Antioxidant and antimicrobial properties of Tinospora crispa stems methanolic extract

Apart from being the primary source of food to other living things, plants also have medicinal value to treat various kinds of diseases. In recent years, it has been proposed that the extract from plants may be used as natural antioxidants which can help to prevent the generation of carcinogens in h...

Full description

Bibliographic Details
Main Authors:	Sulaiman, Fatimatul Akmal, Fuad, Nurfarahain, Rahman, Farawahida, Iqbal, Anwar, Darnis, Deny Susanti
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/44397/ http://irep.iium.edu.my/44397/1/44397.pdf

id	iium-44397
recordtype	eprints
spelling	iium-443972018-05-22T03:50:26Z http://irep.iium.edu.my/44397/ Antioxidant and antimicrobial properties of Tinospora crispa stems methanolic extract Sulaiman, Fatimatul Akmal Fuad, Nurfarahain Rahman, Farawahida Iqbal, Anwar Darnis, Deny Susanti Q Science (General) Apart from being the primary source of food to other living things, plants also have medicinal value to treat various kinds of diseases. In recent years, it has been proposed that the extract from plants may be used as natural antioxidants which can help to prevent the generation of carcinogens in human body. In addition, plants also have antimicrobial agents to inhibit the growth of pathogenic microbes. This study was intended to investigate the antioxidant properties and antimicrobial activity of methanolic extract of Tinospora crispa stems extracted using soxhlet extraction method. The antimicrobial properties of T. crispa stems were tested using disc diffusion method against Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans IMR C S23/11 A and Saccharomyces cerevisiae IMR S 617/068. The antioxidant properties of the methanolic extract were investigated by using TPC, TFC, DPPH Free Radical Scavenging Assay and β –carotene bleaching assay. The TPC value was 6.12 g GAE/100 g of dried extract while the TFC value was 55.58 g QE/100 g of dried extract. The IC50 of DPPH scavenging assay for the methanolic extract and ascorbic acid were 0.21 and 0.04 mg/mL respectively. The average percentage of β–carotene bleaching assay was 38.3 % as compared to BHT, which was 45.1%. The disc diffusion method showed no inhibition zone against all the strains of microorganisms at all concentrations of the extracts (0.5, 1.0, 2.5 and 5.0 mg/disc). 2015 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/44397/1/44397.pdf Sulaiman, Fatimatul Akmal and Fuad, Nurfarahain and Rahman, Farawahida and Iqbal, Anwar and Darnis, Deny Susanti (2015) Antioxidant and antimicrobial properties of Tinospora crispa stems methanolic extract. In: International Conference on Advancement in Science and Technology (iCAST 2015) - ‘Frontiers in Biotechnology’, 10th-12th August 2015, Cherating, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Sulaiman, Fatimatul Akmal Fuad, Nurfarahain Rahman, Farawahida Iqbal, Anwar Darnis, Deny Susanti Antioxidant and antimicrobial properties of Tinospora crispa stems methanolic extract
description	Apart from being the primary source of food to other living things, plants also have medicinal value to treat various kinds of diseases. In recent years, it has been proposed that the extract from plants may be used as natural antioxidants which can help to prevent the generation of carcinogens in human body. In addition, plants also have antimicrobial agents to inhibit the growth of pathogenic microbes. This study was intended to investigate the antioxidant properties and antimicrobial activity of methanolic extract of Tinospora crispa stems extracted using soxhlet extraction method. The antimicrobial properties of T. crispa stems were tested using disc diffusion method against Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans IMR C S23/11 A and Saccharomyces cerevisiae IMR S 617/068. The antioxidant properties of the methanolic extract were investigated by using TPC, TFC, DPPH Free Radical Scavenging Assay and β –carotene bleaching assay. The TPC value was 6.12 g GAE/100 g of dried extract while the TFC value was 55.58 g QE/100 g of dried extract. The IC50 of DPPH scavenging assay for the methanolic extract and ascorbic acid were 0.21 and 0.04 mg/mL respectively. The average percentage of β–carotene bleaching assay was 38.3 % as compared to BHT, which was 45.1%. The disc diffusion method showed no inhibition zone against all the strains of microorganisms at all concentrations of the extracts (0.5, 1.0, 2.5 and 5.0 mg/disc).
format	Conference or Workshop Item
author	Sulaiman, Fatimatul Akmal Fuad, Nurfarahain Rahman, Farawahida Iqbal, Anwar Darnis, Deny Susanti
author_facet	Sulaiman, Fatimatul Akmal Fuad, Nurfarahain Rahman, Farawahida Iqbal, Anwar Darnis, Deny Susanti
author_sort	Sulaiman, Fatimatul Akmal
title	Antioxidant and antimicrobial properties of Tinospora crispa stems methanolic extract
title_short	Antioxidant and antimicrobial properties of Tinospora crispa stems methanolic extract
title_full	Antioxidant and antimicrobial properties of Tinospora crispa stems methanolic extract
title_fullStr	Antioxidant and antimicrobial properties of Tinospora crispa stems methanolic extract
title_full_unstemmed	Antioxidant and antimicrobial properties of Tinospora crispa stems methanolic extract
title_sort	antioxidant and antimicrobial properties of tinospora crispa stems methanolic extract
publishDate	2015
url	http://irep.iium.edu.my/44397/ http://irep.iium.edu.my/44397/1/44397.pdf
first_indexed	2023-09-18T21:03:07Z
last_indexed	2023-09-18T21:03:07Z
_version_	1777410755867443200

Antioxidant and antimicrobial properties of Tinospora crispa stems methanolic extract

Similar Items