Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice

The polyamine biosynthesis is a ubiquitous pathway with a significant role in cell growth , both normal and malignant. As polyamines are crucial in cellular growth and differentiation, they are linked to the development of cancer, with higher polyamine level observed in cancerous cells than in healt...

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Main Authors:	Abdul Ghani, Radiah, Md Yusof, Najwa Farhah, Nik Abdul Malek, Nik Nurasyikin, A.Talib, Norlelawati
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/44455/ http://irep.iium.edu.my/44455/1/44455.pdf

id	iium-44455
recordtype	eprints
spelling	iium-444552018-01-03T01:26:52Z http://irep.iium.edu.my/44455/ Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice Abdul Ghani, Radiah Md Yusof, Najwa Farhah Nik Abdul Malek, Nik Nurasyikin A.Talib, Norlelawati RB Pathology The polyamine biosynthesis is a ubiquitous pathway with a significant role in cell growth , both normal and malignant. As polyamines are crucial in cellular growth and differentiation, they are linked to the development of cancer, with higher polyamine level observed in cancerous cells than in healthy cells. Accordingly, suppressing the polyamine pathway has been found to disrupt tumour development. Chemoprevention is considered a more feasible option in cancer management than chemotherapy, with a focus on natural chemopreventive agent. Pomegranate is known to inhibit several progression of lung cancer, although prior studies on the chemopreventive effect of pomegranate on lung cancer did not explore into polyamine pathway. Hence, this study investigated the effect of pomegranate juice on the polyamine pathway, by focusing on the biosynthesis involving ornithine decarboxylase (ODC), the rate limiting enzyme in the pathway. Quantitative polymerase chain reaction (qPCR) was applied to quantify the changes in ODC gene expression in A549 cells treated with pomegranate. It was found that ODC expression was significantly inhibited compared to untreated cells, with a 48-fold difference. While this finding supports the hypothesis, there is much yet to be elucidated regarding its exact mechanism. 2015 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/44455/1/44455.pdf Abdul Ghani, Radiah and Md Yusof, Najwa Farhah and Nik Abdul Malek, Nik Nurasyikin and A.Talib, Norlelawati (2015) Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice. In: International Conference on Advancement in Science and Technology (iCAST 2015) - ‘Frontiers in Biotechnology’, 10-12 August 2015, Impiana Resort Cherating, Pahang, Malaysia. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RB Pathology
spellingShingle	RB Pathology Abdul Ghani, Radiah Md Yusof, Najwa Farhah Nik Abdul Malek, Nik Nurasyikin A.Talib, Norlelawati Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice
description	The polyamine biosynthesis is a ubiquitous pathway with a significant role in cell growth , both normal and malignant. As polyamines are crucial in cellular growth and differentiation, they are linked to the development of cancer, with higher polyamine level observed in cancerous cells than in healthy cells. Accordingly, suppressing the polyamine pathway has been found to disrupt tumour development. Chemoprevention is considered a more feasible option in cancer management than chemotherapy, with a focus on natural chemopreventive agent. Pomegranate is known to inhibit several progression of lung cancer, although prior studies on the chemopreventive effect of pomegranate on lung cancer did not explore into polyamine pathway. Hence, this study investigated the effect of pomegranate juice on the polyamine pathway, by focusing on the biosynthesis involving ornithine decarboxylase (ODC), the rate limiting enzyme in the pathway. Quantitative polymerase chain reaction (qPCR) was applied to quantify the changes in ODC gene expression in A549 cells treated with pomegranate. It was found that ODC expression was significantly inhibited compared to untreated cells, with a 48-fold difference. While this finding supports the hypothesis, there is much yet to be elucidated regarding its exact mechanism.
format	Conference or Workshop Item
author	Abdul Ghani, Radiah Md Yusof, Najwa Farhah Nik Abdul Malek, Nik Nurasyikin A.Talib, Norlelawati
author_facet	Abdul Ghani, Radiah Md Yusof, Najwa Farhah Nik Abdul Malek, Nik Nurasyikin A.Talib, Norlelawati
author_sort	Abdul Ghani, Radiah
title	Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice
title_short	Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice
title_full	Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice
title_fullStr	Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice
title_full_unstemmed	Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice
title_sort	ornithine decarboxylase gene expression in human lung carcinoma cells, a549 treated with pomegranate juice
publishDate	2015
url	http://irep.iium.edu.my/44455/ http://irep.iium.edu.my/44455/1/44455.pdf
first_indexed	2023-09-18T21:03:12Z
last_indexed	2023-09-18T21:03:12Z
_version_	1777410760945696768

Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice

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