Biohydrogen production by bacteria using sago waste and its metabolic flux balancing

Hydrogen has been recognized as one of the potential alternative energy sources which can be used for many different purposes. It can be produced from microbial cultivation with waste organic materials. However, the amount of biohydrogen produced is still at an unsatisfactorily level. Besides, raw m...

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Main Authors:	Mohamad Puad, Noor Illi, Tajor Amar, Tajul Syaffiq
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/44634/ http://irep.iium.edu.my/44634/13/bio.pdf

id	iium-44634
recordtype	eprints
spelling	iium-446342015-09-11T01:38:57Z http://irep.iium.edu.my/44634/ Biohydrogen production by bacteria using sago waste and its metabolic flux balancing Mohamad Puad, Noor Illi Tajor Amar, Tajul Syaffiq T Technology (General) Hydrogen has been recognized as one of the potential alternative energy sources which can be used for many different purposes. It can be produced from microbial cultivation with waste organic materials. However, the amount of biohydrogen produced is still at an unsatisfactorily level. Besides, raw material cost is another concern for making the whole process economically feasible. This study aimed to use Flux Balance Analysis (FBA) to investigate and understand the production of biohydrogen using microbial cultures with sago waste as the substrate. FBA is a powerful quantitative approach to recognize and determine the specific pathways or branch reactions that influence the most in a specific metabolite formation. A stoichiometric balance metabolic model has been reconstructed based on the latest published articles, biochemistry books and online resources (Kyoto Encyclopedia of Genes and Genome (KEGG) and MetaCyc) using Escherichia coli (E.coli) as the model. This model consists of 206 metabolic reactions with 144 metabolites towards the production of biomass and biohydrogen. The carbon metabolic pathways include glycolysis/gluconeogenesis, pentose phosphate pathway (PPP), pyruvate metabolism, tricarboxylic acid (TCA) cycle and electron transport. A series of batch experiments involving E. coli and Enterobacter aerogenes (E. aerogenes) were carried out and the experimental data such as sugar uptake and specific growth rates as well as the biomass composition (proteins, RNA, DNA and lipids) were used as the constraints for the model. The objective function was set as the maximization of hydrogen yield in order to solve the unknown fluxes using Linear Programming (LP) method in General Algebraic Modelling System (GAMS) software. The FBA results showed the pathways that are involved in the biohydrogen production and the metabolites that will affect the most the yield of biohydrogen in both hydrogen-producing bacteria. In addition, further in silico analysis was done on the metabolic mdoel such as deletion of certain metabolites to investigate their effect on biohydrogen yield. 2015-09-01 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/44634/13/bio.pdf Mohamad Puad, Noor Illi and Tajor Amar, Tajul Syaffiq (2015) Biohydrogen production by bacteria using sago waste and its metabolic flux balancing. In: International Conference on Fuel Cell and Hydrogen Technology 2015, 1-3 September 2015, Kuala Lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) Mohamad Puad, Noor Illi Tajor Amar, Tajul Syaffiq Biohydrogen production by bacteria using sago waste and its metabolic flux balancing
description	Hydrogen has been recognized as one of the potential alternative energy sources which can be used for many different purposes. It can be produced from microbial cultivation with waste organic materials. However, the amount of biohydrogen produced is still at an unsatisfactorily level. Besides, raw material cost is another concern for making the whole process economically feasible. This study aimed to use Flux Balance Analysis (FBA) to investigate and understand the production of biohydrogen using microbial cultures with sago waste as the substrate. FBA is a powerful quantitative approach to recognize and determine the specific pathways or branch reactions that influence the most in a specific metabolite formation. A stoichiometric balance metabolic model has been reconstructed based on the latest published articles, biochemistry books and online resources (Kyoto Encyclopedia of Genes and Genome (KEGG) and MetaCyc) using Escherichia coli (E.coli) as the model. This model consists of 206 metabolic reactions with 144 metabolites towards the production of biomass and biohydrogen. The carbon metabolic pathways include glycolysis/gluconeogenesis, pentose phosphate pathway (PPP), pyruvate metabolism, tricarboxylic acid (TCA) cycle and electron transport. A series of batch experiments involving E. coli and Enterobacter aerogenes (E. aerogenes) were carried out and the experimental data such as sugar uptake and specific growth rates as well as the biomass composition (proteins, RNA, DNA and lipids) were used as the constraints for the model. The objective function was set as the maximization of hydrogen yield in order to solve the unknown fluxes using Linear Programming (LP) method in General Algebraic Modelling System (GAMS) software. The FBA results showed the pathways that are involved in the biohydrogen production and the metabolites that will affect the most the yield of biohydrogen in both hydrogen-producing bacteria. In addition, further in silico analysis was done on the metabolic mdoel such as deletion of certain metabolites to investigate their effect on biohydrogen yield.
format	Conference or Workshop Item
author	Mohamad Puad, Noor Illi Tajor Amar, Tajul Syaffiq
author_facet	Mohamad Puad, Noor Illi Tajor Amar, Tajul Syaffiq
author_sort	Mohamad Puad, Noor Illi
title	Biohydrogen production by bacteria using sago waste and its metabolic flux balancing
title_short	Biohydrogen production by bacteria using sago waste and its metabolic flux balancing
title_full	Biohydrogen production by bacteria using sago waste and its metabolic flux balancing
title_fullStr	Biohydrogen production by bacteria using sago waste and its metabolic flux balancing
title_full_unstemmed	Biohydrogen production by bacteria using sago waste and its metabolic flux balancing
title_sort	biohydrogen production by bacteria using sago waste and its metabolic flux balancing
publishDate	2015
url	http://irep.iium.edu.my/44634/ http://irep.iium.edu.my/44634/13/bio.pdf
first_indexed	2023-09-18T21:03:26Z
last_indexed	2023-09-18T21:03:26Z
_version_	1777410775850156032

Biohydrogen production by bacteria using sago waste and its metabolic flux balancing

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