Case Study: Recombinant Bromelain Downstream Processing

This chapter presents details on the purification, formulation and drying of recombinant bromelain. The wide range of applications of recombinant bromelain has increased interest in finding viable purification techniques for large-scale production. An affinity chromatography technique was developed...

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Main Authors:	Amid, Azura, Mohd Arshad, Zatul Iffah, Othman, Muhd. Ezza Faiez
Format:	Book Chapter
Language:	English English
Published:	Springer International Publishing 2015
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/43384/ http://irep.iium.edu.my/43384/1/Case_study-_recombinant_bromelain_downstream_processing-_chapter_12.pdf http://irep.iium.edu.my/43384/3/Recombinant_Enzymes_TOC.pdf

id	iium-43384
recordtype	eprints
spelling	iium-433842015-07-31T02:41:03Z http://irep.iium.edu.my/43384/ Case Study: Recombinant Bromelain Downstream Processing Amid, Azura Mohd Arshad, Zatul Iffah Othman, Muhd. Ezza Faiez TP248.13 Biotechnology This chapter presents details on the purification, formulation and drying of recombinant bromelain. The wide range of applications of recombinant bromelain has increased interest in finding viable purification techniques for large-scale production. An affinity chromatography technique was developed by Amid and co-workers (Expression, purification, and characterization of a recombinant stem bromelain from Ananas comosus, Process Biochem 46:2232–2239, 2011) to purify recombinant bromelain. However, this technique presented low recovery and small sample loading capacity and thus is not suitable as a purification tool in the largescale production of recombinant bromelain. An aqueous two-phase system is one alternative method that we use to purify recombinant bromelain, as it reliable and easy to scale up and has a low cost. As part of avoiding cysteine degradation, spray drying the purified recombinant protein with maltodextrin as an excipient provides the possibility of preserving its activity and creating fine particles that are suitable for end-product application. The processes of the purification, formulation and spray drying of recombinant bromelain are explained briefly. Springer International Publishing Amid, Azura 2015 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/43384/1/Case_study-_recombinant_bromelain_downstream_processing-_chapter_12.pdf application/pdf en http://irep.iium.edu.my/43384/3/Recombinant_Enzymes_TOC.pdf Amid, Azura and Mohd Arshad, Zatul Iffah and Othman, Muhd. Ezza Faiez (2015) Case Study: Recombinant Bromelain Downstream Processing. In: Recombinant Enzymes—From Basic Science to Commercialization. Springer International Publishing, Cham Heidelberg New York Dordrecht London, pp. 175-185. ISBN 978-3-319-12396-7
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Amid, Azura Mohd Arshad, Zatul Iffah Othman, Muhd. Ezza Faiez Case Study: Recombinant Bromelain Downstream Processing
description	This chapter presents details on the purification, formulation and drying of recombinant bromelain. The wide range of applications of recombinant bromelain has increased interest in finding viable purification techniques for large-scale production. An affinity chromatography technique was developed by Amid and co-workers (Expression, purification, and characterization of a recombinant stem bromelain from Ananas comosus, Process Biochem 46:2232–2239, 2011) to purify recombinant bromelain. However, this technique presented low recovery and small sample loading capacity and thus is not suitable as a purification tool in the largescale production of recombinant bromelain. An aqueous two-phase system is one alternative method that we use to purify recombinant bromelain, as it reliable and easy to scale up and has a low cost. As part of avoiding cysteine degradation, spray drying the purified recombinant protein with maltodextrin as an excipient provides the possibility of preserving its activity and creating fine particles that are suitable for end-product application. The processes of the purification, formulation and spray drying of recombinant bromelain are explained briefly.
author2	Amid, Azura
author_facet	Amid, Azura Amid, Azura Mohd Arshad, Zatul Iffah Othman, Muhd. Ezza Faiez
format	Book Chapter
author	Amid, Azura Mohd Arshad, Zatul Iffah Othman, Muhd. Ezza Faiez
author_sort	Amid, Azura
title	Case Study: Recombinant Bromelain Downstream Processing
title_short	Case Study: Recombinant Bromelain Downstream Processing
title_full	Case Study: Recombinant Bromelain Downstream Processing
title_fullStr	Case Study: Recombinant Bromelain Downstream Processing
title_full_unstemmed	Case Study: Recombinant Bromelain Downstream Processing
title_sort	case study: recombinant bromelain downstream processing
publisher	Springer International Publishing
publishDate	2015
url	http://irep.iium.edu.my/43384/ http://irep.iium.edu.my/43384/1/Case_study-_recombinant_bromelain_downstream_processing-_chapter_12.pdf http://irep.iium.edu.my/43384/3/Recombinant_Enzymes_TOC.pdf
first_indexed	2023-09-18T21:01:49Z
last_indexed	2023-09-18T21:01:49Z
_version_	1777410673811128320

Case Study: Recombinant Bromelain Downstream Processing

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