Assessment of reactive oxygen species status and neuroprotection by vitamin e in chronic cerebral ypoperfusion-induced neurodegeneration in rats

A persistent reduction in regional cerebral blood flow (CBF) compromises memory and cognitive functions in the elderly leading to neurological illnesses. To unravel the neuropathological consequences of a reduced CBF a similar condition has been created in rats by common carotid artery occlusion (2...

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Main Authors:	Sayeed, Sayyada, Saxena, Anil Kumar, Talib, Norlelawati A., Azzubaidi, Marwan Saad
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	R Medicine (General) RM214 Diet therapy. Diet and dietectics in disease
Online Access:	http://irep.iium.edu.my/44589/ http://irep.iium.edu.my/44589/ http://irep.iium.edu.my/44589/1/44589.pdf

id	iium-44589
recordtype	eprints
spelling	iium-445892016-05-23T04:18:31Z http://irep.iium.edu.my/44589/ Assessment of reactive oxygen species status and neuroprotection by vitamin e in chronic cerebral ypoperfusion-induced neurodegeneration in rats Sayeed, Sayyada Saxena, Anil Kumar Talib, Norlelawati A. Azzubaidi, Marwan Saad R Medicine (General) RM214 Diet therapy. Diet and dietectics in disease A persistent reduction in regional cerebral blood flow (CBF) compromises memory and cognitive functions in the elderly leading to neurological illnesses. To unravel the neuropathological consequences of a reduced CBF a similar condition has been created in rats by common carotid artery occlusion (2 vessel occlusion, 2VO). Since oxidative stress, leading to neuronal death, is a major risk factor in neurodegenerative disorders, the present study was designed to assess the neuroprotective role of Vitamin E (Vit E) in chronic cerebral hypoperfusion induced-neurodegeneration. After acclimatization, twenty four Sprague Dawley rats weighing 200-250 g were equally divided into three groups. Group A – sham control, Group B–2VO, and Group C–2VO-E (treated daily with Vit E, 100 mg/kg, orally following 2VO). On the 8th week, all the rats were euthanized and the hippocampi were isolated. Viable neuronal cell count in the hippocampal CA-1 region was estimated. The Isoprostane F2 (Iso-F2) levels were also measured in the brain homogenates to quantify the oxidative stress levels. There was significant difference in neuronal cell death in 2VO group as compared to sham group. In 2VO-E rats, the viable neuronal cell count of the hippocampal CA-1 region was significantly higher (p<0.05) as compared to the 2VO group. Moreover, Iso-F2 levels in 2VO group was significantly higher (p<0.05) as compared to 2VO-E group, implying high oxidative stress in 2VO group and reduction of oxidative stress levels in 2VO-E group. This study clearly demonstrates the effectiveness of Vit E as a neuroprotective and antioxidant agent in chronic cerebral hypoperfusion induced-neurodegeneration in rats. 2015-08-25 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/44589/1/44589.pdf Sayeed, Sayyada and Saxena, Anil Kumar and Talib, Norlelawati A. and Azzubaidi, Marwan Saad (2015) Assessment of reactive oxygen species status and neuroprotection by vitamin e in chronic cerebral ypoperfusion-induced neurodegeneration in rats. In: 29th Scientific Meeting of Malaysian Society of Pharmacology and Physiolgy (MSPP), 24-25 August 2015, Setia City Convention Centre, Shah Alam, Malaysia. http://medicine.uitm.edu.my/mspp2015/images/doc/MSPP2015_Brochure.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General) RM214 Diet therapy. Diet and dietectics in disease
spellingShingle	R Medicine (General) RM214 Diet therapy. Diet and dietectics in disease Sayeed, Sayyada Saxena, Anil Kumar Talib, Norlelawati A. Azzubaidi, Marwan Saad Assessment of reactive oxygen species status and neuroprotection by vitamin e in chronic cerebral ypoperfusion-induced neurodegeneration in rats
description	A persistent reduction in regional cerebral blood flow (CBF) compromises memory and cognitive functions in the elderly leading to neurological illnesses. To unravel the neuropathological consequences of a reduced CBF a similar condition has been created in rats by common carotid artery occlusion (2 vessel occlusion, 2VO). Since oxidative stress, leading to neuronal death, is a major risk factor in neurodegenerative disorders, the present study was designed to assess the neuroprotective role of Vitamin E (Vit E) in chronic cerebral hypoperfusion induced-neurodegeneration. After acclimatization, twenty four Sprague Dawley rats weighing 200-250 g were equally divided into three groups. Group A – sham control, Group B–2VO, and Group C–2VO-E (treated daily with Vit E, 100 mg/kg, orally following 2VO). On the 8th week, all the rats were euthanized and the hippocampi were isolated. Viable neuronal cell count in the hippocampal CA-1 region was estimated. The Isoprostane F2 (Iso-F2) levels were also measured in the brain homogenates to quantify the oxidative stress levels. There was significant difference in neuronal cell death in 2VO group as compared to sham group. In 2VO-E rats, the viable neuronal cell count of the hippocampal CA-1 region was significantly higher (p<0.05) as compared to the 2VO group. Moreover, Iso-F2 levels in 2VO group was significantly higher (p<0.05) as compared to 2VO-E group, implying high oxidative stress in 2VO group and reduction of oxidative stress levels in 2VO-E group. This study clearly demonstrates the effectiveness of Vit E as a neuroprotective and antioxidant agent in chronic cerebral hypoperfusion induced-neurodegeneration in rats.
format	Conference or Workshop Item
author	Sayeed, Sayyada Saxena, Anil Kumar Talib, Norlelawati A. Azzubaidi, Marwan Saad
author_facet	Sayeed, Sayyada Saxena, Anil Kumar Talib, Norlelawati A. Azzubaidi, Marwan Saad
author_sort	Sayeed, Sayyada
title	Assessment of reactive oxygen species status and neuroprotection by vitamin e in chronic cerebral ypoperfusion-induced neurodegeneration in rats
title_short	Assessment of reactive oxygen species status and neuroprotection by vitamin e in chronic cerebral ypoperfusion-induced neurodegeneration in rats
title_full	Assessment of reactive oxygen species status and neuroprotection by vitamin e in chronic cerebral ypoperfusion-induced neurodegeneration in rats
title_fullStr	Assessment of reactive oxygen species status and neuroprotection by vitamin e in chronic cerebral ypoperfusion-induced neurodegeneration in rats
title_full_unstemmed	Assessment of reactive oxygen species status and neuroprotection by vitamin e in chronic cerebral ypoperfusion-induced neurodegeneration in rats
title_sort	assessment of reactive oxygen species status and neuroprotection by vitamin e in chronic cerebral ypoperfusion-induced neurodegeneration in rats
publishDate	2015
url	http://irep.iium.edu.my/44589/ http://irep.iium.edu.my/44589/ http://irep.iium.edu.my/44589/1/44589.pdf
first_indexed	2023-09-18T21:03:22Z
last_indexed	2023-09-18T21:03:22Z
_version_	1777410772019707904

Assessment of reactive oxygen species status and neuroprotection by vitamin e in chronic cerebral ypoperfusion-induced neurodegeneration in rats

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