A novel animal model for subcutaneous soft tissue infection
In order to simultaneously monitor neutrophil migration in vivo during inflammation, we developed a novel animal model for subcutaneous soft tissue infection using transgenic mouse bearing fluorescence-positive neutrophils and bioluminescent methicillin-resistant Staphylococcus aureus (MRSA). Metho...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
J-STAGE
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/44152/ http://irep.iium.edu.my/44152/ http://irep.iium.edu.my/44152/ http://irep.iium.edu.my/44152/1/A_Novel_Animal_Model_for_Subcutaneous_Soft_Tissue_Infection.pdf |
| Summary: | In order to simultaneously monitor neutrophil migration in vivo during inflammation, we developed a novel animal model for subcutaneous soft tissue infection using transgenic mouse bearing fluorescence-positive neutrophils and bioluminescent methicillin-resistant Staphylococcus aureus (MRSA).
Methods: Eight to nine-week-old male lys-EGFP C57BL/6 were anesthetized (50 mg/kg pentobarbital sodium, ip) and 1.0 ×107 CFU of bioluminescent MRSA (Xen31, PerkinElmer) were subcutaneously injected. Control group was injected with PBS while the treatment group was given Arbekacin (25 mg/kg) intravenously via tail vein. For the evaluation of MRSA activity and neutrophil accumulation, an in vivo imaging system (LAS-4000, GE) was performed.
Results: Both groups displayed similar pattern with significant drop in MRSA bioluminescence and neutrophil fluorescence peaked on day 1. However, bacterial infection completely resolved in treatment group by day 6 with gradual decline in neutrophil fluorescence.
Conclusions: This animal model could be a competent method for assessment of subcutaneous soft tissue infections.
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