Preparation of natural chitosan from pens of squid Loligo spp.
Natural chitosan derived from chitin can be obtained from crustacean shells as well as squid pen which usually considered as waste materials. Natural chitosan is biodegradable, biocompatible, non-toxic, anti-microbial, and possesses adsorption properties. Squid chitosan which is derived from β-chiti...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/44314/ http://irep.iium.edu.my/44314/1/44314.pdf |
| Summary: | Natural chitosan derived from chitin can be obtained from crustacean shells as well as squid pen which usually considered as waste materials. Natural chitosan is biodegradable, biocompatible, non-toxic, anti-microbial, and possesses adsorption properties. Squid chitosan which is derived from β-chitin can be used as thickening agent in cosmetics formulation with advantage of scar removal property. The squid pens collected were washed and air dried before grounded into powder. 20 g of squid pen powder was treated in 300 mL of 1N hydrochloric acid for 2 hours in stirring condition for demineralisation. The mixture was filtered and washed with phosphate buffer pH 7 and dried in oven (60°C) overnight. Later, the demineralized powder was treated with 1M sodium hydroxide (NaOH) for 2 hours, filtered and washed with phosphate buffer pH 8 and dried in the dried in oven (60°C) overnight for deproteination. The deproteinated sample was further undergo deacetylation of chitin with 50% NaOH under reflux at 95°C for 3 hours using soxhlet apparatus. The deacetylated chitin was later washed with water, filtered, and dried in oven overnight. The dried chitin was purified into chitosan by dissolving in 1% acetic acid for 2 hours in stirring condition and filtered. The filtrate solution was later kept in -80°C freezer and dried in freeze dryer for 2 days. White powder or chitosan formed was weighed and its percentage yield was calculated. The chitosan was characterized using attenuated Fourier-Transform infrared spectroscopy (ATR-FTIR). Percentage yield obtained from the preparation of natural chitosan from squid pens was 3.5% (0.7 g). ATR-FTIR analysis showed that the natural chitosan has similar spectrum to the commercialized chitosan. The method used was convenient in terms of time-consumed and its productivity. A significant percentage yield of natural chitosan obtained from squid pen may turn waste materials into important and beneficial components. |
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