Toxicity studies using mammalian cells and impedance spectroscopy method

This research presents a study of cell-based Electric Cell-substrate Impedance Sensing (ECIS) sensors employed for testing toxicants in real-time. Mammalian cells are exposed to toxicants and information about cell viability are obtained from impedance measurements. The toxicants used are nicotine...

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Main Authors:	Xudong, Zhang, Fang, Li, Nordin, Anis Nurashikin, Tarbell, John, Voiculescu, Ioana
Format:	Article
Language:	English English
Published:	Elsevier B.V 2015
Subjects:	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
Online Access:	http://irep.iium.edu.my/43445/ http://irep.iium.edu.my/43445/ http://irep.iium.edu.my/43445/1/1-s2.0-S2214180415000045-main.pdf http://irep.iium.edu.my/43445/4/43445_Toxicity%20studies%20using%20mammalian%20cells%20and%20impedance%20spectroscopy%20method_SCOPUS.pdf

id	iium-43445
recordtype	eprints
spelling	iium-434452017-11-22T01:14:02Z http://irep.iium.edu.my/43445/ Toxicity studies using mammalian cells and impedance spectroscopy method Xudong, Zhang Fang, Li Nordin, Anis Nurashikin Tarbell, John Voiculescu, Ioana TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices This research presents a study of cell-based Electric Cell-substrate Impedance Sensing (ECIS) sensors employed for testing toxicants in real-time. Mammalian cells are exposed to toxicants and information about cell viability are obtained from impedance measurements. The toxicants used are nicotine, phenol, ammonia and aldicarb. Two different adherent mammalian cells lines and cell culturewares were used to optimize the toxicity study. The cell lines used in this study were: bovine aortic endothelial cells (BAEC) and rat fat pad endothelial cells (RFPEC). The BAECs generated higher impedance values and formed cell monolayers at a faster rate compared to RFPECs. The two cell culturewares used in this study were simple open cell-culture wells and enclosed cell culturewares with microfluidic perfusion barriers. Experimental results indicated that the open wells are not suited for toxicity experiments. The turbulent flow created by pipetting media and toxicants in open cell culture wells disrupts the formation of the cell monolayer. When enclosed culture chambers were used, the flow of the cell culture medium and toxicant solutions were laminar on the cell monolayer. Both finite element simulations and experimental results showed that enclosed perfusion chamber have better performance due to the laminar flow of the solutions. The enclosed perfusion chamber also significantly shortens the response time of the cells when exposed to toxicants compared to the open cell culture wells. The experimental results demonstrated that ECIS biosensors seeded with BAECs inside enclosed cell culture chambers successfully assesses the effect of toxicants on mammalian cells in real-time. Elsevier B.V 2015-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/43445/1/1-s2.0-S2214180415000045-main.pdf application/pdf en http://irep.iium.edu.my/43445/4/43445_Toxicity%20studies%20using%20mammalian%20cells%20and%20impedance%20spectroscopy%20method_SCOPUS.pdf Xudong, Zhang and Fang, Li and Nordin, Anis Nurashikin and Tarbell, John and Voiculescu, Ioana (2015) Toxicity studies using mammalian cells and impedance spectroscopy method. Sensing and Bio-Sensing Research, 3. pp. 112-121. ISSN 2214-1804 http://www.sciencedirect.com/science/article/pii/S2214180415000045
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
spellingShingle	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices Xudong, Zhang Fang, Li Nordin, Anis Nurashikin Tarbell, John Voiculescu, Ioana Toxicity studies using mammalian cells and impedance spectroscopy method
description	This research presents a study of cell-based Electric Cell-substrate Impedance Sensing (ECIS) sensors employed for testing toxicants in real-time. Mammalian cells are exposed to toxicants and information about cell viability are obtained from impedance measurements. The toxicants used are nicotine, phenol, ammonia and aldicarb. Two different adherent mammalian cells lines and cell culturewares were used to optimize the toxicity study. The cell lines used in this study were: bovine aortic endothelial cells (BAEC) and rat fat pad endothelial cells (RFPEC). The BAECs generated higher impedance values and formed cell monolayers at a faster rate compared to RFPECs. The two cell culturewares used in this study were simple open cell-culture wells and enclosed cell culturewares with microfluidic perfusion barriers. Experimental results indicated that the open wells are not suited for toxicity experiments. The turbulent flow created by pipetting media and toxicants in open cell culture wells disrupts the formation of the cell monolayer. When enclosed culture chambers were used, the flow of the cell culture medium and toxicant solutions were laminar on the cell monolayer. Both finite element simulations and experimental results showed that enclosed perfusion chamber have better performance due to the laminar flow of the solutions. The enclosed perfusion chamber also significantly shortens the response time of the cells when exposed to toxicants compared to the open cell culture wells. The experimental results demonstrated that ECIS biosensors seeded with BAECs inside enclosed cell culture chambers successfully assesses the effect of toxicants on mammalian cells in real-time.
format	Article
author	Xudong, Zhang Fang, Li Nordin, Anis Nurashikin Tarbell, John Voiculescu, Ioana
author_facet	Xudong, Zhang Fang, Li Nordin, Anis Nurashikin Tarbell, John Voiculescu, Ioana
author_sort	Xudong, Zhang
title	Toxicity studies using mammalian cells and impedance spectroscopy method
title_short	Toxicity studies using mammalian cells and impedance spectroscopy method
title_full	Toxicity studies using mammalian cells and impedance spectroscopy method
title_fullStr	Toxicity studies using mammalian cells and impedance spectroscopy method
title_full_unstemmed	Toxicity studies using mammalian cells and impedance spectroscopy method
title_sort	toxicity studies using mammalian cells and impedance spectroscopy method
publisher	Elsevier B.V
publishDate	2015
url	http://irep.iium.edu.my/43445/ http://irep.iium.edu.my/43445/ http://irep.iium.edu.my/43445/1/1-s2.0-S2214180415000045-main.pdf http://irep.iium.edu.my/43445/4/43445_Toxicity%20studies%20using%20mammalian%20cells%20and%20impedance%20spectroscopy%20method_SCOPUS.pdf
first_indexed	2023-09-18T21:01:53Z
last_indexed	2023-09-18T21:01:53Z
_version_	1777410678547546112

Toxicity studies using mammalian cells and impedance spectroscopy method

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