Development of a module for cell culture based on a decellularized vascular tissue scaffold
Decellularized tissues and organs have been intensively studied in order to use them as a scaffold for proliferation of recipient cells in tissue engineering/regenerative medicine applications. We established a fast and effective method for tissue decellularization by way of shaking a tissue in a de...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
J-STAGE
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/44074/ http://irep.iium.edu.my/44074/ http://irep.iium.edu.my/44074/ http://irep.iium.edu.my/44074/1/Development_of_a_Module_for_Cell_Culture_Based_on_a_Decellularized_Vascular_Tissue_Scaffold.pdf http://irep.iium.edu.my/44074/4/Development_of_a_Module_for_Cell_Culture_Based_on_a_Decellularized_Vascular_Tissue_Scaffold.pdf |
| Summary: | Decellularized tissues and organs have been intensively studied in order to use them as a scaffold for proliferation of recipient cells in tissue engineering/regenerative medicine applications. We established a fast and effective method for tissue decellularization by way of shaking a tissue in a detergent at 300 rpm. In addition, we developed a ring-clamp for fixation of decellularized tissue and created a scaffold module for cell culture by the combination of the ring-clamp and decellularized tissue. The module can be placed in a well of a commercially available 24-well plate. Smooth muscle cells that were seeded on a scaffold module consisting of a decellularized vessel and the ring-clamp showed proliferation with a spindle shape similar to that of vascular smooth muscle cells in the biological body. Vascular endothelial cells that adhered to the scaffold module showed enhanced susceptibility to histamine-induced Ca2+ influx. |
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