Improvement of halophilic cellulase production from locally isolated fungal strain

Halophilic cellulases from the newly isolated fungus, Aspergillus terreus UniMAP AA-6 were found to be useful for in situ saccharification of ionic liquids treated lignocelluloses. Efforts have been taken to improve the enzyme production through statistical optimization approach namely Plackett–B...

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Main Authors:	Nagoor Gunny, Ahmad Anas, Arbain, Dachyar, Jamal, Parveen, Gumba, Rizo Edwin
Format:	Article
Language:	English
Published:	Elsevier Ltd. 2015
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/44276/ http://irep.iium.edu.my/44276/ http://irep.iium.edu.my/44276/ http://irep.iium.edu.my/44276/1/Saudi_paper.pdf

id	iium-44276
recordtype	eprints
spelling	iium-442762015-10-11T13:15:11Z http://irep.iium.edu.my/44276/ Improvement of halophilic cellulase production from locally isolated fungal strain Nagoor Gunny, Ahmad Anas Arbain, Dachyar Jamal, Parveen Gumba, Rizo Edwin T Technology (General) Halophilic cellulases from the newly isolated fungus, Aspergillus terreus UniMAP AA-6 were found to be useful for in situ saccharification of ionic liquids treated lignocelluloses. Efforts have been taken to improve the enzyme production through statistical optimization approach namely Plackett–Burman design and the Face Centered Central Composite Design (FCCCD). Plackett–Burman experimental design was used to screen the medium components and process conditions. It was found that carboxymethylcellulose (CMC), FeSO4Æ7H2O, NaCl, MgSO4Æ7H2O, peptone, agitation speed and inoculum size significantly influence the production of halophilic cellulase. On the other hand, KH2PO4, KOH, yeast extract and temperature had a negative effect on enzyme production. Further optimization through FCCCD revealed that the optimization approach improved halophilic cellulase production from 0.029 U/ml to 0.0625 U/ml, which was approximately 2.2-times greater than before optimization. Elsevier Ltd. 2015-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/44276/1/Saudi_paper.pdf Nagoor Gunny, Ahmad Anas and Arbain, Dachyar and Jamal, Parveen and Gumba, Rizo Edwin (2015) Improvement of halophilic cellulase production from locally isolated fungal strain. Saudi Journal of Biological Sciences, 22 (4). pp. 476-483. http://www.sciencedirect.com/science/article/pii/S1319562X14001673# 10.1016/j.sjbs.2014.11.021
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) Nagoor Gunny, Ahmad Anas Arbain, Dachyar Jamal, Parveen Gumba, Rizo Edwin Improvement of halophilic cellulase production from locally isolated fungal strain
description	Halophilic cellulases from the newly isolated fungus, Aspergillus terreus UniMAP AA-6 were found to be useful for in situ saccharification of ionic liquids treated lignocelluloses. Efforts have been taken to improve the enzyme production through statistical optimization approach namely Plackett–Burman design and the Face Centered Central Composite Design (FCCCD). Plackett–Burman experimental design was used to screen the medium components and process conditions. It was found that carboxymethylcellulose (CMC), FeSO4Æ7H2O, NaCl, MgSO4Æ7H2O, peptone, agitation speed and inoculum size significantly influence the production of halophilic cellulase. On the other hand, KH2PO4, KOH, yeast extract and temperature had a negative effect on enzyme production. Further optimization through FCCCD revealed that the optimization approach improved halophilic cellulase production from 0.029 U/ml to 0.0625 U/ml, which was approximately 2.2-times greater than before optimization.
format	Article
author	Nagoor Gunny, Ahmad Anas Arbain, Dachyar Jamal, Parveen Gumba, Rizo Edwin
author_facet	Nagoor Gunny, Ahmad Anas Arbain, Dachyar Jamal, Parveen Gumba, Rizo Edwin
author_sort	Nagoor Gunny, Ahmad Anas
title	Improvement of halophilic cellulase production from locally isolated fungal strain
title_short	Improvement of halophilic cellulase production from locally isolated fungal strain
title_full	Improvement of halophilic cellulase production from locally isolated fungal strain
title_fullStr	Improvement of halophilic cellulase production from locally isolated fungal strain
title_full_unstemmed	Improvement of halophilic cellulase production from locally isolated fungal strain
title_sort	improvement of halophilic cellulase production from locally isolated fungal strain
publisher	Elsevier Ltd.
publishDate	2015
url	http://irep.iium.edu.my/44276/ http://irep.iium.edu.my/44276/ http://irep.iium.edu.my/44276/ http://irep.iium.edu.my/44276/1/Saudi_paper.pdf
first_indexed	2023-09-18T21:02:58Z
last_indexed	2023-09-18T21:02:58Z
_version_	1777410746114637824

Improvement of halophilic cellulase production from locally isolated fungal strain

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