-09-18T21:05:00Z
last_indexed 2023-09-18T21:05:00Z
_version_ 1777410874118504448

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