Protein purification in chromatographic media using multiwall carbon nanotubes

Protein purification in chromatographic media using multiwall carbon nanotubes

Carbon Nanotubes (CNTs) had been synthesized by Double Stage Chemical Vapor Deposition (DS-CVD) at the gas phase with Hydrogen gas (H2) and acetylene (C2H2) as precursor gases. Moreover, the structure of CNTs, morphology and purity were characterized by using the Transmission Electron Microscope (TE...

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Main Authors: NM, Mubarak, Yusof, Faridah
Format: Article
Language:English
Published: OMICS International - Open Access Publisher 2015
Subjects:
TP155 Chemical engineering
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/44453/
http://irep.iium.edu.my/44453/
http://irep.iium.edu.my/44453/
http://irep.iium.edu.my/44453/1/P_60.pdf
id iium-44453
recordtype eprints
spelling iium-444532017-11-10T08:42:36Z http://irep.iium.edu.my/44453/ Protein purification in chromatographic media using multiwall carbon nanotubes NM, Mubarak Yusof, Faridah TP155 Chemical engineering TP248.13 Biotechnology Carbon Nanotubes (CNTs) had been synthesized by Double Stage Chemical Vapor Deposition (DS-CVD) at the gas phase with Hydrogen gas (H2) and acetylene (C2H2) as precursor gases. Moreover, the structure of CNTs, morphology and purity were characterized by using the Transmission Electron Microscope (TEM), Field Emission Scanning Electron Microscope (FESEM) and Thermogravimetric Analysis (TGA). Furthermore, the best conditions for the optimal CNT was reaction at temperature of 750°C, reaction time of 30 minutes, with gas flow rates H2 and C2H2 for 60 and 170 ml/min for respectively. The hydrochloric acid was used to purify produced CNT and then using the nitric acid and sulfuric treatment to functionalize. Moreover, functionalized CNTs, non-functionalized were used as column chromatographic media for skim latex protein purification. Thus, the results reveal that at pH7 and ionic strength 50 mM gives higher efficiency of protein removal from the skim latex. OMICS International - Open Access Publisher 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/44453/1/P_60.pdf NM, Mubarak and Yusof, Faridah (2015) Protein purification in chromatographic media using multiwall carbon nanotubes. Journal of Bioprocessing & Biotechniques, 5 (3). p. 1000214. ISSN 2155-9821 http://www.omicsonline.org/bioprocessing-biotechniques.php 10.4172/2155-9821.1000214
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP155 Chemical engineering
TP248.13 Biotechnology
spellingShingle TP155 Chemical engineering
TP248.13 Biotechnology
NM, Mubarak
Yusof, Faridah
Protein purification in chromatographic media using multiwall carbon nanotubes
description Carbon Nanotubes (CNTs) had been synthesized by Double Stage Chemical Vapor Deposition (DS-CVD) at the gas phase with Hydrogen gas (H2) and acetylene (C2H2) as precursor gases. Moreover, the structure of CNTs, morphology and purity were characterized by using the Transmission Electron Microscope (TEM), Field Emission Scanning Electron Microscope (FESEM) and Thermogravimetric Analysis (TGA). Furthermore, the best conditions for the optimal CNT was reaction at temperature of 750°C, reaction time of 30 minutes, with gas flow rates H2 and C2H2 for 60 and 170 ml/min for respectively. The hydrochloric acid was used to purify produced CNT and then using the nitric acid and sulfuric treatment to functionalize. Moreover, functionalized CNTs, non-functionalized were used as column chromatographic media for skim latex protein purification. Thus, the results reveal that at pH7 and ionic strength 50 mM gives higher efficiency of protein removal from the skim latex.
format Article
author NM, Mubarak
Yusof, Faridah
author_facet NM, Mubarak
Yusof, Faridah
author_sort NM, Mubarak
title Protein purification in chromatographic media using multiwall carbon nanotubes
title_short Protein purification in chromatographic media using multiwall carbon nanotubes
title_full Protein purification in chromatographic media using multiwall carbon nanotubes
title_fullStr Protein purification in chromatographic media using multiwall carbon nanotubes
title_full_unstemmed Protein purification in chromatographic media using multiwall carbon nanotubes
title_sort protein purification in chromatographic media using multiwall carbon nanotubes
publisher OMICS International - Open Access Publisher
publishDate 2015
url http://irep.iium.edu.my/44453/
http://irep.iium.edu.my/44453/
http://irep.iium.edu.my/44453/
http://irep.iium.edu.my/44453/1/P_60.pdf
first_indexed 2023-09-18T21:03:12Z
last_indexed 2023-09-18T21:03:12Z
_version_ 1777410760798896128

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