In-vitro anti-microbial and brine-shrimp lethality potential of the leaves extract of nahar (Mesua ferrea) plant

The growing interest in human health and increasing bacterial resistance to antibiotics among other reasons have resulted into an increasing need for the exploration of both the essential oils and other plant extracts in the food and pharmaceutical industries. This work, as part of on-going work o...

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Main Authors:	Adewale, Ahmed Idris, Mirghani, Mohamed Elwathig Saeed, Muyibi, Suleyman Aremu, Daoud, Jamal Ibrahim, Abimbola, Mikail Maryam
Format:	Conference or Workshop Item
Language:	English English English
Published:	2011
Subjects:	Q Science (General) TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/4313/ http://irep.iium.edu.my/4313/ http://irep.iium.edu.my/4313/2/ICBioE41.pdf http://irep.iium.edu.my/4313/3/In-vitro_Anti-Microbial_%28Abstract%29.pdf http://irep.iium.edu.my/4313/9/In-vitro_Anti-Microbial_and_Brine-Shrimp.pdf

id	iium-4313
recordtype	eprints
spelling	iium-43132012-01-18T08:34:50Z http://irep.iium.edu.my/4313/ In-vitro anti-microbial and brine-shrimp lethality potential of the leaves extract of nahar (Mesua ferrea) plant Adewale, Ahmed Idris Mirghani, Mohamed Elwathig Saeed Muyibi, Suleyman Aremu Daoud, Jamal Ibrahim Abimbola, Mikail Maryam Q Science (General) TP248.13 Biotechnology The growing interest in human health and increasing bacterial resistance to antibiotics among other reasons have resulted into an increasing need for the exploration of both the essential oils and other plant extracts in the food and pharmaceutical industries. This work, as part of on-going work on the leaves of Nahar (Mesua ferrea) plant, was aimed at evaluating the antimicrobial activity, minimum inhibitory concentration as well as the cytotoxicity of the leaves extract. The dry leaves were grinded and extracted in an oven shaker set at 37°C and 200rpm for 24 hours using ethanol and methanol as solvents. The agar disc diffusion method was used for the evaluation of antibacterial property of the leaves extract, micro broth dilution was employed for the determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), while Brine shrimp (Artemia salina) lethality bioassay was made use of for the cytotoxicity assay. Ethanol gave higher extract’s yield (6.20%) than methanol. The extract showed a remarkable antibacterial property against all the selected microbes (Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus) with the inhibition zones ranging from 16.0±0.5mm to 18.0±0.5mm for all the tested bacteria. The MIC range of 2.5- 0.625 mg/ml with MBC value of 5mg/ml was obtained for the gram-negative bacteria while MIC rangeof 1.3- 0.313mg/ml with MBC value of 2.5mg/ml was obtained for the gram-positive bacteria. The leaves extract was found to be toxic to the Brine shrimps with LC50 of 500ppm (μg/ml) suggesting that the extracts may contain bioactive compounds of potential the therapeutic and prophylactic significance. 2011-05-17 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/4313/2/ICBioE41.pdf application/pdf en http://irep.iium.edu.my/4313/3/In-vitro_Anti-Microbial_%28Abstract%29.pdf application/pdf en http://irep.iium.edu.my/4313/9/In-vitro_Anti-Microbial_and_Brine-Shrimp.pdf Adewale, Ahmed Idris and Mirghani, Mohamed Elwathig Saeed and Muyibi, Suleyman Aremu and Daoud, Jamal Ibrahim and Abimbola, Mikail Maryam (2011) In-vitro anti-microbial and brine-shrimp lethality potential of the leaves extract of nahar (Mesua ferrea) plant. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	Q Science (General) TP248.13 Biotechnology
spellingShingle	Q Science (General) TP248.13 Biotechnology Adewale, Ahmed Idris Mirghani, Mohamed Elwathig Saeed Muyibi, Suleyman Aremu Daoud, Jamal Ibrahim Abimbola, Mikail Maryam In-vitro anti-microbial and brine-shrimp lethality potential of the leaves extract of nahar (Mesua ferrea) plant
description	The growing interest in human health and increasing bacterial resistance to antibiotics among other reasons have resulted into an increasing need for the exploration of both the essential oils and other plant extracts in the food and pharmaceutical industries. This work, as part of on-going work on the leaves of Nahar (Mesua ferrea) plant, was aimed at evaluating the antimicrobial activity, minimum inhibitory concentration as well as the cytotoxicity of the leaves extract. The dry leaves were grinded and extracted in an oven shaker set at 37°C and 200rpm for 24 hours using ethanol and methanol as solvents. The agar disc diffusion method was used for the evaluation of antibacterial property of the leaves extract, micro broth dilution was employed for the determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), while Brine shrimp (Artemia salina) lethality bioassay was made use of for the cytotoxicity assay. Ethanol gave higher extract’s yield (6.20%) than methanol. The extract showed a remarkable antibacterial property against all the selected microbes (Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus) with the inhibition zones ranging from 16.0±0.5mm to 18.0±0.5mm for all the tested bacteria. The MIC range of 2.5- 0.625 mg/ml with MBC value of 5mg/ml was obtained for the gram-negative bacteria while MIC rangeof 1.3- 0.313mg/ml with MBC value of 2.5mg/ml was obtained for the gram-positive bacteria. The leaves extract was found to be toxic to the Brine shrimps with LC50 of 500ppm (μg/ml) suggesting that the extracts may contain bioactive compounds of potential the therapeutic and prophylactic significance.
format	Conference or Workshop Item
author	Adewale, Ahmed Idris Mirghani, Mohamed Elwathig Saeed Muyibi, Suleyman Aremu Daoud, Jamal Ibrahim Abimbola, Mikail Maryam
author_facet	Adewale, Ahmed Idris Mirghani, Mohamed Elwathig Saeed Muyibi, Suleyman Aremu Daoud, Jamal Ibrahim Abimbola, Mikail Maryam
author_sort	Adewale, Ahmed Idris
title	In-vitro anti-microbial and brine-shrimp lethality potential of the leaves extract of nahar (Mesua ferrea) plant
title_short	In-vitro anti-microbial and brine-shrimp lethality potential of the leaves extract of nahar (Mesua ferrea) plant
title_full	In-vitro anti-microbial and brine-shrimp lethality potential of the leaves extract of nahar (Mesua ferrea) plant
title_fullStr	In-vitro anti-microbial and brine-shrimp lethality potential of the leaves extract of nahar (Mesua ferrea) plant
title_full_unstemmed	In-vitro anti-microbial and brine-shrimp lethality potential of the leaves extract of nahar (Mesua ferrea) plant
title_sort	in-vitro anti-microbial and brine-shrimp lethality potential of the leaves extract of nahar (mesua ferrea) plant
publishDate	2011
url	http://irep.iium.edu.my/4313/ http://irep.iium.edu.my/4313/ http://irep.iium.edu.my/4313/2/ICBioE41.pdf http://irep.iium.edu.my/4313/3/In-vitro_Anti-Microbial_%28Abstract%29.pdf http://irep.iium.edu.my/4313/9/In-vitro_Anti-Microbial_and_Brine-Shrimp.pdf
first_indexed	2023-09-18T20:12:29Z
last_indexed	2023-09-18T20:12:29Z
_version_	1777407570465521664

In-vitro anti-microbial and brine-shrimp lethality potential of the leaves extract of nahar (Mesua ferrea) plant

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