Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications

Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have studied a novel application of microwave radiation to prepare decellularized tissue for tissue-engineered scaffolds. The aim of the present study is to investigate the possibility of microwave effe...

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Main Authors:	Noor Azmi, Azran Azhim, Narita, Y., Muramatsu, K., Morimoto, Y., Tanaka, Masato
Format:	Conference or Workshop Item
Language:	English English
Published:	Springer Berlin Heidelberg 2010
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/44078/ http://irep.iium.edu.my/44078/ http://irep.iium.edu.my/44078/ http://irep.iium.edu.my/44078/1/Decellularization_of_Living_Tissue_Using_Microwave_Chemical_Process_for_Tissue-Engineered_Scaffold_Applications.pdf http://irep.iium.edu.my/44078/4/44078.pdf

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recordtype	eprints
spelling	iium-440782015-08-04T07:48:27Z http://irep.iium.edu.my/44078/ Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications Noor Azmi, Azran Azhim Narita, Y. Muramatsu, K. Morimoto, Y. Tanaka, Masato TP248.13 Biotechnology Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have studied a novel application of microwave radiation to prepare decellularized tissue for tissue-engineered scaffolds. The aim of the present study is to investigate the possibility of microwave effects on decellularization of soft and cartilage tissues. The targeted tissues were decellularized using 2450 MHz of microwave radiation in pulse-circulated 0.9% sodium chloride constituent of 1% sodium deoxycholate solution. To evaluate tissue integrity and cell removal, we used hematoxylin-eosin staining and scanning electron microscope. To investigate the effect of microwave power on targeted sample, we evaluate the radiated microwave distribution in microwave oven using thermograpic camera. Histological results suggest that microwave power influenced the decellularization of samples. It is significantly decellularized, when compared with the other preparation methods. Due to distributions of microwave radiation in the oven are non-uniform, the efficiency of cell removal can be improved by fitting the sample at uniform distribution and higher radiation spot of microwave. In conclusion, combination of microwave radiation and chemically detergent process has potential to decellularize various tissues, even tendon tissue. However, optimization of electromagnetic field in the oven is needed to perform in the further study to improve the decellularization efficiency. Springer Berlin Heidelberg 2010 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/44078/1/Decellularization_of_Living_Tissue_Using_Microwave_Chemical_Process_for_Tissue-Engineered_Scaffold_Applications.pdf application/pdf en http://irep.iium.edu.my/44078/4/44078.pdf Noor Azmi, Azran Azhim and Narita, Y. and Muramatsu, K. and Morimoto, Y. and Tanaka, Masato (2010) Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications. In: 6th World Congress of Biomechanics (WCB 2010), 1-6 August 2010, Singapore. http://link.springer.com/chapter/10.1007%2F978-3-642-14515-5_238 doi:10.1007/978-3-642-14515-5_238
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Noor Azmi, Azran Azhim Narita, Y. Muramatsu, K. Morimoto, Y. Tanaka, Masato Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications
description	Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have studied a novel application of microwave radiation to prepare decellularized tissue for tissue-engineered scaffolds. The aim of the present study is to investigate the possibility of microwave effects on decellularization of soft and cartilage tissues. The targeted tissues were decellularized using 2450 MHz of microwave radiation in pulse-circulated 0.9% sodium chloride constituent of 1% sodium deoxycholate solution. To evaluate tissue integrity and cell removal, we used hematoxylin-eosin staining and scanning electron microscope. To investigate the effect of microwave power on targeted sample, we evaluate the radiated microwave distribution in microwave oven using thermograpic camera. Histological results suggest that microwave power influenced the decellularization of samples. It is significantly decellularized, when compared with the other preparation methods. Due to distributions of microwave radiation in the oven are non-uniform, the efficiency of cell removal can be improved by fitting the sample at uniform distribution and higher radiation spot of microwave. In conclusion, combination of microwave radiation and chemically detergent process has potential to decellularize various tissues, even tendon tissue. However, optimization of electromagnetic field in the oven is needed to perform in the further study to improve the decellularization efficiency.
format	Conference or Workshop Item
author	Noor Azmi, Azran Azhim Narita, Y. Muramatsu, K. Morimoto, Y. Tanaka, Masato
author_facet	Noor Azmi, Azran Azhim Narita, Y. Muramatsu, K. Morimoto, Y. Tanaka, Masato
author_sort	Noor Azmi, Azran Azhim
title	Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications
title_short	Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications
title_full	Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications
title_fullStr	Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications
title_full_unstemmed	Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications
title_sort	decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications
publisher	Springer Berlin Heidelberg
publishDate	2010
url	http://irep.iium.edu.my/44078/ http://irep.iium.edu.my/44078/ http://irep.iium.edu.my/44078/ http://irep.iium.edu.my/44078/1/Decellularization_of_Living_Tissue_Using_Microwave_Chemical_Process_for_Tissue-Engineered_Scaffold_Applications.pdf http://irep.iium.edu.my/44078/4/44078.pdf
first_indexed	2023-09-18T21:02:42Z
last_indexed	2023-09-18T21:02:42Z
_version_	1777410729815572480

Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications

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