Comparison of cell disruption method for E. coli BL21-AI expressing recombinant bromelain
The often-encountered problem such as protein degradation has driven various methods of cell lysis post fermentation used. In this paper we compare the use of homogenization, sonication, sonication with lysozyme and chemical lysis using B-PER reagent with lysozyme to extract the recombinant bromelai...
| Main Authors: | , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/44445/ http://irep.iium.edu.my/44445/1/iCAST-Zatul.pdf |