An adjustable force soft-contact lithography for patterning biomolecules on 3D topologies
In this paper, we report on a precision patterning technique using an adjustable-force soft-landing contact lithography that can be used to stamp functional biomolecules on planar and non-planar fragile surfaces. We have successfully patterned fluorescent antibodies (Alexa 568 antigoat IgG) on...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
IEEE
2005
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/44331/ http://irep.iium.edu.my/44331/ http://irep.iium.edu.my/44331/ http://irep.iium.edu.my/44331/7/44331%20ADJUSTABLE-FORCE%20SOFT-LANDING%20CONTACT%20LITHOGRAPHY.pdf |
| Summary: | In this paper, we report on a precision patterning
technique using an adjustable-force soft-landing
contact lithography that can be used to stamp
functional biomolecules on planar and non-planar
fragile surfaces. We have successfully patterned
fluorescent antibodies (Alexa 568 antigoat IgG) on
high frequency silicon microresonators with
central vibrating bodies ranging from 20x10 to
480x240pm2 and arrays of 40pm diameter circular
patterns with superior uniformity and without loss
in proteins functionality. The printed antibodies
were tested for effectiveness in binding fluorescent
conjugated antigen (488 labeled goat anti-mouse
IgG), and were shown to retain nearly-optimal
bindings. |
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