Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium
Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). Methods: RNA was extracted from SHED harvested on day 1, 3 and 7. RN...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Piracicaba Dental School, Brazil
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/43699/ http://irep.iium.edu.my/43699/ http://irep.iium.edu.my/43699/1/2015-BJOS-_Expression_analysis_of_Notch_signaling_pathway_molecules_in_SHED.pdf http://irep.iium.edu.my/43699/4/43699_Expression%20analysis%20of%20notch%20signaling%20pathway_SCOPUS.pdf |
| Summary: | Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells
from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely,
keratinocyte growth medium (KGM). Methods: RNA was extracted from SHED harvested on
day 1, 3 and 7. RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR
using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2
and, Hes1) as well as stem cell marker (Nanog). PCR products were electrophoresed on a 2%
agarose gel and stained with SYBR green. Results: Notch-1 was highly expressed in SHED
cultured in KGM and showed increase in density as the days progressed, while Jagged-1
showed a decrease. Jagged-2 on the other hand, showed a slight increase on day 3 followed
by a decrease on day 7. However, Hes-1 was not expressed in SHED cultured in KGM. Nanog
showed expression only on day 3 and gradually increased in expression on day 7.
Conclusions: Notch signaling pathway associated molecules; Notch-1, Jagged-1, Jagged-2,
and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in
the differentiation into epithelial-like cells in human dental pulp tissues.
Keywords: receptors, notch; gene expression; stem cells; tooth, deciduous; culture media. |
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