Isolation, characterization and cytotoxic activity of arborinine from glycosmis pentaphylla (Retz.) DC. on human mammary gland adenocarcinoma (MCF7) cell line

Arborinine is an acridone alkaloid found exclusively in Rutacae family has showed cytotoxic potency against various cancer cell lines. The aims of this study were to isolate and identify arborinine from local G. pentapylla (Retz. D) and to determine its cytotoxicity activity on human mammary gland a...

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Main Authors:	Mohd Hussain, Nurlaili Najmi, Uzir, Bisha Fathamah, Mohd. Hassan, Norazian
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/44418/ http://irep.iium.edu.my/44418/1/44418.pdf

id	iium-44418
recordtype	eprints
spelling	iium-444182015-09-04T07:54:47Z http://irep.iium.edu.my/44418/ Isolation, characterization and cytotoxic activity of arborinine from glycosmis pentaphylla (Retz.) DC. on human mammary gland adenocarcinoma (MCF7) cell line Mohd Hussain, Nurlaili Najmi Uzir, Bisha Fathamah Mohd. Hassan, Norazian Q Science (General) Arborinine is an acridone alkaloid found exclusively in Rutacae family has showed cytotoxic potency against various cancer cell lines. The aims of this study were to isolate and identify arborinine from local G. pentapylla (Retz. D) and to determine its cytotoxicity activity on human mammary gland adenocarcinoma, MCF 7 cell line. The leaves of G. pentaphylla which were collected from Kelantan, Malaysia were air-dried and extracted with chloroform followed by acid-base extraction. Wet column chromatographic separation was employed to furnish arborinine. The structure of arborinine was elucidated by comparing its spectroscopic data to that of published reports. The cytotoxic effect of arborinine was investigated by means of the MTT assays, using human cell lines MCF7. The result showed that 0.1295 g (12.15%) of yellow needle-like crystals or arborinine was yielded from 4.4168 g of crude alkaloidal extract. Arborinine inhibit 50% of the MCF7 cells growth at 6.25±0.02uM after 72 hour of treatment and morphological changes were observed after 24, 48 and 72 hour incubation. The data presented suggest that arborinine can be regarded as an excellent starting structure for potential development of new anticancer agent. 2015-08-10 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/44418/1/44418.pdf Mohd Hussain, Nurlaili Najmi and Uzir, Bisha Fathamah and Mohd. Hassan, Norazian (2015) Isolation, characterization and cytotoxic activity of arborinine from glycosmis pentaphylla (Retz.) DC. on human mammary gland adenocarcinoma (MCF7) cell line. In: 5th International Conference on Advancement in Science and Technology (iCAST 2015), 10th-12th August 2015, Impiana Resort Cherating, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Mohd Hussain, Nurlaili Najmi Uzir, Bisha Fathamah Mohd. Hassan, Norazian Isolation, characterization and cytotoxic activity of arborinine from glycosmis pentaphylla (Retz.) DC. on human mammary gland adenocarcinoma (MCF7) cell line
description	Arborinine is an acridone alkaloid found exclusively in Rutacae family has showed cytotoxic potency against various cancer cell lines. The aims of this study were to isolate and identify arborinine from local G. pentapylla (Retz. D) and to determine its cytotoxicity activity on human mammary gland adenocarcinoma, MCF 7 cell line. The leaves of G. pentaphylla which were collected from Kelantan, Malaysia were air-dried and extracted with chloroform followed by acid-base extraction. Wet column chromatographic separation was employed to furnish arborinine. The structure of arborinine was elucidated by comparing its spectroscopic data to that of published reports. The cytotoxic effect of arborinine was investigated by means of the MTT assays, using human cell lines MCF7. The result showed that 0.1295 g (12.15%) of yellow needle-like crystals or arborinine was yielded from 4.4168 g of crude alkaloidal extract. Arborinine inhibit 50% of the MCF7 cells growth at 6.25±0.02uM after 72 hour of treatment and morphological changes were observed after 24, 48 and 72 hour incubation. The data presented suggest that arborinine can be regarded as an excellent starting structure for potential development of new anticancer agent.
format	Conference or Workshop Item
author	Mohd Hussain, Nurlaili Najmi Uzir, Bisha Fathamah Mohd. Hassan, Norazian
author_facet	Mohd Hussain, Nurlaili Najmi Uzir, Bisha Fathamah Mohd. Hassan, Norazian
author_sort	Mohd Hussain, Nurlaili Najmi
title	Isolation, characterization and cytotoxic activity of arborinine from glycosmis pentaphylla (Retz.) DC. on human mammary gland adenocarcinoma (MCF7) cell line
title_short	Isolation, characterization and cytotoxic activity of arborinine from glycosmis pentaphylla (Retz.) DC. on human mammary gland adenocarcinoma (MCF7) cell line
title_full	Isolation, characterization and cytotoxic activity of arborinine from glycosmis pentaphylla (Retz.) DC. on human mammary gland adenocarcinoma (MCF7) cell line
title_fullStr	Isolation, characterization and cytotoxic activity of arborinine from glycosmis pentaphylla (Retz.) DC. on human mammary gland adenocarcinoma (MCF7) cell line
title_full_unstemmed	Isolation, characterization and cytotoxic activity of arborinine from glycosmis pentaphylla (Retz.) DC. on human mammary gland adenocarcinoma (MCF7) cell line
title_sort	isolation, characterization and cytotoxic activity of arborinine from glycosmis pentaphylla (retz.) dc. on human mammary gland adenocarcinoma (mcf7) cell line
publishDate	2015
url	http://irep.iium.edu.my/44418/ http://irep.iium.edu.my/44418/1/44418.pdf
first_indexed	2023-09-18T21:03:09Z
last_indexed	2023-09-18T21:03:09Z
_version_	1777410757787385856

Isolation, characterization and cytotoxic activity of arborinine from glycosmis pentaphylla (Retz.) DC. on human mammary gland adenocarcinoma (MCF7) cell line

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