New lipase producing β-proteobacteria strains caldimonas sp. and tepidimonas sp. isolated from a Malaysian hot springs

Microbial lipolytic enzymes have attracted considerable attention owing to their biotechnological potential. In this study, thermophilic bacteria producing lipase were isolated from Bentong and Sungai Lembing hot springs, in Pahang, Malaysia. Out of 25 colonies isolated, 14 samples showed to produce...

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Main Authors:	Mohd ghazali, Sharul Azim, Tengku Abdul Hamid, Tengku Haziyamin
Format:	Article
Language:	English
Published:	Penerbit UKM 2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/43375/ http://irep.iium.edu.my/43375/ http://irep.iium.edu.my/43375/ http://irep.iium.edu.my/43375/1/09_Sharul_Azim_Mohd_Ghazali.pdf

id	iium-43375
recordtype	eprints
spelling	iium-433752017-08-08T03:42:24Z http://irep.iium.edu.my/43375/ New lipase producing β-proteobacteria strains caldimonas sp. and tepidimonas sp. isolated from a Malaysian hot springs Mohd ghazali, Sharul Azim Tengku Abdul Hamid, Tengku Haziyamin Q Science (General) Microbial lipolytic enzymes have attracted considerable attention owing to their biotechnological potential. In this study, thermophilic bacteria producing lipase were isolated from Bentong and Sungai Lembing hot springs, in Pahang, Malaysia. Out of 25 colonies isolated, 14 samples showed to produce clear zones surrounding the growth on tributyrin and trioelin agar plates. All 14 isolates showed Gram-negative bacteria with short rod morphology. PCR amplification of 16S ribosomal DNA gene showed that these isolates were clustered with subclass β-proteobacteria consisting of thermophilic bacteria that produce lipase. Phylogenetic analysis was carried out with the highly similar species and four isolates (SglA1, BtnC1, BtnC2 and BtnC3) are related to genus Caldimonas and 10 isolates (SglB1, SglB2, SglB3, SglB4, BtnB1, BtnB2 BtnD1, BtnD2, BtnD3 and BtnD4) belonged to genus Tepidimonas. These results indicated that novel lipase-producing thermophilic β-proteobacteria could be isolated from these hot springs Penerbit UKM 2015-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/43375/1/09_Sharul_Azim_Mohd_Ghazali.pdf Mohd ghazali, Sharul Azim and Tengku Abdul Hamid, Tengku Haziyamin (2015) New lipase producing β-proteobacteria strains caldimonas sp. and tepidimonas sp. isolated from a Malaysian hot springs. Sains Malaysiana, 44 (5). pp. 701-706. ISSN 0126-6039 http://www.ukm.my/jsm/ http://www.ukm.my/jsm/english_journals/vol44num5_2015/contentsVol44num5_2015.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Mohd ghazali, Sharul Azim Tengku Abdul Hamid, Tengku Haziyamin New lipase producing β-proteobacteria strains caldimonas sp. and tepidimonas sp. isolated from a Malaysian hot springs
description	Microbial lipolytic enzymes have attracted considerable attention owing to their biotechnological potential. In this study, thermophilic bacteria producing lipase were isolated from Bentong and Sungai Lembing hot springs, in Pahang, Malaysia. Out of 25 colonies isolated, 14 samples showed to produce clear zones surrounding the growth on tributyrin and trioelin agar plates. All 14 isolates showed Gram-negative bacteria with short rod morphology. PCR amplification of 16S ribosomal DNA gene showed that these isolates were clustered with subclass β-proteobacteria consisting of thermophilic bacteria that produce lipase. Phylogenetic analysis was carried out with the highly similar species and four isolates (SglA1, BtnC1, BtnC2 and BtnC3) are related to genus Caldimonas and 10 isolates (SglB1, SglB2, SglB3, SglB4, BtnB1, BtnB2 BtnD1, BtnD2, BtnD3 and BtnD4) belonged to genus Tepidimonas. These results indicated that novel lipase-producing thermophilic β-proteobacteria could be isolated from these hot springs
format	Article
author	Mohd ghazali, Sharul Azim Tengku Abdul Hamid, Tengku Haziyamin
author_facet	Mohd ghazali, Sharul Azim Tengku Abdul Hamid, Tengku Haziyamin
author_sort	Mohd ghazali, Sharul Azim
title	New lipase producing β-proteobacteria strains caldimonas sp. and tepidimonas sp. isolated from a Malaysian hot springs
title_short	New lipase producing β-proteobacteria strains caldimonas sp. and tepidimonas sp. isolated from a Malaysian hot springs
title_full	New lipase producing β-proteobacteria strains caldimonas sp. and tepidimonas sp. isolated from a Malaysian hot springs
title_fullStr	New lipase producing β-proteobacteria strains caldimonas sp. and tepidimonas sp. isolated from a Malaysian hot springs
title_full_unstemmed	New lipase producing β-proteobacteria strains caldimonas sp. and tepidimonas sp. isolated from a Malaysian hot springs
title_sort	new lipase producing β-proteobacteria strains caldimonas sp. and tepidimonas sp. isolated from a malaysian hot springs
publisher	Penerbit UKM
publishDate	2015
url	http://irep.iium.edu.my/43375/ http://irep.iium.edu.my/43375/ http://irep.iium.edu.my/43375/ http://irep.iium.edu.my/43375/1/09_Sharul_Azim_Mohd_Ghazali.pdf
first_indexed	2023-09-18T21:01:48Z
last_indexed	2023-09-18T21:01:48Z
_version_	1777410672518234112

New lipase producing β-proteobacteria strains caldimonas sp. and tepidimonas sp. isolated from a Malaysian hot springs

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