Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats
Background Chronic cerebral hypoperfusion has been linked with cognitive decline in aging and Alzheimer's disease (AD). A reduced cerebral blood flow (CBF) is considered to be a predictive marker for the progression into Alzheimer's disease. In order to tackle the neuropathological conse...
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2015
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| Online Access: | http://irep.iium.edu.my/43795/ http://irep.iium.edu.my/43795/ http://irep.iium.edu.my/43795/1/CONFERENCE_2015_Sayyidah.pdf |
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iium-437952016-05-23T05:32:56Z http://irep.iium.edu.my/43795/ Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats Sayeed, Sayyidah Saxena, Anil Kumar Azzubaidi, Marwan Saad A.Talib, Norlelawati QP Physiology R Medicine (General) RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM Therapeutics. Pharmacology Background Chronic cerebral hypoperfusion has been linked with cognitive decline in aging and Alzheimer's disease (AD). A reduced cerebral blood flow (CBF) is considered to be a predictive marker for the progression into Alzheimer's disease. In order to tackle the neuropathological consequences of such a reduction in CBF a similar condition was created in experimental animals. Chronic cerebral hypoperfusion induced neurodegeneration was produced by common carotid artery occlusion (2 vessel occlusion, 2VO) in rats. Since oxidative stress, leading to neuronal apoptosis and death, is one of the mechanisms which is thought to play a significant role in chronic degenerative neurological disorders, the present study was designed to assess the neuroprotective role of Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration. Method After acclimatization, thirty Sprague Dawley rats weighing 200-250 g were equally divided into three groups. Group A – sham control, Group B – 2VO, and Group C – 2VO-E (treated daily with Vitamin E, 100 mg/kg, orally following 2VO). On the 8th week, all the rats were euthanized and the hippocampi were isolated. Viable neuronal cell count in the hippocampal CA-1 region was estimated. Results There was significant difference in neuronal cell death in 2VO group as compared to sham control group. In Vitamin E-treated 2VO (2VO-E) rats, the viable neuronal cell count of the hippocampal CA-1 region was significantly higher as compared to the untreated 2VO group. Conclusion Vitamin E is an effective neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats and can be effectively utilized in the management of AD. The antioxidant activity of Vitamin E is in progress and will be evaluated by the measurement of Isoprostane F2 through Enzyme-Linked Immunosorbent Assay. 2015-06-03 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/43795/1/CONFERENCE_2015_Sayyidah.pdf Sayeed, Sayyidah and Saxena, Anil Kumar and Azzubaidi, Marwan Saad and A.Talib, Norlelawati (2015) Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats. In: International Conference of Antioxidants and Degenerative Diseases (ICADD 2015), 3-4th June 2015, Hotel Istana, Kuala Lumpur, Malaysia. http://icadd.org.my/index.php |
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Digital Repository |
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Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
QP Physiology R Medicine (General) RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM Therapeutics. Pharmacology |
| spellingShingle |
QP Physiology R Medicine (General) RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM Therapeutics. Pharmacology Sayeed, Sayyidah Saxena, Anil Kumar Azzubaidi, Marwan Saad A.Talib, Norlelawati Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats |
| description |
Background
Chronic cerebral hypoperfusion has been linked with cognitive decline in aging and Alzheimer's disease (AD). A reduced cerebral blood flow (CBF) is considered to be a predictive marker for the progression into Alzheimer's disease. In order to tackle the neuropathological consequences of such a reduction in CBF a similar condition was created in experimental animals. Chronic cerebral hypoperfusion induced neurodegeneration was produced by common carotid artery occlusion (2 vessel occlusion, 2VO) in rats. Since oxidative stress, leading to neuronal apoptosis and death, is one of the mechanisms which is thought to play a significant role in chronic
degenerative neurological disorders, the present study was designed to assess the neuroprotective role of Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration.
Method
After acclimatization, thirty Sprague Dawley rats weighing 200-250 g were equally divided into three groups. Group A – sham control, Group B – 2VO, and Group C – 2VO-E (treated daily with Vitamin E, 100 mg/kg, orally following 2VO). On the 8th week, all the rats were euthanized and the hippocampi were isolated. Viable neuronal cell count in the hippocampal CA-1 region was estimated.
Results
There was significant difference in neuronal cell death in 2VO group as compared to sham control group. In Vitamin E-treated 2VO (2VO-E) rats, the viable neuronal cell count of the hippocampal CA-1 region was significantly higher as compared to the untreated 2VO group.
Conclusion
Vitamin E is an effective neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats and can be effectively utilized in the management of AD. The antioxidant activity of Vitamin E is in progress and will be evaluated by the measurement of Isoprostane F2 through Enzyme-Linked Immunosorbent Assay. |
| format |
Conference or Workshop Item |
| author |
Sayeed, Sayyidah Saxena, Anil Kumar Azzubaidi, Marwan Saad A.Talib, Norlelawati |
| author_facet |
Sayeed, Sayyidah Saxena, Anil Kumar Azzubaidi, Marwan Saad A.Talib, Norlelawati |
| author_sort |
Sayeed, Sayyidah |
| title |
Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats |
| title_short |
Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats |
| title_full |
Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats |
| title_fullStr |
Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats |
| title_full_unstemmed |
Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats |
| title_sort |
assessment of ros status and neuroprotection by vitamin e in chronic cerebral hypoperfusion-induced neurodegeneration in rats |
| publishDate |
2015 |
| url |
http://irep.iium.edu.my/43795/ http://irep.iium.edu.my/43795/ http://irep.iium.edu.my/43795/1/CONFERENCE_2015_Sayyidah.pdf |
| first_indexed |
2023-09-18T21:02:21Z |
| last_indexed |
2023-09-18T21:02:21Z |
| _version_ |
1777410707175768064 |