Anti-amoebic properties of carbonyl thiourea derivatives

Thiourea derivatives display a broad spectrum of applications in chemistry, various industries, medicines and various other fields. Recently, different thiourea derivatives have been synthesized and explored for their anti-microbial properties. In this study, four carbonyl thiourea derivatives were...

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Main Authors:	Ibrahim, Maizatul Akma, Mohd Yusof , Mohd Sukeri, Mat Amin, Nakisah
Format:	Article
Language:	English
Published:	MDPI 2014
Subjects:	QD Chemistry QR Microbiology
Online Access:	http://irep.iium.edu.my/43774/ http://irep.iium.edu.my/43774/ http://irep.iium.edu.my/43774/1/Anti-Amoebic_Properties_of_Carbonyl_Thiourea_Derivatives.pdf

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spelling	iium-437742015-07-29T01:20:22Z http://irep.iium.edu.my/43774/ Anti-amoebic properties of carbonyl thiourea derivatives Ibrahim, Maizatul Akma Mohd Yusof , Mohd Sukeri Mat Amin, Nakisah QD Chemistry QR Microbiology Thiourea derivatives display a broad spectrum of applications in chemistry, various industries, medicines and various other fields. Recently, different thiourea derivatives have been synthesized and explored for their anti-microbial properties. In this study, four carbonyl thiourea derivatives were synthesized and characterized, and then further tested for their anti-amoebic properties on two potential pathogenic species of Acanthamoeba, namely A. castellanii (CCAP 1501/2A) and A. polyphaga (CCAP 1501/3A). The results indicate that these newly-synthesized thiourea derivatives are active against both Acanthamoeba species. The IC50 values obtained were in the range of 2.39–8.77 μg·mL-1 (9.47–30.46 μM) for A. castellanii and 3.74–9.30 μg·mL-1 (14.84–31.91 μM) for A. polyphaga. Observations on the amoeba morphology indicated that the compounds caused the reduction of the amoeba size, shortening of their acanthopodia structures, and gave no distinct vacuolar and nuclear structures in the amoeba cells. Meanwhile, fluorescence microscopic observation using acridine orange and propidium iodide (AOPI) staining revealed that the synthesized compounds induced compromised-membrane in the amoeba cells. The results of this study proved that these new carbonyl thiourea derivatives, especially compounds M1 and M2 provide potent cytotoxic properties toward pathogenic Acanthamoeba to suggest that they can be developed as new anti-amoebic agents for the treatment of Acanthamoeba keratitis. MDPI 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/43774/1/Anti-Amoebic_Properties_of_Carbonyl_Thiourea_Derivatives.pdf Ibrahim, Maizatul Akma and Mohd Yusof , Mohd Sukeri and Mat Amin, Nakisah (2014) Anti-amoebic properties of carbonyl thiourea derivatives. Molecules , 19. pp. 5191-5204. ISSN 1420-3049 http://www.mdpi.com/journal/molecules
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry QR Microbiology
spellingShingle	QD Chemistry QR Microbiology Ibrahim, Maizatul Akma Mohd Yusof , Mohd Sukeri Mat Amin, Nakisah Anti-amoebic properties of carbonyl thiourea derivatives
description	Thiourea derivatives display a broad spectrum of applications in chemistry, various industries, medicines and various other fields. Recently, different thiourea derivatives have been synthesized and explored for their anti-microbial properties. In this study, four carbonyl thiourea derivatives were synthesized and characterized, and then further tested for their anti-amoebic properties on two potential pathogenic species of Acanthamoeba, namely A. castellanii (CCAP 1501/2A) and A. polyphaga (CCAP 1501/3A). The results indicate that these newly-synthesized thiourea derivatives are active against both Acanthamoeba species. The IC50 values obtained were in the range of 2.39–8.77 μg·mL-1 (9.47–30.46 μM) for A. castellanii and 3.74–9.30 μg·mL-1 (14.84–31.91 μM) for A. polyphaga. Observations on the amoeba morphology indicated that the compounds caused the reduction of the amoeba size, shortening of their acanthopodia structures, and gave no distinct vacuolar and nuclear structures in the amoeba cells. Meanwhile, fluorescence microscopic observation using acridine orange and propidium iodide (AOPI) staining revealed that the synthesized compounds induced compromised-membrane in the amoeba cells. The results of this study proved that these new carbonyl thiourea derivatives, especially compounds M1 and M2 provide potent cytotoxic properties toward pathogenic Acanthamoeba to suggest that they can be developed as new anti-amoebic agents for the treatment of Acanthamoeba keratitis.
format	Article
author	Ibrahim, Maizatul Akma Mohd Yusof , Mohd Sukeri Mat Amin, Nakisah
author_facet	Ibrahim, Maizatul Akma Mohd Yusof , Mohd Sukeri Mat Amin, Nakisah
author_sort	Ibrahim, Maizatul Akma
title	Anti-amoebic properties of carbonyl thiourea derivatives
title_short	Anti-amoebic properties of carbonyl thiourea derivatives
title_full	Anti-amoebic properties of carbonyl thiourea derivatives
title_fullStr	Anti-amoebic properties of carbonyl thiourea derivatives
title_full_unstemmed	Anti-amoebic properties of carbonyl thiourea derivatives
title_sort	anti-amoebic properties of carbonyl thiourea derivatives
publisher	MDPI
publishDate	2014
url	http://irep.iium.edu.my/43774/ http://irep.iium.edu.my/43774/ http://irep.iium.edu.my/43774/1/Anti-Amoebic_Properties_of_Carbonyl_Thiourea_Derivatives.pdf
first_indexed	2023-09-18T21:02:19Z
last_indexed	2023-09-18T21:02:19Z
_version_	1777410705694130176

Anti-amoebic properties of carbonyl thiourea derivatives

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