The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds

A novel decellularization method using sonication treatment is described. Sonication treatment is the combination of physical and chemical agents. These methods will disrupt cell membrane and release cell contents to external environments. The cell removal was facilitated by subsequent rinsing of so...

Full description

Bibliographic Details
Main Authors:	Azhim, A, Syazwani, N, Morimoto, Y, Furukawa, Ks, Ushida, T
Format:	Article
Language:	English
Published:	Sage Publications 2013
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/43658/ http://irep.iium.edu.my/43658/ http://irep.iium.edu.my/43658/ http://irep.iium.edu.my/43658/1/J_Biomater_Appl-2014-Azhim-130-41_print.pdf

id	iium-43658
recordtype	eprints
spelling	iium-436582015-07-14T03:19:39Z http://irep.iium.edu.my/43658/ The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds Azhim, A Syazwani, N Morimoto, Y Furukawa, Ks Ushida, T TP248.13 Biotechnology A novel decellularization method using sonication treatment is described. Sonication treatment is the combination of physical and chemical agents. These methods will disrupt cell membrane and release cell contents to external environments. The cell removal was facilitated by subsequent rinsing of sodium dodecyl sulfate detergents. Sonication treatment is used in the preparation of complete decellularized bioscaffolds. The aim of this study is to confirm the usefulness of sonication treatment for preparation of biological scaffolds. In this study, samples of aortic tissues are decellularized by sonication treatment at frequency of 170 kHz in 0.1% and 2% sodium dodecyl sulfate detergents for 10-h treatment time. The relation between decellularization and sonication parameters such as dissolved oxygen concentration, conductivity, and pH is investigated. Histological analysis and biomechanical testing is performed to evaluate cell removal efficiency as well as changes in biomechanical properties. Minimal inflammation response elicit by bioscaffolds is confirmed by xenogeneic implantation and immunohistochemistry. Sonication treatment is able to produce complete decellularized tissue suggesting that these treatments could be applied widely as one of the decellularization method. Sage Publications 2013-01-02 Article PeerReviewed application/pdf en http://irep.iium.edu.my/43658/1/J_Biomater_Appl-2014-Azhim-130-41_print.pdf Azhim, A and Syazwani, N and Morimoto, Y and Furukawa, Ks and Ushida, T (2013) The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds. Journal of biomaterials applications, 29 (1). pp. 130-141. ISSN 1530-8022 http://www.ncbi.nlm.nih.gov/pubmed/24384523 10.1177/0885328213517579
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Azhim, A Syazwani, N Morimoto, Y Furukawa, Ks Ushida, T The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds
description	A novel decellularization method using sonication treatment is described. Sonication treatment is the combination of physical and chemical agents. These methods will disrupt cell membrane and release cell contents to external environments. The cell removal was facilitated by subsequent rinsing of sodium dodecyl sulfate detergents. Sonication treatment is used in the preparation of complete decellularized bioscaffolds. The aim of this study is to confirm the usefulness of sonication treatment for preparation of biological scaffolds. In this study, samples of aortic tissues are decellularized by sonication treatment at frequency of 170 kHz in 0.1% and 2% sodium dodecyl sulfate detergents for 10-h treatment time. The relation between decellularization and sonication parameters such as dissolved oxygen concentration, conductivity, and pH is investigated. Histological analysis and biomechanical testing is performed to evaluate cell removal efficiency as well as changes in biomechanical properties. Minimal inflammation response elicit by bioscaffolds is confirmed by xenogeneic implantation and immunohistochemistry. Sonication treatment is able to produce complete decellularized tissue suggesting that these treatments could be applied widely as one of the decellularization method.
format	Article
author	Azhim, A Syazwani, N Morimoto, Y Furukawa, Ks Ushida, T
author_facet	Azhim, A Syazwani, N Morimoto, Y Furukawa, Ks Ushida, T
author_sort	Azhim, A
title	The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds
title_short	The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds
title_full	The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds
title_fullStr	The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds
title_full_unstemmed	The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds
title_sort	use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds
publisher	Sage Publications
publishDate	2013
url	http://irep.iium.edu.my/43658/ http://irep.iium.edu.my/43658/ http://irep.iium.edu.my/43658/ http://irep.iium.edu.my/43658/1/J_Biomater_Appl-2014-Azhim-130-41_print.pdf
first_indexed	2023-09-18T21:02:09Z
last_indexed	2023-09-18T21:02:09Z
_version_	1777410695403405312

The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds

Similar Items