Immune response of implanted aortic scaffolds decellularized by sonication treatment

Sonication treatment is used in the preparation of scaffold that was able to elicit minimal immune response following implantation. The aim of this study is to investigate the immunologic response of sonicatedly decellularized aortic scaffolds after 7 days of implantation in rat’s subcutaneous tissu...

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Main Authors:	Syazwani, N., Noor Azmi, Azran Azhim, Morimoto, Y., Furukawa, K., Ushida, T.
Format:	Conference or Workshop Item
Language:	English
Published:	Springer International Publishing 2014
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/43805/ http://irep.iium.edu.my/43805/ http://irep.iium.edu.my/43805/ http://irep.iium.edu.my/43805/1/Immune_Response_of_Implanted_Aortic_Scaffolds_Decellularized_by_Sonication.pdf

id	iium-43805
recordtype	eprints
spelling	iium-438052015-07-15T04:05:49Z http://irep.iium.edu.my/43805/ Immune response of implanted aortic scaffolds decellularized by sonication treatment Syazwani, N. Noor Azmi, Azran Azhim Morimoto, Y. Furukawa, K. Ushida, T. TP248.13 Biotechnology Sonication treatment is used in the preparation of scaffold that was able to elicit minimal immune response following implantation. The aim of this study is to investigate the immunologic response of sonicatedly decellularized aortic scaffolds after 7 days of implantation in rat’s subcutaneous tissue. In this study, aorta samples are sonicatedly decellularized at frequency of 170 kHz in the solution of 0.1% and 2% SDS detergents. It was followed by washing process with PBS solution for 5 days. The treated samples are then evaluated histologically by Hematoxylin Eosin (HE) staining. The samples are subcutaneously implanted on the lower right thoracic cavity of Wistar rats. The implants are explanted and evaluated by HE staining. From the histological results, the implanted samples after sonicatedly decellularized with 2% SDS shows minimal immune response, compared to that decellularized with 0.1% SDS detergents. Hence, treated scaffolds that are sonicatedly decellularized with 2% SDS showed great potential as biomedical implants due to its ability in reducing the immune response to a minimal level. Springer International Publishing 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/43805/1/Immune_Response_of_Implanted_Aortic_Scaffolds_Decellularized_by_Sonication.pdf Syazwani, N. and Noor Azmi, Azran Azhim and Morimoto, Y. and Furukawa, K. and Ushida, T. (2014) Immune response of implanted aortic scaffolds decellularized by sonication treatment. In: The 15th International Conference on Biomedical Engineering IFMBE, 4th to 7th December 2013, Singapore. http://dx.doi.org/10.1007/978-3-319-02913-9_70 doi:10.1007/978-3-319-02913-9_70
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Syazwani, N. Noor Azmi, Azran Azhim Morimoto, Y. Furukawa, K. Ushida, T. Immune response of implanted aortic scaffolds decellularized by sonication treatment
description	Sonication treatment is used in the preparation of scaffold that was able to elicit minimal immune response following implantation. The aim of this study is to investigate the immunologic response of sonicatedly decellularized aortic scaffolds after 7 days of implantation in rat’s subcutaneous tissue. In this study, aorta samples are sonicatedly decellularized at frequency of 170 kHz in the solution of 0.1% and 2% SDS detergents. It was followed by washing process with PBS solution for 5 days. The treated samples are then evaluated histologically by Hematoxylin Eosin (HE) staining. The samples are subcutaneously implanted on the lower right thoracic cavity of Wistar rats. The implants are explanted and evaluated by HE staining. From the histological results, the implanted samples after sonicatedly decellularized with 2% SDS shows minimal immune response, compared to that decellularized with 0.1% SDS detergents. Hence, treated scaffolds that are sonicatedly decellularized with 2% SDS showed great potential as biomedical implants due to its ability in reducing the immune response to a minimal level.
format	Conference or Workshop Item
author	Syazwani, N. Noor Azmi, Azran Azhim Morimoto, Y. Furukawa, K. Ushida, T.
author_facet	Syazwani, N. Noor Azmi, Azran Azhim Morimoto, Y. Furukawa, K. Ushida, T.
author_sort	Syazwani, N.
title	Immune response of implanted aortic scaffolds decellularized by sonication treatment
title_short	Immune response of implanted aortic scaffolds decellularized by sonication treatment
title_full	Immune response of implanted aortic scaffolds decellularized by sonication treatment
title_fullStr	Immune response of implanted aortic scaffolds decellularized by sonication treatment
title_full_unstemmed	Immune response of implanted aortic scaffolds decellularized by sonication treatment
title_sort	immune response of implanted aortic scaffolds decellularized by sonication treatment
publisher	Springer International Publishing
publishDate	2014
url	http://irep.iium.edu.my/43805/ http://irep.iium.edu.my/43805/ http://irep.iium.edu.my/43805/ http://irep.iium.edu.my/43805/1/Immune_Response_of_Implanted_Aortic_Scaffolds_Decellularized_by_Sonication.pdf
first_indexed	2023-09-18T21:02:21Z
last_indexed	2023-09-18T21:02:21Z
_version_	1777410708057620480

Immune response of implanted aortic scaffolds decellularized by sonication treatment

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