Case study: recombinant Bromelain cloning, characterization and upstream processes
This chapter presents the recombinant stem bromelain cloning procedure followed by its characterization and finally upstream processing. Most of the procedures had patents filed in Malaysia, Europe and the United State of America with application numbers PI 20095434, 10015711.4, and 12968766, respe...
| Main Authors: | , , |
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| Format: | Book Chapter |
| Language: | English English |
| Published: |
Springer International Publishing
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/43383/ http://irep.iium.edu.my/43383/1/Case_study-recombinant_bromelain_cloning-chapter_11.pdf http://irep.iium.edu.my/43383/3/Recombinant_Enzymes_TOC.pdf |
| Summary: | This chapter presents the recombinant stem bromelain cloning procedure followed by its characterization and finally upstream processing. Most of the procedures
had patents filed in Malaysia, Europe and the United State of America with application numbers PI 20095434, 10015711.4, and 12968766, respectively. Portions
of the experimental procedures were also presented in our publication (Amid et al., Process Biochem 46:2232–2239, 2011). The entire cloning process, characterization
and the upstream processing of the recombinant bromelain production are briefly explained. |
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