Identification of alpha-glucosidase inhibitors in Cosmos caudatus leaves using GC-MS based metabolomics
Cosmos caudatus (“Ulam Raja” in Malaysia) is a plant used in Malaysia to enhance vitality and has been studied for its antidiabetic activity. This study focused on the evaluation of the α-glucosidase inhibitory activity of different ethanolic extracts of C. caudatus. Six series of samples extracted...
| Main Authors: | , , , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/44594/ http://irep.iium.edu.my/44594/ http://irep.iium.edu.my/44594/1/44594.pdf |
| Summary: | Cosmos caudatus (“Ulam Raja” in Malaysia) is a plant used in Malaysia to enhance vitality and has been studied for its antidiabetic activity. This study focused on the evaluation of the α-glucosidase inhibitory activity of different ethanolic extracts of C. caudatus. Six series of samples extracted with water, 20%, 40%, 60%, 80%, and 100% ethanol were employed. Gas chromatography-mass spectrometry (GC-MS) and multivariate data analysis was used to correlate bioactivity of different extracts to different metabolite profiles of C. caudatus. The obtained loading scores indicated a distinct and remarkable separation into 6 clusters, which were indicative of the 6 different quality of samples. GCMS can be integrated with multivariate data analysis to identify compounds that inhibit α-glucosidase activity. Through this approach several chemical makers e.g. catechin, α-linolenic acid, α-D-glucopyranoside, and vitamin E compounds were identified as an α-glucosidase inhibitors of this herb. |
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