Effects of spices and herbal extract on Preadipocyte Cell Differentiation:preliminary results

Type 2 diabetes is multifactorial and polygenic diseases associated with an increased risk of mortality and morbidity. The causes and cure of diabetes are not entirely clear but diet certainly plays an important role in the etiology of type 2 diabetes. Recent research has shown that there is negativ...

Full description

Bibliographic Details
Main Authors:	Azahari, Nuraniza, Bakhtiar, M. Taher, Azahari, Ichwannuraniza, Arief Ichwan, Solachuddin Jauhari, Khattak, Muhammad Muzaffar Ali Khan
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	TX341 Nutrition. Foods and food supply
Online Access:	http://irep.iium.edu.my/4340/ http://irep.iium.edu.my/4340/1/Nuraniza_IRIIE_Poster_Abstract.pdf

id	iium-4340
recordtype	eprints
spelling	iium-43402019-09-03T02:25:44Z http://irep.iium.edu.my/4340/ Effects of spices and herbal extract on Preadipocyte Cell Differentiation:preliminary results Azahari, Nuraniza Bakhtiar, M. Taher Azahari, Ichwannuraniza Arief Ichwan, Solachuddin Jauhari Khattak, Muhammad Muzaffar Ali Khan TX341 Nutrition. Foods and food supply Type 2 diabetes is multifactorial and polygenic diseases associated with an increased risk of mortality and morbidity. The causes and cure of diabetes are not entirely clear but diet certainly plays an important role in the etiology of type 2 diabetes. Recent research has shown that there is negative association between adipokines and prevalence of type 2 diabetes. Therefore, the normal amounts of the adipokines are considered important for the normal functioning of the body (glucose homeostasis). This project was designed to study the effect of selected Malaysian spices and herbs (based on the traditional use in the treatment of type 2 diabetes). The spices and herbs namely Syzgium colyanthum, Peronema canescens, Orthosiphon stamineus, Lagerostroemia speciosa, Momordica charantia, Tinospara crispa, Pithecellobium jiringa, Cinnamomum zeylanicum, and Andrographis paniculata were procured through local supplier and water extracts were obtained and freeze dried. The extracts effects were studied on cell growth using an in-vitro model of 3T3-L1 adipocyte cell line. The preliminary test carried out was to know the effect of the listed extracts to induce changes from preadipocyte to adipocyte. This test was performed by using a mixture of dexamethasone, 1-isobutyl-3-methylxantine (IBMX) and the extracts from the aforementioned spices and herbs. Preliminary results indicate that among the extracts used, Orthosiphon stamineus and Lagerostroemia speciosa had stronger activity/effect on preadipocyte differentiation. This is of clinical importance both for type 2 diabetes as well as for obesity. The extracts from the same sources will be incorporated to preadipocytes culture for further tests including protein analysis, adipogenesis and adipolysis assays using appropriate kits. Lastly, the adipocytes will be assessed for adiponectin and leptin protein concentration using competitive colorimetric enzyme-linked immunosorbent assay (ELISA) kit known as the B-bridge adiponectin ELISA kit method. Preliminary results indicate that Orthosiphon stamineus and Lagerostroemia speciosa induce cell differentiation in preadipocytes which will be further investigated. 2011 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/4340/1/Nuraniza_IRIIE_Poster_Abstract.pdf Azahari, Nuraniza and Bakhtiar, M. Taher and Azahari, Ichwannuraniza and Arief Ichwan, Solachuddin Jauhari and Khattak, Muhammad Muzaffar Ali Khan (2011) Effects of spices and herbal extract on Preadipocyte Cell Differentiation:preliminary results. In: IIUM Research, Invention and Innovation Exhibition (IRIIE) 2011, 9-10 February 2011, IIUM, Kuala Lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TX341 Nutrition. Foods and food supply
spellingShingle	TX341 Nutrition. Foods and food supply Azahari, Nuraniza Bakhtiar, M. Taher Azahari, Ichwannuraniza Arief Ichwan, Solachuddin Jauhari Khattak, Muhammad Muzaffar Ali Khan Effects of spices and herbal extract on Preadipocyte Cell Differentiation:preliminary results
description	Type 2 diabetes is multifactorial and polygenic diseases associated with an increased risk of mortality and morbidity. The causes and cure of diabetes are not entirely clear but diet certainly plays an important role in the etiology of type 2 diabetes. Recent research has shown that there is negative association between adipokines and prevalence of type 2 diabetes. Therefore, the normal amounts of the adipokines are considered important for the normal functioning of the body (glucose homeostasis). This project was designed to study the effect of selected Malaysian spices and herbs (based on the traditional use in the treatment of type 2 diabetes). The spices and herbs namely Syzgium colyanthum, Peronema canescens, Orthosiphon stamineus, Lagerostroemia speciosa, Momordica charantia, Tinospara crispa, Pithecellobium jiringa, Cinnamomum zeylanicum, and Andrographis paniculata were procured through local supplier and water extracts were obtained and freeze dried. The extracts effects were studied on cell growth using an in-vitro model of 3T3-L1 adipocyte cell line. The preliminary test carried out was to know the effect of the listed extracts to induce changes from preadipocyte to adipocyte. This test was performed by using a mixture of dexamethasone, 1-isobutyl-3-methylxantine (IBMX) and the extracts from the aforementioned spices and herbs. Preliminary results indicate that among the extracts used, Orthosiphon stamineus and Lagerostroemia speciosa had stronger activity/effect on preadipocyte differentiation. This is of clinical importance both for type 2 diabetes as well as for obesity. The extracts from the same sources will be incorporated to preadipocytes culture for further tests including protein analysis, adipogenesis and adipolysis assays using appropriate kits. Lastly, the adipocytes will be assessed for adiponectin and leptin protein concentration using competitive colorimetric enzyme-linked immunosorbent assay (ELISA) kit known as the B-bridge adiponectin ELISA kit method. Preliminary results indicate that Orthosiphon stamineus and Lagerostroemia speciosa induce cell differentiation in preadipocytes which will be further investigated.
format	Conference or Workshop Item
author	Azahari, Nuraniza Bakhtiar, M. Taher Azahari, Ichwannuraniza Arief Ichwan, Solachuddin Jauhari Khattak, Muhammad Muzaffar Ali Khan
author_facet	Azahari, Nuraniza Bakhtiar, M. Taher Azahari, Ichwannuraniza Arief Ichwan, Solachuddin Jauhari Khattak, Muhammad Muzaffar Ali Khan
author_sort	Azahari, Nuraniza
title	Effects of spices and herbal extract on Preadipocyte Cell Differentiation:preliminary results
title_short	Effects of spices and herbal extract on Preadipocyte Cell Differentiation:preliminary results
title_full	Effects of spices and herbal extract on Preadipocyte Cell Differentiation:preliminary results
title_fullStr	Effects of spices and herbal extract on Preadipocyte Cell Differentiation:preliminary results
title_full_unstemmed	Effects of spices and herbal extract on Preadipocyte Cell Differentiation:preliminary results
title_sort	effects of spices and herbal extract on preadipocyte cell differentiation:preliminary results
publishDate	2011
url	http://irep.iium.edu.my/4340/ http://irep.iium.edu.my/4340/1/Nuraniza_IRIIE_Poster_Abstract.pdf
first_indexed	2023-09-18T20:12:31Z
last_indexed	2023-09-18T20:12:31Z
_version_	1777407572074037248

Effects of spices and herbal extract on Preadipocyte Cell Differentiation:preliminary results

Similar Items