A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus

Polygalacturonase is one of the important enzymes used in various industries such as food, detergent, pharmaceutical, textile, pulp and paper. A novel liquid/liquid extraction process composed of surfactant and acetonitrile was employed for the first time to purify polygalacturonase from Durio zibet...

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Main Authors:	Amid, Mehrnoush, Manap, Yazid, Azmira, Farhana, Hussina, Muhaini, Sarker, Md. Zaidul Islam
Format:	Article
Language:	English English
Published:	Elsevier B.V. 2015
Subjects:	Q Science (General) T Technology (General)
Online Access:	http://irep.iium.edu.my/43434/ http://irep.iium.edu.my/43434/ http://irep.iium.edu.my/43434/ http://irep.iium.edu.my/43434/1/A_novel_liquid_liquid_extraction_process_composed_of_surfactant_and_acetonitrile.pdf http://irep.iium.edu.my/43434/4/43434_A%20novel%20liquid%20_SCOPUS.pdf

id	iium-43434
recordtype	eprints
spelling	iium-434342017-11-03T04:39:28Z http://irep.iium.edu.my/43434/ A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus Amid, Mehrnoush Manap, Yazid Azmira, Farhana Hussina, Muhaini Sarker, Md. Zaidul Islam Q Science (General) T Technology (General) Polygalacturonase is one of the important enzymes used in various industries such as food, detergent, pharmaceutical, textile, pulp and paper. A novel liquid/liquid extraction process composed of surfactant and acetonitrile was employed for the first time to purify polygalacturonase from Durio zibethinus. The influences of different parameters such as type and concentration of surfactants, concentrations of acetonitrile and composition of surfactant/acetonitrile on partitioning behavior and recovery of polygalacturonase was investigated. Moreover, the effect of pH of system and crude load on purification fold and yield of purified polygalacturonase were studied. The results of the experiment indicated the polygalacturonase was partitioned into surfactant top rich phase with impurities being partitioned into acetonitrile bottom rich phase in the novel method of liquid/liquid process composed of 23% (w/w) Triton X-100 and 19% (w/w) acetonitrile, at 55.6% of TLL (tie line length) crude load of 25% (w/w) at pH 6.0. Recovery and recycling of components also was measured in each successive step of liquid/liquid extraction process. The enzyme was successfully recovered by the method with a high purification factor of 14.3 and yield of 97.3% while phase components were also recovered and recycled above 95%. This study demonstrated that the novel method of liquid/liquid extraction process can be used as an efficient and economical extraction method rather than the traditional methods of extraction for the purification and recovery of the valuable enzyme. Elsevier B.V. 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/43434/1/A_novel_liquid_liquid_extraction_process_composed_of_surfactant_and_acetonitrile.pdf application/pdf en http://irep.iium.edu.my/43434/4/43434_A%20novel%20liquid%20_SCOPUS.pdf Amid, Mehrnoush and Manap, Yazid and Azmira, Farhana and Hussina, Muhaini and Sarker, Md. Zaidul Islam (2015) A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 993-94. pp. 1-8. ISSN 1570-0232 http://www.journals.elsevier.com/journal-of-chromatography-b/ 10.1016/j.jchromb.2015.04.034
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General) T Technology (General)
spellingShingle	Q Science (General) T Technology (General) Amid, Mehrnoush Manap, Yazid Azmira, Farhana Hussina, Muhaini Sarker, Md. Zaidul Islam A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus
description	Polygalacturonase is one of the important enzymes used in various industries such as food, detergent, pharmaceutical, textile, pulp and paper. A novel liquid/liquid extraction process composed of surfactant and acetonitrile was employed for the first time to purify polygalacturonase from Durio zibethinus. The influences of different parameters such as type and concentration of surfactants, concentrations of acetonitrile and composition of surfactant/acetonitrile on partitioning behavior and recovery of polygalacturonase was investigated. Moreover, the effect of pH of system and crude load on purification fold and yield of purified polygalacturonase were studied. The results of the experiment indicated the polygalacturonase was partitioned into surfactant top rich phase with impurities being partitioned into acetonitrile bottom rich phase in the novel method of liquid/liquid process composed of 23% (w/w) Triton X-100 and 19% (w/w) acetonitrile, at 55.6% of TLL (tie line length) crude load of 25% (w/w) at pH 6.0. Recovery and recycling of components also was measured in each successive step of liquid/liquid extraction process. The enzyme was successfully recovered by the method with a high purification factor of 14.3 and yield of 97.3% while phase components were also recovered and recycled above 95%. This study demonstrated that the novel method of liquid/liquid extraction process can be used as an efficient and economical extraction method rather than the traditional methods of extraction for the purification and recovery of the valuable enzyme.
format	Article
author	Amid, Mehrnoush Manap, Yazid Azmira, Farhana Hussina, Muhaini Sarker, Md. Zaidul Islam
author_facet	Amid, Mehrnoush Manap, Yazid Azmira, Farhana Hussina, Muhaini Sarker, Md. Zaidul Islam
author_sort	Amid, Mehrnoush
title	A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus
title_short	A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus
title_full	A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus
title_fullStr	A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus
title_full_unstemmed	A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus
title_sort	novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus
publisher	Elsevier B.V.
publishDate	2015
url	http://irep.iium.edu.my/43434/ http://irep.iium.edu.my/43434/ http://irep.iium.edu.my/43434/ http://irep.iium.edu.my/43434/1/A_novel_liquid_liquid_extraction_process_composed_of_surfactant_and_acetonitrile.pdf http://irep.iium.edu.my/43434/4/43434_A%20novel%20liquid%20_SCOPUS.pdf
first_indexed	2023-09-18T21:01:53Z
last_indexed	2023-09-18T21:01:53Z
_version_	1777410677807251456

A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus

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