A model for arabidopsis thaliana cell suspension growth and sugar uptake kinetics

In this study, a kinetic model was developed for batch growth and sugar consumption by the cell suspension cultures of Arabidopsis thaliana (A. thaliana) Columbia (Col) ecotype cultivated in the continuous dark condition. The model included sucrose hydrolysis by the cell-wall invertase enzyme into g...

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Main Authors:	Mohamad Puad, Noor Illi, Abd-Karim, Khairiah, Mavituna, Ferda
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/45320/ http://irep.iium.edu.my/45320/ http://irep.iium.edu.my/45320/1/45320.pdf

id	iium-45320
recordtype	eprints
spelling	iium-453202018-03-28T01:41:34Z http://irep.iium.edu.my/45320/ A model for arabidopsis thaliana cell suspension growth and sugar uptake kinetics Mohamad Puad, Noor Illi Abd-Karim, Khairiah Mavituna, Ferda T Technology (General) In this study, a kinetic model was developed for batch growth and sugar consumption by the cell suspension cultures of Arabidopsis thaliana (A. thaliana) Columbia (Col) ecotype cultivated in the continuous dark condition. The model included sucrose hydrolysis by the cell-wall invertase enzyme into glucose and fructose and consumption of these hexoses at different rates to support cell growth. Several assumptions were made in order to simplify modelling of a complex system. The whole living cells in the culture were treated as one uniform biomass by assuming that all individual living cells are of the same size, shape, mass, age and physiology. In addition, temperature and all concentrations in the system were considered as uniform. All kinetic model parameters were obtained from a control experiment where Col cells were grown on 30 gL-1 sucrose as well as other independent experiments where Col cells were supplied with different concentrations and combinations of sugars. The model adequately described and predicted the growth and sugars profile of A. thaliana cells. 2015 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/45320/1/45320.pdf Mohamad Puad, Noor Illi and Abd-Karim, Khairiah and Mavituna, Ferda (2015) A model for arabidopsis thaliana cell suspension growth and sugar uptake kinetics. In: 28th Symposium of Malaysian Chemical Engineers (SOMChE 2015), 21st-22nd Oct. 2015, Palm Garden Hotel, IOI Resort Putrajaya. (Unpublished) http://www.ukm.my/somche2015/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) Mohamad Puad, Noor Illi Abd-Karim, Khairiah Mavituna, Ferda A model for arabidopsis thaliana cell suspension growth and sugar uptake kinetics
description	In this study, a kinetic model was developed for batch growth and sugar consumption by the cell suspension cultures of Arabidopsis thaliana (A. thaliana) Columbia (Col) ecotype cultivated in the continuous dark condition. The model included sucrose hydrolysis by the cell-wall invertase enzyme into glucose and fructose and consumption of these hexoses at different rates to support cell growth. Several assumptions were made in order to simplify modelling of a complex system. The whole living cells in the culture were treated as one uniform biomass by assuming that all individual living cells are of the same size, shape, mass, age and physiology. In addition, temperature and all concentrations in the system were considered as uniform. All kinetic model parameters were obtained from a control experiment where Col cells were grown on 30 gL-1 sucrose as well as other independent experiments where Col cells were supplied with different concentrations and combinations of sugars. The model adequately described and predicted the growth and sugars profile of A. thaliana cells.
format	Conference or Workshop Item
author	Mohamad Puad, Noor Illi Abd-Karim, Khairiah Mavituna, Ferda
author_facet	Mohamad Puad, Noor Illi Abd-Karim, Khairiah Mavituna, Ferda
author_sort	Mohamad Puad, Noor Illi
title	A model for arabidopsis thaliana cell suspension growth and sugar uptake kinetics
title_short	A model for arabidopsis thaliana cell suspension growth and sugar uptake kinetics
title_full	A model for arabidopsis thaliana cell suspension growth and sugar uptake kinetics
title_fullStr	A model for arabidopsis thaliana cell suspension growth and sugar uptake kinetics
title_full_unstemmed	A model for arabidopsis thaliana cell suspension growth and sugar uptake kinetics
title_sort	model for arabidopsis thaliana cell suspension growth and sugar uptake kinetics
publishDate	2015
url	http://irep.iium.edu.my/45320/ http://irep.iium.edu.my/45320/ http://irep.iium.edu.my/45320/1/45320.pdf
first_indexed	2023-09-18T21:04:31Z
last_indexed	2023-09-18T21:04:31Z
_version_	1777410843545174016

A model for arabidopsis thaliana cell suspension growth and sugar uptake kinetics

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