Preparation of natural chitosan from pens of squid Loligo spp.

Preparation of natural chitosan from pens of squid Loligo spp.

Natural chitosan derived from chitin can be obtained from crustacean shells as well as squid pen which usually considered as waste materials. Natural chitosan is biodegradable, biocompatible, non-toxic, anti-microbial, and possesses adsorption properties. Squid chitosan which is derived from β-chiti...

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Main Authors: Ahmad, Hawa Mas Azmar, Ab. Hadi, Hazrina, Md. Jaffri, Juliana
Format: Conference or Workshop Item
Language:English
Published: 2015
Subjects:
RS Pharmacy and materia medica
Online Access:http://irep.iium.edu.my/44314/
http://irep.iium.edu.my/44314/1/44314.pdf
id iium-44314
recordtype eprints
spelling iium-443142015-09-10T03:54:44Z http://irep.iium.edu.my/44314/ Preparation of natural chitosan from pens of squid Loligo spp. Ahmad, Hawa Mas Azmar Ab. Hadi, Hazrina Md. Jaffri, Juliana RS Pharmacy and materia medica Natural chitosan derived from chitin can be obtained from crustacean shells as well as squid pen which usually considered as waste materials. Natural chitosan is biodegradable, biocompatible, non-toxic, anti-microbial, and possesses adsorption properties. Squid chitosan which is derived from β-chitin can be used as thickening agent in cosmetics formulation with advantage of scar removal property. The squid pens collected were washed and air dried before grounded into powder. 20 g of squid pen powder was treated in 300 mL of 1N hydrochloric acid for 2 hours in stirring condition for demineralisation. The mixture was filtered and washed with phosphate buffer pH 7 and dried in oven (60°C) overnight. Later, the demineralized powder was treated with 1M sodium hydroxide (NaOH) for 2 hours, filtered and washed with phosphate buffer pH 8 and dried in the dried in oven (60°C) overnight for deproteination. The deproteinated sample was further undergo deacetylation of chitin with 50% NaOH under reflux at 95°C for 3 hours using soxhlet apparatus. The deacetylated chitin was later washed with water, filtered, and dried in oven overnight. The dried chitin was purified into chitosan by dissolving in 1% acetic acid for 2 hours in stirring condition and filtered. The filtrate solution was later kept in -80°C freezer and dried in freeze dryer for 2 days. White powder or chitosan formed was weighed and its percentage yield was calculated. The chitosan was characterized using attenuated Fourier-Transform infrared spectroscopy (ATR-FTIR). Percentage yield obtained from the preparation of natural chitosan from squid pens was 3.5% (0.7 g). ATR-FTIR analysis showed that the natural chitosan has similar spectrum to the commercialized chitosan. The method used was convenient in terms of time-consumed and its productivity. A significant percentage yield of natural chitosan obtained from squid pen may turn waste materials into important and beneficial components. 2015 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/44314/1/44314.pdf Ahmad, Hawa Mas Azmar and Ab. Hadi, Hazrina and Md. Jaffri, Juliana (2015) Preparation of natural chitosan from pens of squid Loligo spp. In: 5th International Conference on Advancement in Science and Technology (iCAST 2015), 10th-12th August 2015, Impiana Resort Cherating, Kuantan, Pahang. (Unpublished)
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Ahmad, Hawa Mas Azmar
Ab. Hadi, Hazrina
Md. Jaffri, Juliana
Preparation of natural chitosan from pens of squid Loligo spp.
description Natural chitosan derived from chitin can be obtained from crustacean shells as well as squid pen which usually considered as waste materials. Natural chitosan is biodegradable, biocompatible, non-toxic, anti-microbial, and possesses adsorption properties. Squid chitosan which is derived from β-chitin can be used as thickening agent in cosmetics formulation with advantage of scar removal property. The squid pens collected were washed and air dried before grounded into powder. 20 g of squid pen powder was treated in 300 mL of 1N hydrochloric acid for 2 hours in stirring condition for demineralisation. The mixture was filtered and washed with phosphate buffer pH 7 and dried in oven (60°C) overnight. Later, the demineralized powder was treated with 1M sodium hydroxide (NaOH) for 2 hours, filtered and washed with phosphate buffer pH 8 and dried in the dried in oven (60°C) overnight for deproteination. The deproteinated sample was further undergo deacetylation of chitin with 50% NaOH under reflux at 95°C for 3 hours using soxhlet apparatus. The deacetylated chitin was later washed with water, filtered, and dried in oven overnight. The dried chitin was purified into chitosan by dissolving in 1% acetic acid for 2 hours in stirring condition and filtered. The filtrate solution was later kept in -80°C freezer and dried in freeze dryer for 2 days. White powder or chitosan formed was weighed and its percentage yield was calculated. The chitosan was characterized using attenuated Fourier-Transform infrared spectroscopy (ATR-FTIR). Percentage yield obtained from the preparation of natural chitosan from squid pens was 3.5% (0.7 g). ATR-FTIR analysis showed that the natural chitosan has similar spectrum to the commercialized chitosan. The method used was convenient in terms of time-consumed and its productivity. A significant percentage yield of natural chitosan obtained from squid pen may turn waste materials into important and beneficial components.
format Conference or Workshop Item
author Ahmad, Hawa Mas Azmar
Ab. Hadi, Hazrina
Md. Jaffri, Juliana
author_facet Ahmad, Hawa Mas Azmar
Ab. Hadi, Hazrina
Md. Jaffri, Juliana
author_sort Ahmad, Hawa Mas Azmar
title Preparation of natural chitosan from pens of squid Loligo spp.
title_short Preparation of natural chitosan from pens of squid Loligo spp.
title_full Preparation of natural chitosan from pens of squid Loligo spp.
title_fullStr Preparation of natural chitosan from pens of squid Loligo spp.
title_full_unstemmed Preparation of natural chitosan from pens of squid Loligo spp.
title_sort preparation of natural chitosan from pens of squid loligo spp.
publishDate 2015
url http://irep.iium.edu.my/44314/
http://irep.iium.edu.my/44314/1/44314.pdf
first_indexed 2023-09-18T21:03:01Z
last_indexed 2023-09-18T21:03:01Z
_version_ 1777410749637853184

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