The phytochemical contents and antimicrobial activities of Malaysian Calophyllum rubiginosum

Problem statement: Many species of plants in Malaysia are widely used in folk medicine. However, Calophyllum species have been used in traditional medicine for their therapeutic values for many years. Several studies reported that antimicrobial, antifungal, anti-HIV and anti-cancer compounds were...

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Main Authors:	ALkhamaiseh, Suhaib Ibrahim, Bakhtiar, M. Taher, Ahmad, Farediah
Format:	Article
Language:	English
Published:	Science Publications 2011
Subjects:	QK Botany RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/4327/ http://irep.iium.edu.my/4327/ http://irep.iium.edu.my/4327/1/ajassp.2011.201.205-Suhaib.pdf

id	iium-4327
recordtype	eprints
spelling	iium-43272012-02-29T07:01:50Z http://irep.iium.edu.my/4327/ The phytochemical contents and antimicrobial activities of Malaysian Calophyllum rubiginosum ALkhamaiseh, Suhaib Ibrahim Bakhtiar, M. Taher Ahmad, Farediah QK Botany RS Pharmacy and materia medica Problem statement: Many species of plants in Malaysia are widely used in folk medicine. However, Calophyllum species have been used in traditional medicine for their therapeutic values for many years. Several studies reported that antimicrobial, antifungal, anti-HIV and anti-cancer compounds were isolated from numerous Calophyllum species. Approach: The stem bark was extracted by EtOH, after with it was fractionated with n-Hexane, Dichloromethane (DCM) and MeOH by using vacuum liquid chromatography apparatus. Phytochemical contents were examined to evaluate the phinolic, flavonoid and flavonol contents. Also the antimicrobial activity was carried out by using disc diffusion and dilution method to evaluate antimicrobial activity of the crude and the fractions respectively. Six references microbial strains of human pathogens were used for examined the anti microbial activity. The two Gram-positive bacteria (Staphylococcus aureus, Bacillus cereus) and two Gram-negative (Pseudomonas aeruginosa, Escherichia coli) were used for antibacterial test. Also two fungal strains (Candida albicans and Cryptococcus neoformans) were used for antifungal test. Results: Although the C. rubiginosum has phytochemicals as all plants, but it was showed a high content of flavonol in the range of 11.9- 15.2 μg mL−1. The C. rubiginosum fractions were showed no activity against gram negative and fungus. However, the non polar and semi polar fractions were showed a result MIC 12.5 μg mL−1 against B.cereus bacteria. While the MeOH fraction indicated for low or no activity against bacteria and fungus. Conclusion: At last, the optimistic result of this study encourage us to go forward for further studies in the future to isolate the active compound of the stem bark of C. rubiginosum, where it could lead to a new antibiotic, whereas this species never investigated before. Science Publications 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/4327/1/ajassp.2011.201.205-Suhaib.pdf ALkhamaiseh, Suhaib Ibrahim and Bakhtiar, M. Taher and Ahmad, Farediah (2011) The phytochemical contents and antimicrobial activities of Malaysian Calophyllum rubiginosum. American Journal of Applied Sciences, 8 (3). pp. 201-205. ISSN 1546-9239 http://thescipub.com/ajas.toc
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QK Botany RS Pharmacy and materia medica
spellingShingle	QK Botany RS Pharmacy and materia medica ALkhamaiseh, Suhaib Ibrahim Bakhtiar, M. Taher Ahmad, Farediah The phytochemical contents and antimicrobial activities of Malaysian Calophyllum rubiginosum
description	Problem statement: Many species of plants in Malaysia are widely used in folk medicine. However, Calophyllum species have been used in traditional medicine for their therapeutic values for many years. Several studies reported that antimicrobial, antifungal, anti-HIV and anti-cancer compounds were isolated from numerous Calophyllum species. Approach: The stem bark was extracted by EtOH, after with it was fractionated with n-Hexane, Dichloromethane (DCM) and MeOH by using vacuum liquid chromatography apparatus. Phytochemical contents were examined to evaluate the phinolic, flavonoid and flavonol contents. Also the antimicrobial activity was carried out by using disc diffusion and dilution method to evaluate antimicrobial activity of the crude and the fractions respectively. Six references microbial strains of human pathogens were used for examined the anti microbial activity. The two Gram-positive bacteria (Staphylococcus aureus, Bacillus cereus) and two Gram-negative (Pseudomonas aeruginosa, Escherichia coli) were used for antibacterial test. Also two fungal strains (Candida albicans and Cryptococcus neoformans) were used for antifungal test. Results: Although the C. rubiginosum has phytochemicals as all plants, but it was showed a high content of flavonol in the range of 11.9- 15.2 μg mL−1. The C. rubiginosum fractions were showed no activity against gram negative and fungus. However, the non polar and semi polar fractions were showed a result MIC 12.5 μg mL−1 against B.cereus bacteria. While the MeOH fraction indicated for low or no activity against bacteria and fungus. Conclusion: At last, the optimistic result of this study encourage us to go forward for further studies in the future to isolate the active compound of the stem bark of C. rubiginosum, where it could lead to a new antibiotic, whereas this species never investigated before.
format	Article
author	ALkhamaiseh, Suhaib Ibrahim Bakhtiar, M. Taher Ahmad, Farediah
author_facet	ALkhamaiseh, Suhaib Ibrahim Bakhtiar, M. Taher Ahmad, Farediah
author_sort	ALkhamaiseh, Suhaib Ibrahim
title	The phytochemical contents and antimicrobial activities of Malaysian Calophyllum rubiginosum
title_short	The phytochemical contents and antimicrobial activities of Malaysian Calophyllum rubiginosum
title_full	The phytochemical contents and antimicrobial activities of Malaysian Calophyllum rubiginosum
title_fullStr	The phytochemical contents and antimicrobial activities of Malaysian Calophyllum rubiginosum
title_full_unstemmed	The phytochemical contents and antimicrobial activities of Malaysian Calophyllum rubiginosum
title_sort	phytochemical contents and antimicrobial activities of malaysian calophyllum rubiginosum
publisher	Science Publications
publishDate	2011
url	http://irep.iium.edu.my/4327/ http://irep.iium.edu.my/4327/ http://irep.iium.edu.my/4327/1/ajassp.2011.201.205-Suhaib.pdf
first_indexed	2023-09-18T20:12:30Z
last_indexed	2023-09-18T20:12:30Z
_version_	1777407571336888320

The phytochemical contents and antimicrobial activities of Malaysian Calophyllum rubiginosum

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