Spermidine/spermine N1-acetyltransferase gene expression in A549 cells treated with pomegranate juice

Spermidine/spermine N1-acetyltransferase gene expression in A549 cells treated with pomegranate juice

Introduction: Intracellular polyamines have an important role in cell proliferation and death pathway. Targeting polyamine metabolic pathway is therefore a potential intervention in cancers. Either downregulation of polyamines synthesis or induction of polyamine catabolism both had been shown to cau...

Full description

Bibliographic Details
Main Authors: Nik Abdul Malek, Nik Nurasyikin, Abdul Ghani, Radiah
Format: Conference or Workshop Item
Language:English
Published: 2015
Subjects:
RB Pathology
Online Access:http://irep.iium.edu.my/44687/
http://irep.iium.edu.my/44687/10/13.pdf
id iium-44687
recordtype eprints
spelling iium-446872015-09-11T07:24:37Z http://irep.iium.edu.my/44687/ Spermidine/spermine N1-acetyltransferase gene expression in A549 cells treated with pomegranate juice Nik Abdul Malek, Nik Nurasyikin Abdul Ghani, Radiah RB Pathology Introduction: Intracellular polyamines have an important role in cell proliferation and death pathway. Targeting polyamine metabolic pathway is therefore a potential intervention in cancers. Either downregulation of polyamines synthesis or induction of polyamine catabolism both had been shown to cause depletion of intracellular polyamines. Objective: This study was aimed to investigate the expression of genes involved in polyamine catabolism, spermidine/spermine N1-acetyltransferase (SSAT)in human lung carcinoma A549 cells after treated with pomegranate juice. Methods: A549 cells were grown in DMEM supplemented with 10% (v/v) fetal bovine serum and 1% (v/v) penicillin-streptomycin. For experiment, cells were seeded at 1×104 cell/ml and grown for 48 hour prior to treatment. A549 cells were treated with 2% pomegranate juice for various exposure times between 0 to 48 hours. RNA was isolated using Trizol reagent and the purity of RNA was measured using NanoDrop spectrophotometer. The assays were analyzed using two steps quantitative real-time PCR by using Bio-Rad CFX96 Real-Time PCR Systems. The assays were normalized against two reference genes which are GAPDH and β-actin. Results: In this study, pomegranate juice did not induce the expression of SSAT. After 0, 6, 12, and 24 hour exposure, the expression of SSAT remains unchanged (p>0.05) compared to control. At 48 hour exposure, the expression of SSAT also remains unchanged although the relative normalized expression was lowered compared to control (p<0.05). Conclusion: To sum up, pomegranate juice had been shown to inhibit A549 cell lines proliferation although it did not induce the expression of SSAT. Therefore, it can be deduced that induction of SSAT is not involve in the death pathway in pomegranate juice treated human lung carcinoma A549 cell lines. Keywords: Polyamine catabolism, Pomegranate juice, Spermidine/spermine N1-acetyltransferase 2015-09-05 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/44687/10/13.pdf Nik Abdul Malek, Nik Nurasyikin and Abdul Ghani, Radiah (2015) Spermidine/spermine N1-acetyltransferase gene expression in A549 cells treated with pomegranate juice. In: International Symposium on Allied Health Sciences, 5th - 6 th September 2015, Kota Bharu, Kelantan. (Unpublished)
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RB Pathology
spellingShingle RB Pathology
Nik Abdul Malek, Nik Nurasyikin
Abdul Ghani, Radiah
Spermidine/spermine N1-acetyltransferase gene expression in A549 cells treated with pomegranate juice
description Introduction: Intracellular polyamines have an important role in cell proliferation and death pathway. Targeting polyamine metabolic pathway is therefore a potential intervention in cancers. Either downregulation of polyamines synthesis or induction of polyamine catabolism both had been shown to cause depletion of intracellular polyamines. Objective: This study was aimed to investigate the expression of genes involved in polyamine catabolism, spermidine/spermine N1-acetyltransferase (SSAT)in human lung carcinoma A549 cells after treated with pomegranate juice. Methods: A549 cells were grown in DMEM supplemented with 10% (v/v) fetal bovine serum and 1% (v/v) penicillin-streptomycin. For experiment, cells were seeded at 1×104 cell/ml and grown for 48 hour prior to treatment. A549 cells were treated with 2% pomegranate juice for various exposure times between 0 to 48 hours. RNA was isolated using Trizol reagent and the purity of RNA was measured using NanoDrop spectrophotometer. The assays were analyzed using two steps quantitative real-time PCR by using Bio-Rad CFX96 Real-Time PCR Systems. The assays were normalized against two reference genes which are GAPDH and β-actin. Results: In this study, pomegranate juice did not induce the expression of SSAT. After 0, 6, 12, and 24 hour exposure, the expression of SSAT remains unchanged (p>0.05) compared to control. At 48 hour exposure, the expression of SSAT also remains unchanged although the relative normalized expression was lowered compared to control (p<0.05). Conclusion: To sum up, pomegranate juice had been shown to inhibit A549 cell lines proliferation although it did not induce the expression of SSAT. Therefore, it can be deduced that induction of SSAT is not involve in the death pathway in pomegranate juice treated human lung carcinoma A549 cell lines. Keywords: Polyamine catabolism, Pomegranate juice, Spermidine/spermine N1-acetyltransferase
format Conference or Workshop Item
author Nik Abdul Malek, Nik Nurasyikin
Abdul Ghani, Radiah
author_facet Nik Abdul Malek, Nik Nurasyikin
Abdul Ghani, Radiah
author_sort Nik Abdul Malek, Nik Nurasyikin
title Spermidine/spermine N1-acetyltransferase gene expression in A549 cells treated with pomegranate juice
title_short Spermidine/spermine N1-acetyltransferase gene expression in A549 cells treated with pomegranate juice
title_full Spermidine/spermine N1-acetyltransferase gene expression in A549 cells treated with pomegranate juice
title_fullStr Spermidine/spermine N1-acetyltransferase gene expression in A549 cells treated with pomegranate juice
title_full_unstemmed Spermidine/spermine N1-acetyltransferase gene expression in A549 cells treated with pomegranate juice
title_sort spermidine/spermine n1-acetyltransferase gene expression in a549 cells treated with pomegranate juice
publishDate 2015
url http://irep.iium.edu.my/44687/
http://irep.iium.edu.my/44687/10/13.pdf
first_indexed 2023-09-18T21:03:31Z
last_indexed 2023-09-18T21:03:31Z
_version_ 1777410781287022592

Similar Items