Effect of lectin from artocarpus heterophyllus seed on cancer cell lines

The objective of this paper to present cytotoxic effect of jacalin, a purified protein and crude protein from A.heterophyllus seed against cancer cell, human breast cancer (MCF7) and non-small lung carcinoma (H1299). The extraction and purification were carried out by phosphate buffer precipitatio...

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Main Authors:	Mohd Ali, Zuraidah, Abdul Munaim, Mimi Sakinah, Wan Sulaiman, Wan Mohd. Azizi
Format:	Article
Language:	English
Published:	Elsevier 2014
Subjects:	RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/43075/ http://irep.iium.edu.my/43075/ http://irep.iium.edu.my/43075/4/lectin.pdf

id	iium-43075
recordtype	eprints
spelling	iium-430752018-05-23T07:41:43Z http://irep.iium.edu.my/43075/ Effect of lectin from artocarpus heterophyllus seed on cancer cell lines Mohd Ali, Zuraidah Abdul Munaim, Mimi Sakinah Wan Sulaiman, Wan Mohd. Azizi RM Therapeutics. Pharmacology The objective of this paper to present cytotoxic effect of jacalin, a purified protein and crude protein from A.heterophyllus seed against cancer cell, human breast cancer (MCF7) and non-small lung carcinoma (H1299). The extraction and purification were carried out by phosphate buffer precipitation and reverse miceller, respectively. MTT and trypan blue exclusion methods were performed to study the cytotoxic activity. Jacalin and crude protein was compared in cytotoxic activity against MCF7 and H1299 cancer cells and the result showed jacalin more effective than crude protein, 64.73 and 75.54 percent for MCF7 and 67.71 and 68.39 percent for H1299, respectively. Jacalin also found very active against MCF7 compared to H1299 cancer cells and expected IC50 for both cancer cells would be reached if concentration of jacalin will extended in cytotoxic activity analysis. Keywords: Artocarpus heterophyllus, jacalin, crude protein, cytotoxicity,cancer cell, MCF7, H1299 Elsevier 2014-12-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/43075/4/lectin.pdf Mohd Ali, Zuraidah and Abdul Munaim, Mimi Sakinah and Wan Sulaiman, Wan Mohd. Azizi (2014) Effect of lectin from artocarpus heterophyllus seed on cancer cell lines. Journal of Life Sciences and Technologies, 2 (2). pp. 55-60. ISSN 2301-3672 http://www.jolst.net/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology
spellingShingle	RM Therapeutics. Pharmacology Mohd Ali, Zuraidah Abdul Munaim, Mimi Sakinah Wan Sulaiman, Wan Mohd. Azizi Effect of lectin from artocarpus heterophyllus seed on cancer cell lines
description	The objective of this paper to present cytotoxic effect of jacalin, a purified protein and crude protein from A.heterophyllus seed against cancer cell, human breast cancer (MCF7) and non-small lung carcinoma (H1299). The extraction and purification were carried out by phosphate buffer precipitation and reverse miceller, respectively. MTT and trypan blue exclusion methods were performed to study the cytotoxic activity. Jacalin and crude protein was compared in cytotoxic activity against MCF7 and H1299 cancer cells and the result showed jacalin more effective than crude protein, 64.73 and 75.54 percent for MCF7 and 67.71 and 68.39 percent for H1299, respectively. Jacalin also found very active against MCF7 compared to H1299 cancer cells and expected IC50 for both cancer cells would be reached if concentration of jacalin will extended in cytotoxic activity analysis. Keywords: Artocarpus heterophyllus, jacalin, crude protein, cytotoxicity,cancer cell, MCF7, H1299
format	Article
author	Mohd Ali, Zuraidah Abdul Munaim, Mimi Sakinah Wan Sulaiman, Wan Mohd. Azizi
author_facet	Mohd Ali, Zuraidah Abdul Munaim, Mimi Sakinah Wan Sulaiman, Wan Mohd. Azizi
author_sort	Mohd Ali, Zuraidah
title	Effect of lectin from artocarpus heterophyllus seed on cancer cell lines
title_short	Effect of lectin from artocarpus heterophyllus seed on cancer cell lines
title_full	Effect of lectin from artocarpus heterophyllus seed on cancer cell lines
title_fullStr	Effect of lectin from artocarpus heterophyllus seed on cancer cell lines
title_full_unstemmed	Effect of lectin from artocarpus heterophyllus seed on cancer cell lines
title_sort	effect of lectin from artocarpus heterophyllus seed on cancer cell lines
publisher	Elsevier
publishDate	2014
url	http://irep.iium.edu.my/43075/ http://irep.iium.edu.my/43075/ http://irep.iium.edu.my/43075/4/lectin.pdf
first_indexed	2023-09-18T21:01:21Z
last_indexed	2023-09-18T21:01:21Z
_version_	1777410644894547968

Effect of lectin from artocarpus heterophyllus seed on cancer cell lines

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