The effects of elicitors on lutein and zeaxanthin accumulation in in vitro cultures of Ruta angustifolia (L.) Pers.

In this study, the effects of selected elicitors, namely salicylic acid (SA) and methyl jasmonate (MeJa) on lutein and/or zeaxanthin accumulation in callus and shoot cultures of Ruta angustifolia (L.) Pers. were investigated. The carotenoid profile of intact leaves and in vitro cultures of R. angust...

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Main Authors:	Mohd Hassan, Norazian, Othman, Rashidi, Sulaiman, Shaida Fariza, Mohd Zaifuddin, Fatimah Azzahra
Format:	Monograph
Language:	English
Published:	2015
Subjects:	QD Chemistry SB Plant culture
Online Access:	http://irep.iium.edu.my/45379/ http://irep.iium.edu.my/45379/1/END_OF_PROJECT_REPORT-RACE12-001-0001.pdf

id	iium-45379
recordtype	eprints
spelling	iium-453792018-12-12T07:27:51Z http://irep.iium.edu.my/45379/ The effects of elicitors on lutein and zeaxanthin accumulation in in vitro cultures of Ruta angustifolia (L.) Pers. Mohd Hassan, Norazian Othman, Rashidi Sulaiman, Shaida Fariza Mohd Zaifuddin, Fatimah Azzahra QD Chemistry SB Plant culture In this study, the effects of selected elicitors, namely salicylic acid (SA) and methyl jasmonate (MeJa) on lutein and/or zeaxanthin accumulation in callus and shoot cultures of Ruta angustifolia (L.) Pers. were investigated. The carotenoid profile of intact leaves and in vitro cultures of R. angustifolia was initially investigated. The microshoot cultures were elicited with SA at concentrations of 0, 50 and 100 mg/ml whereas MeJa elicitation was studied at concentrations of 0, 20, 40 and 60 mg/mL. The cultures were incubated under 16 hours photoperiod at 25°C and were harvested periodically in 30 days interval for 90 days. Each sample was lyophyllized, extracted and analysed by reversed phase high-performance liquid chromatography (HPLC). The HPLC analysis showed that shoot cultures contained the highest total carotenoids content (14.70 ± 0.84 µg/mg) while lutein and β-carotene were the characteristics carotenoids that present in all R. angustifolia samples. SA elicitation increased accumulation of neoxanthin (15.88 ± 2.16 µg/mg DW), the specific carotenoid of the shoot cultures but decreased other carotenoids as compared to non-elicited cultures. MeJa was found to influence the culture growth and lutein production. The optimal elicitation strategy was MeJa elicitation on microshoot cultures with seven days exposure at 20 mg/mL and subsequent culture of 90 days, which resulted in the highest biomass and lutein production (141.07 ± 19.41 µg/mg DW), two times of non-elicited cultures. The carotenoids content in callus cultures was very low and both SA and MeJa elicitation gave no significant effect on carotenoids accumulation. No zeaxanthin was detected in all samples studied. In conclusion, the enhancement of lutein content in R. angustifolia can be achieved through MeJa elicitation on microshoot culture. 2015-10-22 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/45379/1/END_OF_PROJECT_REPORT-RACE12-001-0001.pdf Mohd Hassan, Norazian and Othman, Rashidi and Sulaiman, Shaida Fariza and Mohd Zaifuddin, Fatimah Azzahra (2015) The effects of elicitors on lutein and zeaxanthin accumulation in in vitro cultures of Ruta angustifolia (L.) Pers. Research Report. UNSPECIFIED. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry SB Plant culture
spellingShingle	QD Chemistry SB Plant culture Mohd Hassan, Norazian Othman, Rashidi Sulaiman, Shaida Fariza Mohd Zaifuddin, Fatimah Azzahra The effects of elicitors on lutein and zeaxanthin accumulation in in vitro cultures of Ruta angustifolia (L.) Pers.
description	In this study, the effects of selected elicitors, namely salicylic acid (SA) and methyl jasmonate (MeJa) on lutein and/or zeaxanthin accumulation in callus and shoot cultures of Ruta angustifolia (L.) Pers. were investigated. The carotenoid profile of intact leaves and in vitro cultures of R. angustifolia was initially investigated. The microshoot cultures were elicited with SA at concentrations of 0, 50 and 100 mg/ml whereas MeJa elicitation was studied at concentrations of 0, 20, 40 and 60 mg/mL. The cultures were incubated under 16 hours photoperiod at 25°C and were harvested periodically in 30 days interval for 90 days. Each sample was lyophyllized, extracted and analysed by reversed phase high-performance liquid chromatography (HPLC). The HPLC analysis showed that shoot cultures contained the highest total carotenoids content (14.70 ± 0.84 µg/mg) while lutein and β-carotene were the characteristics carotenoids that present in all R. angustifolia samples. SA elicitation increased accumulation of neoxanthin (15.88 ± 2.16 µg/mg DW), the specific carotenoid of the shoot cultures but decreased other carotenoids as compared to non-elicited cultures. MeJa was found to influence the culture growth and lutein production. The optimal elicitation strategy was MeJa elicitation on microshoot cultures with seven days exposure at 20 mg/mL and subsequent culture of 90 days, which resulted in the highest biomass and lutein production (141.07 ± 19.41 µg/mg DW), two times of non-elicited cultures. The carotenoids content in callus cultures was very low and both SA and MeJa elicitation gave no significant effect on carotenoids accumulation. No zeaxanthin was detected in all samples studied. In conclusion, the enhancement of lutein content in R. angustifolia can be achieved through MeJa elicitation on microshoot culture.
format	Monograph
author	Mohd Hassan, Norazian Othman, Rashidi Sulaiman, Shaida Fariza Mohd Zaifuddin, Fatimah Azzahra
author_facet	Mohd Hassan, Norazian Othman, Rashidi Sulaiman, Shaida Fariza Mohd Zaifuddin, Fatimah Azzahra
author_sort	Mohd Hassan, Norazian
title	The effects of elicitors on lutein and zeaxanthin accumulation in in vitro cultures of Ruta angustifolia (L.) Pers.
title_short	The effects of elicitors on lutein and zeaxanthin accumulation in in vitro cultures of Ruta angustifolia (L.) Pers.
title_full	The effects of elicitors on lutein and zeaxanthin accumulation in in vitro cultures of Ruta angustifolia (L.) Pers.
title_fullStr	The effects of elicitors on lutein and zeaxanthin accumulation in in vitro cultures of Ruta angustifolia (L.) Pers.
title_full_unstemmed	The effects of elicitors on lutein and zeaxanthin accumulation in in vitro cultures of Ruta angustifolia (L.) Pers.
title_sort	effects of elicitors on lutein and zeaxanthin accumulation in in vitro cultures of ruta angustifolia (l.) pers.
publishDate	2015
url	http://irep.iium.edu.my/45379/ http://irep.iium.edu.my/45379/1/END_OF_PROJECT_REPORT-RACE12-001-0001.pdf
first_indexed	2023-09-18T21:04:35Z
last_indexed	2023-09-18T21:04:35Z
_version_	1777410847950241792

The effects of elicitors on lutein and zeaxanthin accumulation in in vitro cultures of Ruta angustifolia (L.) Pers.

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