Identification and quantification of phenolic compounds in mangifera indica waterlily kernel and their free radical scavenging activity

The aim of this study was to determine phenolic compounds and antioxidant capacity of extract prepared from Mangifera indica waterlily kernel. Acidified methanolic extract prepared from M. indica waterlily kernel was analyzed. The total phenolic content was determined by the Folin_Ciocalteu met...

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Main Authors:	Al-Shwyeh, Hussah Abdullah, Sabo Muhammed, Abdulkarim, Abdullah, Rasedee, Mirghani, Mohamed Elwathig Saeed
Format:	Article
Language:	English
Published:	Journal of Advanced Agricultural Technologies 2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/44575/ http://irep.iium.edu.my/44575/ http://irep.iium.edu.my/44575/ http://irep.iium.edu.my/44575/1/J_of_Advanced_Agricultral_Technologies_%28Hussah%29.pdf

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spelling	iium-445752018-02-26T10:24:55Z http://irep.iium.edu.my/44575/ Identification and quantification of phenolic compounds in mangifera indica waterlily kernel and their free radical scavenging activity Al-Shwyeh, Hussah Abdullah Sabo Muhammed, Abdulkarim Abdullah, Rasedee Mirghani, Mohamed Elwathig Saeed Q Science (General) The aim of this study was to determine phenolic compounds and antioxidant capacity of extract prepared from Mangifera indica waterlily kernel. Acidified methanolic extract prepared from M. indica waterlily kernel was analyzed. The total phenolic content was determined by the Folin_Ciocalteu method. Antioxidant activity was measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and ferric-reducing antioxidant power (FRAP) assays. Characterization of phenolic compounds was conducted using HPLCDAD and TSQ-ESIMS and Peak identities were confirmed by comparing their retention times, UV_vis absorption spectra, and mass spectra with authentic standards. The total phenolic content of M. indica waterlily kernel was 8.6 mg GAE/g DW. The seeds extract showed a strong potency of antioxidant activity as confirmed by the DPPH radical scavenging activity, and FRAP assays. Epigallocatechin (EGC), chlorogenic acid, apiginin, and epicatechin were the major compounds among the 12 phenolics that have been identified and quantified in M. indica waterlily kernel with 21.8, 13.7, 3.2 and 2.7 mg/g M. indica waterlily kernel, respectively. The 12 phenolic compounds identified in M. indica waterlily kernel using HPLCDAD and TSQ-ESI-MS had high antioxidant activity and should be considered as a prospective antioxidant. Journal of Advanced Agricultural Technologies 2015-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/44575/1/J_of_Advanced_Agricultral_Technologies_%28Hussah%29.pdf Al-Shwyeh, Hussah Abdullah and Sabo Muhammed, Abdulkarim and Abdullah, Rasedee and Mirghani, Mohamed Elwathig Saeed (2015) Identification and quantification of phenolic compounds in mangifera indica waterlily kernel and their free radical scavenging activity. Journal of Advanced Agricultural Technologies, 2 (1). pp. 1-7. ISSN 2301-3737 (O); 2373-423X (P) http://www.joaat.com/index.php?m=content&c=index&a=show&catid=35&id=63 10.12720/joaat.2.1.1-7
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Al-Shwyeh, Hussah Abdullah Sabo Muhammed, Abdulkarim Abdullah, Rasedee Mirghani, Mohamed Elwathig Saeed Identification and quantification of phenolic compounds in mangifera indica waterlily kernel and their free radical scavenging activity
description	The aim of this study was to determine phenolic compounds and antioxidant capacity of extract prepared from Mangifera indica waterlily kernel. Acidified methanolic extract prepared from M. indica waterlily kernel was analyzed. The total phenolic content was determined by the Folin_Ciocalteu method. Antioxidant activity was measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and ferric-reducing antioxidant power (FRAP) assays. Characterization of phenolic compounds was conducted using HPLCDAD and TSQ-ESIMS and Peak identities were confirmed by comparing their retention times, UV_vis absorption spectra, and mass spectra with authentic standards. The total phenolic content of M. indica waterlily kernel was 8.6 mg GAE/g DW. The seeds extract showed a strong potency of antioxidant activity as confirmed by the DPPH radical scavenging activity, and FRAP assays. Epigallocatechin (EGC), chlorogenic acid, apiginin, and epicatechin were the major compounds among the 12 phenolics that have been identified and quantified in M. indica waterlily kernel with 21.8, 13.7, 3.2 and 2.7 mg/g M. indica waterlily kernel, respectively. The 12 phenolic compounds identified in M. indica waterlily kernel using HPLCDAD and TSQ-ESI-MS had high antioxidant activity and should be considered as a prospective antioxidant.
format	Article
author	Al-Shwyeh, Hussah Abdullah Sabo Muhammed, Abdulkarim Abdullah, Rasedee Mirghani, Mohamed Elwathig Saeed
author_facet	Al-Shwyeh, Hussah Abdullah Sabo Muhammed, Abdulkarim Abdullah, Rasedee Mirghani, Mohamed Elwathig Saeed
author_sort	Al-Shwyeh, Hussah Abdullah
title	Identification and quantification of phenolic compounds in mangifera indica waterlily kernel and their free radical scavenging activity
title_short	Identification and quantification of phenolic compounds in mangifera indica waterlily kernel and their free radical scavenging activity
title_full	Identification and quantification of phenolic compounds in mangifera indica waterlily kernel and their free radical scavenging activity
title_fullStr	Identification and quantification of phenolic compounds in mangifera indica waterlily kernel and their free radical scavenging activity
title_full_unstemmed	Identification and quantification of phenolic compounds in mangifera indica waterlily kernel and their free radical scavenging activity
title_sort	identification and quantification of phenolic compounds in mangifera indica waterlily kernel and their free radical scavenging activity
publisher	Journal of Advanced Agricultural Technologies
publishDate	2015
url	http://irep.iium.edu.my/44575/ http://irep.iium.edu.my/44575/ http://irep.iium.edu.my/44575/ http://irep.iium.edu.my/44575/1/J_of_Advanced_Agricultral_Technologies_%28Hussah%29.pdf
first_indexed	2023-09-18T21:03:22Z
last_indexed	2023-09-18T21:03:22Z
_version_	1777410771290947584

Identification and quantification of phenolic compounds in mangifera indica waterlily kernel and their free radical scavenging activity

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