Application of Plackett-Burman design for screening of parameters for the production of tetrathionate hydrolase by Thiobacillus ferrooxidans
Enzymatic devulcanisation is one of the methods of recycling waste rubber tire. Among all devulcanised methods that are introduced, biological method is a safer choice because it does not include any harmful or toxic chemicals, and is normally not energy intensive. The process deems more efficien...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Taylor's University
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/44467/ http://irep.iium.edu.my/44467/ http://irep.iium.edu.my/44467/1/P_67.pdf http://irep.iium.edu.my/44467/4/44467_Application%20of%20Plackett-Burman%20design%20for%20screening%20of%20parameters_SCOPUS.pdf |
| Summary: | Enzymatic devulcanisation is one of the methods of recycling waste rubber tire.
Among all devulcanised methods that are introduced, biological method is a
safer choice because it does not include any harmful or toxic chemicals, and is
normally not energy intensive. The process deems more efficient and less
expensive compared to various other devulcanisation methods. This method
was done by exposing a surface of a vulcanised rubber piece to at least one
enzyme, secreted from chemolithoautotrophic iron- and sulphur-oxidising
bacterium to break the sulphur bonds in the vulcanised rubber structure,
rendering it more acceptable to be recycled. By applying the enzymatic
devulcanisation, it is hoped that the amount of demand of raw rubber can be
reduced and at the same time helps to reduce the solid waste disposal problem.
In this study, the process parameters that affect the tetrathionate hydrolase
activity secreted from Thiobacillus ferrooxidans was screened using PlackettBurman
design (PBD). Among the various variables, results showed initial pH,
agitation rate, temperature of incubation, inoculum size and calcium chloride
concentration were determined as the most influential variables on tetrathionate
hydrolase production. |
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