Cytotoxic and antimicrobial activities of alkaloids from tabernaemontana corymbosa

The investigations were made based on the fact that alkaloids from Apocynaceae family are potential for medicinal purposes. The aim of the study was to explore the potential cytotoxic and antimicrobial activities of alkaloids extracted from T. corymbosa and to provide scientific basis for further st...

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Main Authors:	Bakhtiar, M. Taher, Nur ‘Izzati, Mansor, Darnis, Deny Susanti
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/4439/ http://irep.iium.edu.my/4439/ http://irep.iium.edu.my/4439/1/Abstract_Taher_AASP2011.pdf

id	iium-4439
recordtype	eprints
spelling	iium-44392014-12-09T01:30:13Z http://irep.iium.edu.my/4439/ Cytotoxic and antimicrobial activities of alkaloids from tabernaemontana corymbosa Bakhtiar, M. Taher Nur ‘Izzati, Mansor Darnis, Deny Susanti RS Pharmacy and materia medica The investigations were made based on the fact that alkaloids from Apocynaceae family are potential for medicinal purposes. The aim of the study was to explore the potential cytotoxic and antimicrobial activities of alkaloids extracted from T. corymbosa and to provide scientific basis for further studies of T. corymbosa. The alkaloids were extracted from stembarks of Tabernaemontana corymbosa using maceration and acid-base extraction methods. Then, the extracts were tested for their cytotoxic and antimicrobial activities. The cytotoxic activity was studied in vitro using human non-small lung carcinoma (A549) and human cervical carcinoma (C33A). The cells were exposed to three crude alkaloid extracts of T. corymbosa. The cytotoxic effects of these crude alkaloid extracts were evaluated by MTT assay. In antimicrobial activity study, the crude alkaloid extracts of T. corymbosa were tested against four bacterial and two fungi species by using disc diffusion method. Bacillus cereus ATCC11778, Pseudomonas aeruginosa ATCC27853, Staphylococcus aureus ATCC25923, Escherichia coli ATCC35218, Cryptococcus neoformans ATCC90112 and Candida albicans ATCC10231 were used in the study. Cytotoxicity study showed that only crude B alkaloids exhibited cytotoxic effect against A549 and C33A cancer cells, giving an IC50 as low as 7.81 μg/mL and 3.91 μg/mL, respectively. In antimicrobial activity, the zones of inhibition against the tested bacteria were found in the range of 7.00 to 14.75 mm, along with their MIC and MBC/MFC values ranging from 0.37-1.11 mg/mL and 3.33-10.00 mg/mL, respectively. Those results suggest that T. corymbosa contains alkaloids that active as cytotoxic and antimicrobial agents. 2011-06 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/4439/1/Abstract_Taher_AASP2011.pdf Bakhtiar, M. Taher and Nur ‘Izzati, Mansor and Darnis, Deny Susanti (2011) Cytotoxic and antimicrobial activities of alkaloids from tabernaemontana corymbosa. In: 5th AASP Conference, 17-19 June 2011, Bandung. http://www.aasp2011.fa.itb.ac.id/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Bakhtiar, M. Taher Nur ‘Izzati, Mansor Darnis, Deny Susanti Cytotoxic and antimicrobial activities of alkaloids from tabernaemontana corymbosa
description	The investigations were made based on the fact that alkaloids from Apocynaceae family are potential for medicinal purposes. The aim of the study was to explore the potential cytotoxic and antimicrobial activities of alkaloids extracted from T. corymbosa and to provide scientific basis for further studies of T. corymbosa. The alkaloids were extracted from stembarks of Tabernaemontana corymbosa using maceration and acid-base extraction methods. Then, the extracts were tested for their cytotoxic and antimicrobial activities. The cytotoxic activity was studied in vitro using human non-small lung carcinoma (A549) and human cervical carcinoma (C33A). The cells were exposed to three crude alkaloid extracts of T. corymbosa. The cytotoxic effects of these crude alkaloid extracts were evaluated by MTT assay. In antimicrobial activity study, the crude alkaloid extracts of T. corymbosa were tested against four bacterial and two fungi species by using disc diffusion method. Bacillus cereus ATCC11778, Pseudomonas aeruginosa ATCC27853, Staphylococcus aureus ATCC25923, Escherichia coli ATCC35218, Cryptococcus neoformans ATCC90112 and Candida albicans ATCC10231 were used in the study. Cytotoxicity study showed that only crude B alkaloids exhibited cytotoxic effect against A549 and C33A cancer cells, giving an IC50 as low as 7.81 μg/mL and 3.91 μg/mL, respectively. In antimicrobial activity, the zones of inhibition against the tested bacteria were found in the range of 7.00 to 14.75 mm, along with their MIC and MBC/MFC values ranging from 0.37-1.11 mg/mL and 3.33-10.00 mg/mL, respectively. Those results suggest that T. corymbosa contains alkaloids that active as cytotoxic and antimicrobial agents.
format	Conference or Workshop Item
author	Bakhtiar, M. Taher Nur ‘Izzati, Mansor Darnis, Deny Susanti
author_facet	Bakhtiar, M. Taher Nur ‘Izzati, Mansor Darnis, Deny Susanti
author_sort	Bakhtiar, M. Taher
title	Cytotoxic and antimicrobial activities of alkaloids from tabernaemontana corymbosa
title_short	Cytotoxic and antimicrobial activities of alkaloids from tabernaemontana corymbosa
title_full	Cytotoxic and antimicrobial activities of alkaloids from tabernaemontana corymbosa
title_fullStr	Cytotoxic and antimicrobial activities of alkaloids from tabernaemontana corymbosa
title_full_unstemmed	Cytotoxic and antimicrobial activities of alkaloids from tabernaemontana corymbosa
title_sort	cytotoxic and antimicrobial activities of alkaloids from tabernaemontana corymbosa
publishDate	2011
url	http://irep.iium.edu.my/4439/ http://irep.iium.edu.my/4439/ http://irep.iium.edu.my/4439/1/Abstract_Taher_AASP2011.pdf
first_indexed	2023-09-18T20:12:39Z
last_indexed	2023-09-18T20:12:39Z
_version_	1777407581006856192

Cytotoxic and antimicrobial activities of alkaloids from tabernaemontana corymbosa

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