Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures

A stoichiometric model was developed for the application of metabolic flux balance analysis (FBA) which consisted of the primary metabolic network of Arabidopsis thaliana (A. thaliana). The basic metabolic model involved 149 metabolites and 178 reactions that represent the main carbon metabolism tow...

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Main Authors:	Mohamad Puad, Noor Illi, Mavituna, Ferda
Format:	Conference or Workshop Item
Language:	English English
Published:	2015
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/44876/ http://irep.iium.edu.my/44876/ http://irep.iium.edu.my/44876/1/44876.pdf http://irep.iium.edu.my/44876/4/IN-FOO-002.pdf

id	iium-44876
recordtype	eprints
spelling	iium-448762016-05-24T00:40:01Z http://irep.iium.edu.my/44876/ Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures Mohamad Puad, Noor Illi Mavituna, Ferda T Technology (General) A stoichiometric model was developed for the application of metabolic flux balance analysis (FBA) which consisted of the primary metabolic network of Arabidopsis thaliana (A. thaliana). The basic metabolic model involved 149 metabolites and 178 reactions that represent the main carbon metabolism towards the formation of biomass. Assuming pseudo-steady state, mass balances were performed for each metabolite and the resulting equations were solved using linear optimization. The optimal metabolic flux distribution was determined in General Algebraic Modeling System (GAMS) 23.0 software by setting up the objective function as the maximization of biomass formation rate and using the constraints from batch experiments of A. thaliana suspended cell cultures. The results gave the theoretical maximum biological capacity of the cells to grow for a given specific uptake rate of sugar. The model proved to be a good starting point for more extended version of metabolism specifically for secondary metabolite production. Addition or extension of the model is feasible. 2015-09-24 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/44876/1/44876.pdf application/pdf en http://irep.iium.edu.my/44876/4/IN-FOO-002.pdf Mohamad Puad, Noor Illi and Mavituna, Ferda (2015) Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures. In: The 22nd Regional Symposium on Chemical Engineering (RSCE 2015), 24th-25th Sept. 2015, Bangkok, Thailand. http://www.rsce2015.org/page/1
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	T Technology (General)
spellingShingle	T Technology (General) Mohamad Puad, Noor Illi Mavituna, Ferda Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures
description	A stoichiometric model was developed for the application of metabolic flux balance analysis (FBA) which consisted of the primary metabolic network of Arabidopsis thaliana (A. thaliana). The basic metabolic model involved 149 metabolites and 178 reactions that represent the main carbon metabolism towards the formation of biomass. Assuming pseudo-steady state, mass balances were performed for each metabolite and the resulting equations were solved using linear optimization. The optimal metabolic flux distribution was determined in General Algebraic Modeling System (GAMS) 23.0 software by setting up the objective function as the maximization of biomass formation rate and using the constraints from batch experiments of A. thaliana suspended cell cultures. The results gave the theoretical maximum biological capacity of the cells to grow for a given specific uptake rate of sugar. The model proved to be a good starting point for more extended version of metabolism specifically for secondary metabolite production. Addition or extension of the model is feasible.
format	Conference or Workshop Item
author	Mohamad Puad, Noor Illi Mavituna, Ferda
author_facet	Mohamad Puad, Noor Illi Mavituna, Ferda
author_sort	Mohamad Puad, Noor Illi
title	Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures
title_short	Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures
title_full	Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures
title_fullStr	Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures
title_full_unstemmed	Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures
title_sort	metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures
publishDate	2015
url	http://irep.iium.edu.my/44876/ http://irep.iium.edu.my/44876/ http://irep.iium.edu.my/44876/1/44876.pdf http://irep.iium.edu.my/44876/4/IN-FOO-002.pdf
first_indexed	2023-09-18T21:03:49Z
last_indexed	2023-09-18T21:03:49Z
_version_	1777410799931752448

Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures

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