The use of sonication treatment to completely decellularize aorta tissue

We have developed a novel sonication decellularization system to prepare completely decellularized bioscaffolds in a short treatment time. The aim of the study is to investigate the sonication decellularization efficiency and its relation with ultrasonic power output and dissolved oxygen (DO) concen...

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Main Authors:	Noor Azmi, Azran Azhim, Yamagami, K., Muramatsu, K., Morimoto, Y., Furukawa, K. S., Tanaka, M., Fukui, Y., Ushida, T.
Format:	Article
Language:	English
Published:	Springer 2013
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/44071/ http://irep.iium.edu.my/44071/ http://irep.iium.edu.my/44071/ http://irep.iium.edu.my/44071/1/The_Use_of_Sonication_Treatment_to_Completely_Decellularize_Aorta_Tissue.pdf

id	iium-44071
recordtype	eprints
spelling	iium-440712015-08-04T01:14:06Z http://irep.iium.edu.my/44071/ The use of sonication treatment to completely decellularize aorta tissue Noor Azmi, Azran Azhim Yamagami, K. Muramatsu, K. Morimoto, Y. Furukawa, K. S. Tanaka, M. Fukui, Y. Ushida, T. TP248.13 Biotechnology We have developed a novel sonication decellularization system to prepare completely decellularized bioscaffolds in a short treatment time. The aim of the study is to investigate the sonication decellularization efficiency and its relation with ultrasonic power output and dissolved oxygen (DO) concentration in different detergent solution. In the study, we used aorta samples to evaluate sonication decellularization efficiency, which assessed treatment duration, washing treatment after decellularization, SDS detergent with/without saline. The treated samples were evaluated histologically by Hematoxylin Eosin (HE) and diamidino-2-phenylindol (DAPI) stains. The concentration of DO was monitored to identify the effect of sonication on cavitation-related DO concentration in the solution. From histological results, the sonication decellularization efficiency was better than the other preparation methods. There were less than 2.0% of DNA remains after sonication decellularization treatment. Decellularization efficiency increased significantly which treated by sonication in SDS without saline as its DO value smaller compared with treatment in SDS with that. In conclusion, we conclude that sonication treatment is capable to use for preparation of the complete decellularized scaffolds in short treatment time Springer 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/44071/1/The_Use_of_Sonication_Treatment_to_Completely_Decellularize_Aorta_Tissue.pdf Noor Azmi, Azran Azhim and Yamagami, K. and Muramatsu, K. and Morimoto, Y. and Furukawa, K. S. and Tanaka, M. and Fukui, Y. and Ushida, T. (2013) The use of sonication treatment to completely decellularize aorta tissue. World Congress on Medical Physics and Biomedical Engineering, 39. pp. 1987-1990. ISSN 1680-0737 http://dx.doi.org/10.1007/978-3-642-29305-4_522 doi:10.1007/978-3-642-29305-4_522
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Noor Azmi, Azran Azhim Yamagami, K. Muramatsu, K. Morimoto, Y. Furukawa, K. S. Tanaka, M. Fukui, Y. Ushida, T. The use of sonication treatment to completely decellularize aorta tissue
description	We have developed a novel sonication decellularization system to prepare completely decellularized bioscaffolds in a short treatment time. The aim of the study is to investigate the sonication decellularization efficiency and its relation with ultrasonic power output and dissolved oxygen (DO) concentration in different detergent solution. In the study, we used aorta samples to evaluate sonication decellularization efficiency, which assessed treatment duration, washing treatment after decellularization, SDS detergent with/without saline. The treated samples were evaluated histologically by Hematoxylin Eosin (HE) and diamidino-2-phenylindol (DAPI) stains. The concentration of DO was monitored to identify the effect of sonication on cavitation-related DO concentration in the solution. From histological results, the sonication decellularization efficiency was better than the other preparation methods. There were less than 2.0% of DNA remains after sonication decellularization treatment. Decellularization efficiency increased significantly which treated by sonication in SDS without saline as its DO value smaller compared with treatment in SDS with that. In conclusion, we conclude that sonication treatment is capable to use for preparation of the complete decellularized scaffolds in short treatment time
format	Article
author	Noor Azmi, Azran Azhim Yamagami, K. Muramatsu, K. Morimoto, Y. Furukawa, K. S. Tanaka, M. Fukui, Y. Ushida, T.
author_facet	Noor Azmi, Azran Azhim Yamagami, K. Muramatsu, K. Morimoto, Y. Furukawa, K. S. Tanaka, M. Fukui, Y. Ushida, T.
author_sort	Noor Azmi, Azran Azhim
title	The use of sonication treatment to completely decellularize aorta tissue
title_short	The use of sonication treatment to completely decellularize aorta tissue
title_full	The use of sonication treatment to completely decellularize aorta tissue
title_fullStr	The use of sonication treatment to completely decellularize aorta tissue
title_full_unstemmed	The use of sonication treatment to completely decellularize aorta tissue
title_sort	use of sonication treatment to completely decellularize aorta tissue
publisher	Springer
publishDate	2013
url	http://irep.iium.edu.my/44071/ http://irep.iium.edu.my/44071/ http://irep.iium.edu.my/44071/ http://irep.iium.edu.my/44071/1/The_Use_of_Sonication_Treatment_to_Completely_Decellularize_Aorta_Tissue.pdf
first_indexed	2023-09-18T21:02:41Z
last_indexed	2023-09-18T21:02:41Z
_version_	1777410728953643008

The use of sonication treatment to completely decellularize aorta tissue

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