Selective isolation of actinomycetes from mangrove forest of Pahang, Malaysia

Actinomycetes are widely distributed in nature and are important industrially and pharmaceutically. They are renowned for their unique capabilities to produce biologically active metabolites. Poorly studied marine ecosystem, particularly mangrove offers a promising natural resource of new actinomyce...

Full description

Bibliographic Details
Main Authors:	Abdul Malek, Nurfathiah, Chowdhury, Ahmed Jalal Khan, Zainuddin, Zarina, Zainal Abidin, Zaima Azira
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/44637/ http://irep.iium.edu.my/44637/ http://irep.iium.edu.my/44637/1/44637.pdf

id	iium-44637
recordtype	eprints
spelling	iium-446372018-06-29T02:59:17Z http://irep.iium.edu.my/44637/ Selective isolation of actinomycetes from mangrove forest of Pahang, Malaysia Abdul Malek, Nurfathiah Chowdhury, Ahmed Jalal Khan Zainuddin, Zarina Zainal Abidin, Zaima Azira QR Microbiology Actinomycetes are widely distributed in nature and are important industrially and pharmaceutically. They are renowned for their unique capabilities to produce biologically active metabolites. Poorly studied marine ecosystem, particularly mangrove offers a promising natural resource of new actinomycetes due to their high species diversity and novel biosynthetic capabilities. This study aims to assess the diversity of actinomycetes from mangrove forest of Pahang, Malaysia. Various selective isolation media and pre-treatments were employed for maximum isolation of mangrove actinomycetes. Sediment samples were collected from seven stations and air dried prior isolation. The samples were then pre-treated using wet heat and dry heat method followed by dilution plating on 8 media – starch-yeast extract, yeast extract-malt extract agar, oatmeal agar, inorganic salt-starch agar, starch casein agar, glucose asparagine agar, actinomycete isolation agar and marine agar. A total of 4850 actinomycete isolates had been successfully recovered. It was observed that inorganic-salt starch agar (ISP4) displayed the highest percentage of actinomycetes recovery (31.7 %), followed by actinomycete isolation agar (AIA) (24.1 %). Starch-yeast extract (SYE) and starch casein agar (SCA) were found to be more efficient in isolating wide variety of actinomycetes based on their morphological appearance. Moreover, both SYE and SCA were found to be more suitable for the isolation of non-Streptomyces isolates. Besides that, it was observed that wet heat pre-treatment was more effective in isolating mangrove actinomycetes as 81.4 % of total isolates were yielded using this moist-heating technique. On the other hand, dry eat treatment was better in the enumeration of spore-forming actinomycetes and removal of unwanted microorganisms, despite the lower number of isolates recovered. Of both pre-treatments, station 1 which is located within the deep mangrove forest area farthest from the coastal line recorded the highest recovery of actinomycetes (23.7 %), with Streptomyces and Micromonospora-like isolates being the most predominant observed from all sampling sites. The presence of relatively large number of isolates suggests that Pahang mangrove forest is a potentially rich source of actinomycetes. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/44637/1/44637.pdf Abdul Malek, Nurfathiah and Chowdhury, Ahmed Jalal Khan and Zainuddin, Zarina and Zainal Abidin, Zaima Azira (2014) Selective isolation of actinomycetes from mangrove forest of Pahang, Malaysia. In: International Conference on Agriculture, Biology and Environmental Sciences (ICABES-14), 8th-9th Dec. 2014, Bali, Indonesia. http://iaast.org/siteadmin/upload/8973A1214007.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Abdul Malek, Nurfathiah Chowdhury, Ahmed Jalal Khan Zainuddin, Zarina Zainal Abidin, Zaima Azira Selective isolation of actinomycetes from mangrove forest of Pahang, Malaysia
description	Actinomycetes are widely distributed in nature and are important industrially and pharmaceutically. They are renowned for their unique capabilities to produce biologically active metabolites. Poorly studied marine ecosystem, particularly mangrove offers a promising natural resource of new actinomycetes due to their high species diversity and novel biosynthetic capabilities. This study aims to assess the diversity of actinomycetes from mangrove forest of Pahang, Malaysia. Various selective isolation media and pre-treatments were employed for maximum isolation of mangrove actinomycetes. Sediment samples were collected from seven stations and air dried prior isolation. The samples were then pre-treated using wet heat and dry heat method followed by dilution plating on 8 media – starch-yeast extract, yeast extract-malt extract agar, oatmeal agar, inorganic salt-starch agar, starch casein agar, glucose asparagine agar, actinomycete isolation agar and marine agar. A total of 4850 actinomycete isolates had been successfully recovered. It was observed that inorganic-salt starch agar (ISP4) displayed the highest percentage of actinomycetes recovery (31.7 %), followed by actinomycete isolation agar (AIA) (24.1 %). Starch-yeast extract (SYE) and starch casein agar (SCA) were found to be more efficient in isolating wide variety of actinomycetes based on their morphological appearance. Moreover, both SYE and SCA were found to be more suitable for the isolation of non-Streptomyces isolates. Besides that, it was observed that wet heat pre-treatment was more effective in isolating mangrove actinomycetes as 81.4 % of total isolates were yielded using this moist-heating technique. On the other hand, dry eat treatment was better in the enumeration of spore-forming actinomycetes and removal of unwanted microorganisms, despite the lower number of isolates recovered. Of both pre-treatments, station 1 which is located within the deep mangrove forest area farthest from the coastal line recorded the highest recovery of actinomycetes (23.7 %), with Streptomyces and Micromonospora-like isolates being the most predominant observed from all sampling sites. The presence of relatively large number of isolates suggests that Pahang mangrove forest is a potentially rich source of actinomycetes.
format	Conference or Workshop Item
author	Abdul Malek, Nurfathiah Chowdhury, Ahmed Jalal Khan Zainuddin, Zarina Zainal Abidin, Zaima Azira
author_facet	Abdul Malek, Nurfathiah Chowdhury, Ahmed Jalal Khan Zainuddin, Zarina Zainal Abidin, Zaima Azira
author_sort	Abdul Malek, Nurfathiah
title	Selective isolation of actinomycetes from mangrove forest of Pahang, Malaysia
title_short	Selective isolation of actinomycetes from mangrove forest of Pahang, Malaysia
title_full	Selective isolation of actinomycetes from mangrove forest of Pahang, Malaysia
title_fullStr	Selective isolation of actinomycetes from mangrove forest of Pahang, Malaysia
title_full_unstemmed	Selective isolation of actinomycetes from mangrove forest of Pahang, Malaysia
title_sort	selective isolation of actinomycetes from mangrove forest of pahang, malaysia
publishDate	2014
url	http://irep.iium.edu.my/44637/ http://irep.iium.edu.my/44637/ http://irep.iium.edu.my/44637/1/44637.pdf
first_indexed	2023-09-18T21:03:26Z
last_indexed	2023-09-18T21:03:26Z
_version_	1777410776145854464

Selective isolation of actinomycetes from mangrove forest of Pahang, Malaysia

Similar Items