Microbial community structure during fluoranthene degradation in the presence of plants

Aims: To investigate bacterial and fungal community structure during degradation of varying concentrations (0–5000 mg kg �1) of the polycyclic aromatic hydrocarbon (PAH) fluoranthene in the presence or absence of tomato plants. Method and Results: Fluoranthene amended or unamended growing media...

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Main Authors:	Storey, Sean, Mohd Ashaari, Mardiana, McCabe, Gráinne, Harty, Mary, Dempsey, Rita, Doyle, Owen, Clipson, Nicholas, Doyle, Evelyn
Format:	Article
Language:	English
Published:	Wiley Online Library 2014
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/45311/ http://irep.iium.edu.my/45311/ http://irep.iium.edu.my/45311/ http://irep.iium.edu.my/45311/1/Microbial_community_structure_during_fluoranthene_degradation_in_the_presence_of_plants.pdf

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spelling	iium-453112017-11-08T07:29:42Z http://irep.iium.edu.my/45311/ Microbial community structure during fluoranthene degradation in the presence of plants Storey, Sean Mohd Ashaari, Mardiana McCabe, Gráinne Harty, Mary Dempsey, Rita Doyle, Owen Clipson, Nicholas Doyle, Evelyn QR Microbiology Aims: To investigate bacterial and fungal community structure during degradation of varying concentrations (0–5000 mg kg �1) of the polycyclic aromatic hydrocarbon (PAH) fluoranthene in the presence or absence of tomato plants. Method and Results: Fluoranthene amended or unamended growing media, with or without a plant, were incubated in pots in a glasshouse for 30 days. Fluoranthene concentration was quantified using GC-FID, while bacterial and fungal community structure was investigated using TRFLP and ARISA, respectively. The abundance of two ring hydroxylating dioxygenase genes was measured using qPCR. More fluoranthene was degraded in the presence of tomato plants at 500 and 5000 mg fluoranthene kg �1 (P < 0�001), and it had a toxic effect on plant growth. Bacterial and fungal community composition in the rhizosphere was significantly different from that in nonrhizospheric and unplanted samples. The influence of fluoranthene on bacterial communities overcame that of the plant root such that community composition in rhizosphere and planted nonrhizospheric samples was broadly similar when fluoranthene was present. PAH-RHDa-GP genes were more abundant than PAH-RHDa-GN genes in all treatments. PAH-RHDa-GN abundance was unaffected by either PAH concentration or growing medium type, while the abundance of PAH-RHDa-GP was greatest in the rhizosphere. Conclusions: Plants promoted microbial growth and diversity and led to increases in abundance of Gram-positive dioxygenase genes, correlated with increased degradation of fluoranthene. Significance and Impact of the Study: This work contributes to knowledge in the broad area of biodegradation and also provides useful information for the design of future bioremediation strategies. Wiley Online Library 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/45311/1/Microbial_community_structure_during_fluoranthene_degradation_in_the_presence_of_plants.pdf Storey, Sean and Mohd Ashaari, Mardiana and McCabe, Gráinne and Harty, Mary and Dempsey, Rita and Doyle, Owen and Clipson, Nicholas and Doyle, Evelyn (2014) Microbial community structure during fluoranthene degradation in the presence of plants. Journal of Applied Microbiology, 117. pp. 74-84. ISSN 1365-2672 (O) http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2672 doi:10.1111/jam.12518
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Storey, Sean Mohd Ashaari, Mardiana McCabe, Gráinne Harty, Mary Dempsey, Rita Doyle, Owen Clipson, Nicholas Doyle, Evelyn Microbial community structure during fluoranthene degradation in the presence of plants
description	Aims: To investigate bacterial and fungal community structure during degradation of varying concentrations (0–5000 mg kg �1) of the polycyclic aromatic hydrocarbon (PAH) fluoranthene in the presence or absence of tomato plants. Method and Results: Fluoranthene amended or unamended growing media, with or without a plant, were incubated in pots in a glasshouse for 30 days. Fluoranthene concentration was quantified using GC-FID, while bacterial and fungal community structure was investigated using TRFLP and ARISA, respectively. The abundance of two ring hydroxylating dioxygenase genes was measured using qPCR. More fluoranthene was degraded in the presence of tomato plants at 500 and 5000 mg fluoranthene kg �1 (P < 0�001), and it had a toxic effect on plant growth. Bacterial and fungal community composition in the rhizosphere was significantly different from that in nonrhizospheric and unplanted samples. The influence of fluoranthene on bacterial communities overcame that of the plant root such that community composition in rhizosphere and planted nonrhizospheric samples was broadly similar when fluoranthene was present. PAH-RHDa-GP genes were more abundant than PAH-RHDa-GN genes in all treatments. PAH-RHDa-GN abundance was unaffected by either PAH concentration or growing medium type, while the abundance of PAH-RHDa-GP was greatest in the rhizosphere. Conclusions: Plants promoted microbial growth and diversity and led to increases in abundance of Gram-positive dioxygenase genes, correlated with increased degradation of fluoranthene. Significance and Impact of the Study: This work contributes to knowledge in the broad area of biodegradation and also provides useful information for the design of future bioremediation strategies.
format	Article
author	Storey, Sean Mohd Ashaari, Mardiana McCabe, Gráinne Harty, Mary Dempsey, Rita Doyle, Owen Clipson, Nicholas Doyle, Evelyn
author_facet	Storey, Sean Mohd Ashaari, Mardiana McCabe, Gráinne Harty, Mary Dempsey, Rita Doyle, Owen Clipson, Nicholas Doyle, Evelyn
author_sort	Storey, Sean
title	Microbial community structure during fluoranthene degradation in the presence of plants
title_short	Microbial community structure during fluoranthene degradation in the presence of plants
title_full	Microbial community structure during fluoranthene degradation in the presence of plants
title_fullStr	Microbial community structure during fluoranthene degradation in the presence of plants
title_full_unstemmed	Microbial community structure during fluoranthene degradation in the presence of plants
title_sort	microbial community structure during fluoranthene degradation in the presence of plants
publisher	Wiley Online Library
publishDate	2014
url	http://irep.iium.edu.my/45311/ http://irep.iium.edu.my/45311/ http://irep.iium.edu.my/45311/ http://irep.iium.edu.my/45311/1/Microbial_community_structure_during_fluoranthene_degradation_in_the_presence_of_plants.pdf
first_indexed	2023-09-18T21:04:30Z
last_indexed	2023-09-18T21:04:30Z
_version_	1777410843254718464

Microbial community structure during fluoranthene degradation in the presence of plants

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