Optimization of transfection of human coxsackie and adenovirus receptor into mammalian cells mediated by liposomes-base gene delivery

Cationic liposome-based reagents provide reliable and simple protocol for in vitro transfection in a broad range of mammalian cell types. High transfection efficacies and low cytotoxicity of a liposome-based transfection reagent was required to retain adequate viability and high level of transgene...

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Main Authors:	Baba, Sayang, Elengoe, Asita, Hussein, Abdul Rahim, Sugeng , Triwahyono, Draman, Samsul, Hamdan, Salehhuddin
Format:	Article
Language:	English English
Published:	Life Science Journal 2015
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/43121/ http://irep.iium.edu.my/43121/ http://irep.iium.edu.my/43121/ http://irep.iium.edu.my/43121/1/Optimization_of_transfection_of_human.pdf http://irep.iium.edu.my/43121/2/cover_Life_Science_Journal.pdf

id	iium-43121
recordtype	eprints
spelling	iium-431212015-05-26T02:37:36Z http://irep.iium.edu.my/43121/ Optimization of transfection of human coxsackie and adenovirus receptor into mammalian cells mediated by liposomes-base gene delivery Baba, Sayang Elengoe, Asita Hussein, Abdul Rahim Sugeng , Triwahyono Draman, Samsul Hamdan, Salehhuddin R Medicine (General) Cationic liposome-based reagents provide reliable and simple protocol for in vitro transfection in a broad range of mammalian cell types. High transfection efficacies and low cytotoxicity of a liposome-based transfection reagent was required to retain adequate viability and high level of transgene of transfected Chinese hamster ovary (CHO) cells for subsequent assay. Three commercial liposome-based transfection reagents were utilized and compared for their aptitude to transfect in-vitro CHO cells with reporter gene expressing the red fluorescent (pDsRed-N1) and fusion pDsRed-N1 plasmid containing human cDNA Coxsackie and Adenovirus Receptor (pCAR-DsRed). Xtreme HP Plasmid DNA transfection reagent (Roche) gave the highest transfection efficiencies compared to other tested reagents. The optimal Xtreme HP Plasmid DNA reagent had shown its higher transfection efficiency (39.25±1.00% MFI) and stable increment of DsRed-proportion cells with incubation period (24-72 hours), allowing sufficient yield and shortened the selection stably clone of successful transfected CHO-CARDsRed. Life Science Journal 2015-05-25 Article PeerReviewed application/pdf en http://irep.iium.edu.my/43121/1/Optimization_of_transfection_of_human.pdf application/pdf en http://irep.iium.edu.my/43121/2/cover_Life_Science_Journal.pdf Baba, Sayang and Elengoe, Asita and Hussein, Abdul Rahim and Sugeng , Triwahyono and Draman, Samsul and Hamdan, Salehhuddin (2015) Optimization of transfection of human coxsackie and adenovirus receptor into mammalian cells mediated by liposomes-base gene delivery. Life Science Journal, 12 (2). pp. 152-158. ISSN 1097-8135 http://www.lifesciencesite.com 10.7537/j.issn.1097-8135
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Baba, Sayang Elengoe, Asita Hussein, Abdul Rahim Sugeng , Triwahyono Draman, Samsul Hamdan, Salehhuddin Optimization of transfection of human coxsackie and adenovirus receptor into mammalian cells mediated by liposomes-base gene delivery
description	Cationic liposome-based reagents provide reliable and simple protocol for in vitro transfection in a broad range of mammalian cell types. High transfection efficacies and low cytotoxicity of a liposome-based transfection reagent was required to retain adequate viability and high level of transgene of transfected Chinese hamster ovary (CHO) cells for subsequent assay. Three commercial liposome-based transfection reagents were utilized and compared for their aptitude to transfect in-vitro CHO cells with reporter gene expressing the red fluorescent (pDsRed-N1) and fusion pDsRed-N1 plasmid containing human cDNA Coxsackie and Adenovirus Receptor (pCAR-DsRed). Xtreme HP Plasmid DNA transfection reagent (Roche) gave the highest transfection efficiencies compared to other tested reagents. The optimal Xtreme HP Plasmid DNA reagent had shown its higher transfection efficiency (39.25±1.00% MFI) and stable increment of DsRed-proportion cells with incubation period (24-72 hours), allowing sufficient yield and shortened the selection stably clone of successful transfected CHO-CARDsRed.
format	Article
author	Baba, Sayang Elengoe, Asita Hussein, Abdul Rahim Sugeng , Triwahyono Draman, Samsul Hamdan, Salehhuddin
author_facet	Baba, Sayang Elengoe, Asita Hussein, Abdul Rahim Sugeng , Triwahyono Draman, Samsul Hamdan, Salehhuddin
author_sort	Baba, Sayang
title	Optimization of transfection of human coxsackie and adenovirus receptor into mammalian cells mediated by liposomes-base gene delivery
title_short	Optimization of transfection of human coxsackie and adenovirus receptor into mammalian cells mediated by liposomes-base gene delivery
title_full	Optimization of transfection of human coxsackie and adenovirus receptor into mammalian cells mediated by liposomes-base gene delivery
title_fullStr	Optimization of transfection of human coxsackie and adenovirus receptor into mammalian cells mediated by liposomes-base gene delivery
title_full_unstemmed	Optimization of transfection of human coxsackie and adenovirus receptor into mammalian cells mediated by liposomes-base gene delivery
title_sort	optimization of transfection of human coxsackie and adenovirus receptor into mammalian cells mediated by liposomes-base gene delivery
publisher	Life Science Journal
publishDate	2015
url	http://irep.iium.edu.my/43121/ http://irep.iium.edu.my/43121/ http://irep.iium.edu.my/43121/ http://irep.iium.edu.my/43121/1/Optimization_of_transfection_of_human.pdf http://irep.iium.edu.my/43121/2/cover_Life_Science_Journal.pdf
first_indexed	2023-09-18T21:01:26Z
last_indexed	2023-09-18T21:01:26Z
_version_	1777410649773572096

Optimization of transfection of human coxsackie and adenovirus receptor into mammalian cells mediated by liposomes-base gene delivery

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