Isolation of thermotolerant bacteria producing fibrinolytic enzyme

Fibrinolytic enzymes were widely used in the treatment of cardiovascular diseases. However, the efficiency of the commercial enzymes are still lack of perfection because there are many side effects as well as not tolerant to downstream processing such as heat sensitive during spray drying process. T...

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Main Authors:	Ab. Shukor , Nurul Aqilah, Amid, Azura, Abdul Karem, Mohd Ismail, Ab. Samad, Nadiah Syuhada
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/44442/ http://irep.iium.edu.my/44442/1/iCAST-Aqilah.pdf

id	iium-44442
recordtype	eprints
spelling	iium-444422016-01-01T06:47:13Z http://irep.iium.edu.my/44442/ Isolation of thermotolerant bacteria producing fibrinolytic enzyme Ab. Shukor , Nurul Aqilah Amid, Azura Abdul Karem, Mohd Ismail Ab. Samad, Nadiah Syuhada TP248.13 Biotechnology Fibrinolytic enzymes were widely used in the treatment of cardiovascular diseases. However, the efficiency of the commercial enzymes are still lack of perfection because there are many side effects as well as not tolerant to downstream processing such as heat sensitive during spray drying process. Therefore, this study presents newly isolated thermophiles bacteria producing fibrinolytic enzyme.. Sample was collected from Hot Spring Selayang at Selayang Selangor. Spread plate agar containing skim milk powder growth at pH 7, 53 for 24 hours was utilized to isolated thermo tolerant bacteria producing protease. Further isolation on bacteria producing fibrinolytic enzyme was carried out using fibrin plate. 16S rDNA gene sequence analysis was used to identify the genotype of the isolates. 27 colonies of thermotolerant bacteria were isolated, however, only 19 of them showing proteolytic activity. All of the 19 isolates are mortile and cocci in shapes, with 4 types of arrangement which are single, diplo (pair), strepto (chain) and staphylo (cluster). HSP04 and HSP11 are gram positive bacteria and others are gram negative. From 19 isolates only 6 were chosen for further analysis. HSP23 showed the highest fibrinolytic activity compared with others. HSP23 was identified as Bacillus lincheniformis with 98 % similarity to Bacillus lincheniformis DCM 13 and Bacillus lincheniformis strain ATCC 14580. Therefore, HSP23 is named as B. lincheniformis starin HSP23. 2015-08 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/44442/1/iCAST-Aqilah.pdf Ab. Shukor , Nurul Aqilah and Amid, Azura and Abdul Karem, Mohd Ismail and Ab. Samad, Nadiah Syuhada (2015) Isolation of thermotolerant bacteria producing fibrinolytic enzyme. In: International Conference on Advancement in Science and Technology (iCAST2010): The Science of Traditional Practices in Health and Disease, 26-29 Nov 2010, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Ab. Shukor , Nurul Aqilah Amid, Azura Abdul Karem, Mohd Ismail Ab. Samad, Nadiah Syuhada Isolation of thermotolerant bacteria producing fibrinolytic enzyme
description	Fibrinolytic enzymes were widely used in the treatment of cardiovascular diseases. However, the efficiency of the commercial enzymes are still lack of perfection because there are many side effects as well as not tolerant to downstream processing such as heat sensitive during spray drying process. Therefore, this study presents newly isolated thermophiles bacteria producing fibrinolytic enzyme.. Sample was collected from Hot Spring Selayang at Selayang Selangor. Spread plate agar containing skim milk powder growth at pH 7, 53 for 24 hours was utilized to isolated thermo tolerant bacteria producing protease. Further isolation on bacteria producing fibrinolytic enzyme was carried out using fibrin plate. 16S rDNA gene sequence analysis was used to identify the genotype of the isolates. 27 colonies of thermotolerant bacteria were isolated, however, only 19 of them showing proteolytic activity. All of the 19 isolates are mortile and cocci in shapes, with 4 types of arrangement which are single, diplo (pair), strepto (chain) and staphylo (cluster). HSP04 and HSP11 are gram positive bacteria and others are gram negative. From 19 isolates only 6 were chosen for further analysis. HSP23 showed the highest fibrinolytic activity compared with others. HSP23 was identified as Bacillus lincheniformis with 98 % similarity to Bacillus lincheniformis DCM 13 and Bacillus lincheniformis strain ATCC 14580. Therefore, HSP23 is named as B. lincheniformis starin HSP23.
format	Conference or Workshop Item
author	Ab. Shukor , Nurul Aqilah Amid, Azura Abdul Karem, Mohd Ismail Ab. Samad, Nadiah Syuhada
author_facet	Ab. Shukor , Nurul Aqilah Amid, Azura Abdul Karem, Mohd Ismail Ab. Samad, Nadiah Syuhada
author_sort	Ab. Shukor , Nurul Aqilah
title	Isolation of thermotolerant bacteria producing fibrinolytic enzyme
title_short	Isolation of thermotolerant bacteria producing fibrinolytic enzyme
title_full	Isolation of thermotolerant bacteria producing fibrinolytic enzyme
title_fullStr	Isolation of thermotolerant bacteria producing fibrinolytic enzyme
title_full_unstemmed	Isolation of thermotolerant bacteria producing fibrinolytic enzyme
title_sort	isolation of thermotolerant bacteria producing fibrinolytic enzyme
publishDate	2015
url	http://irep.iium.edu.my/44442/ http://irep.iium.edu.my/44442/1/iCAST-Aqilah.pdf
first_indexed	2023-09-18T21:03:10Z
last_indexed	2023-09-18T21:03:10Z
_version_	1777410759454621696

Isolation of thermotolerant bacteria producing fibrinolytic enzyme

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